Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,431	G→A	F152F (TTC→TTT)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,431	0	G	A	100.0%	177.9 / NA	57	F152F (TTC→TTT)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/33);  total (24/33)

CCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAG  >  CP006881/3722295‑3722562                                                                                                                                         |                                                                                                                                    ccAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGaaaa                                                                                                                                   <  3:30989/139‑1 (MQ=255)   aatccccATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACa                                                                                                                                 <  3:409994/136‑1 (MQ=255)          aTGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAag                                                                                                                          >  7:48640/1‑139 (MQ=255)                           ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa                                                                                                          <  3:419721/138‑1 (MQ=255)                           ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa                                                                                                          <  1:291618/138‑1 (MQ=255)                           ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa                                                                                                          <  3:215308/138‑1 (MQ=255)                                aaTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAg                                                                                                         >  3:41010/1‑134 (MQ=255)                                aaTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAg                                                                                                         <  4:41010/134‑1 (MQ=255)                                 aTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCt                                                                                                   >  7:149772/1‑139 (MQ=255)                                  tCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCtt                                                                                                  >  6:386648/1‑139 (MQ=255)                                    aTGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAATCAGCTGCAAAAGCGCAGAATCCTTg                                                                                                 <  2:414718/138‑1 (MQ=255)                                       attcccccGATGTCCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa                                                                                             <  8:234521/133‑1 (MQ=255)                                       attcccccGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa                                                                                             <  8:149772/133‑1 (MQ=255)                                          cccccGATGACCGCTCCAAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATc                                                                                           <  7:191782/135‑1 (MQ=255)                                           ccccGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATcc                                                                                          >  5:385590/3‑138 (MQ=255)                                               gATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGa                                                                                     <  4:259019/139‑1 (MQ=255)                                                     cGCTCCCAATTATGGAGTGCAGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGACTCGGCAAGAAAATGAATGCTGCACTTGAAACCATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               <  5:24192/139‑1 (MQ=255)                                                     cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               <  7:46385/139‑1 (MQ=255)                                                     cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               <  3:374362/139‑1 (MQ=255)                                                     cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTCGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               <  7:85804/139‑1 (MQ=255)                                                      gCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAt                                                                              <  6:385590/139‑1 (MQ=255)                                                       cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGCATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAtt                                                                             >  1:415294/1‑139 (MQ=255)                                                       cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTCCTCATCCAATGAAACGCAtt                                                                             >  5:269928/1‑139 (MQ=255)                                                              gTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa                                                                      <  7:300294/139‑1 (MQ=255)                                                               tatggattGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATAc                                                                     <  1:115191/133‑1 (MQ=255)                                                                    attGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCag                                                                 >  5:154838/2‑137 (MQ=255)                                                                     ttGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagca                                                               <  2:105392/139‑4 (MQ=255)                                                                      tgctgcCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagcaa                                                              >  2:262364/1‑135 (MQ=255)                                                                        ctgccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa                                                                                             >  2:64509/4‑106 (MQ=255)                                                                        ctgccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa                                                                                             <  1:64509/103‑1 (MQ=255)                                                                        ctgccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagca                                                               <  2:43986/133‑4 (MQ=255)                                                                        ctgccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagca                                                               >  1:43986/4‑133 (MQ=255)                                                                         tgccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATcc                                                           >  3:241232/3‑139 (MQ=255)                                                                          gccAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATccc                                                          <  1:28877/138‑1 (MQ=255)                                                                                   ttttGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATa                                                   <  1:262364/137‑1 (MQ=255)                                                                                       gatccgcTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCg                                              <  1:226086/132‑1 (MQ=255)                                                                                       gAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCg                                              <  7:422295/138‑1 (MQ=255)                                                                                           cgcggACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGc                                         <  7:315312/135‑1 (MQ=255)                                                                                           cgcTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTgg                                          >  8:350523/3‑138 (MQ=255)                                                                                           cgcTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGc                                         <  4:241232/137‑1 (MQ=255)                                                                                            gcTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGaa                                                                                    <  2:360252/94‑1 (MQ=255)                                                                                            gcTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGaa                                                                                    >  1:360252/2‑95 (MQ=255)                                                                                                     gATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGct                               >  2:331979/1‑138 (MQ=255)                                                                                                      aTTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGctg                              >  8:9084/1‑137 (MQ=255)                                                                                                         agaaTCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTggg                            <  8:340136/136‑1 (MQ=255)                                                                                                                gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAt                                                                              <  7:62707/81‑1 (MQ=255)                                                                                                                gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAt                                                                              >  8:62707/1‑81 (MQ=255)                                                                                                                gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATCCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                     >  7:173094/1‑138 (MQ=255)                                                                                                                gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                     >  6:187088/1‑138 (MQ=255)                                                                                                                gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                     >  5:291761/1‑138 (MQ=255)                                                                                                                     aaaaTGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACaa               <  6:291761/139‑1 (MQ=255)                                                                                                                     aaaaTGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACaa               <  4:361392/139‑1 (MQ=255)                                                                                                                               ctgCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAg      >  5:147855/3‑138 (MQ=255)                                                                                                                                tgCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               >  4:377524/2‑64 (MQ=255)                                                                                                                                tgCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa                                                                               <  3:377524/63‑1 (MQ=255)                                                                                                                                  cACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGc                                         >  7:14974/1‑100 (MQ=255)                                                                                                                                  cACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGc                                         <  8:14974/100‑1 (MQ=255)                                                                                                                                  cACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAg  >  7:425294/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    CCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAG  >  CP006881/3722295‑3722562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑