Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,524	C→T	V121V (GTG→GTA)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,524	0	C	T	100.0%	280.8 / NA	91	V121V (GTG→GTA)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (59/32);  total (59/32)

GATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAA  >  CP006881/3722395‑3722656                                                                                                                                  |                                                                                                                                     gATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGct                                                                                                                             >  2:331979/1‑138 (MQ=255)  aTTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGctg                                                                                                                            >  8:9084/1‑137 (MQ=255)     agaaTCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTggg                                                                                                                          <  8:340136/136‑1 (MQ=255)            gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATCCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                                                                                                                   >  7:173094/1‑138 (MQ=255)            gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                                                                                                                   >  5:291761/1‑138 (MQ=255)            gCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTg                                                                                                                   >  6:187088/1‑138 (MQ=255)                 aaaaTGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACaa                                                                                                             <  4:361392/139‑1 (MQ=255)                 aaaaTGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACaa                                                                                                             <  6:291761/139‑1 (MQ=255)                           ctgCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAg                                                                                                    >  5:147855/3‑138 (MQ=255)                              cACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAg                                                                                                >  7:425294/1‑139 (MQ=255)                                   gaaaaCATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGttt                                                                                           >  4:296207/4‑139 (MQ=255)                                      aaCATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCg                                                                                        >  8:247163/1‑139 (MQ=255)                                           atcaagcagcTGCAAAGGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCAATTAATAGCCCGCTGGCTACAAGAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAAt                                                                                   >  2:247301/5‑139 (MQ=255)                                           atcaagcagcTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAAt                                                                                   >  6:428848/5‑139 (MQ=255)                                                  agcTGCAAAAGCGCAGAATCCTTGCTCATCGAATGAAAAGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAg                                                                            <  2:209822/139‑1 (MQ=255)                                                    cTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAACTCTCGCAGCg                                                                          >  3:402719/1‑139 (MQ=255)                                                    cTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCg                                                                          >  1:62959/1‑139 (MQ=255)                                                    cTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCg                                                                          >  1:21064/1‑139 (MQ=255)                                                         aaagcGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGAtt                                                                     <  3:296207/137‑1 (MQ=255)                                                             cgcAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAgc                                                                 <  8:425294/139‑2 (MQ=255)                                                               cAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTTCAATAGCTTGGATGATTGGAACATAAATATTAGAAAGCGTTATCGCAAATCTCGCAGCGGGATTCAGctt                                                               <  2:415294/139‑1 (MQ=255)                                                               cAGAATCCGTGCTCATCCAATGAACCGCCTTGAGCCTACCCAGCTCTCCCATTAATAGGCCGCGGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGctt                                                               <  6:269928/139‑1 (MQ=255)                                                                cagaTCCTTGCTCATCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACGATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGctt                                                               <  4:448193/135‑1 (MQ=255)                                                                             aTCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTtgccgc                                                   <  5:187088/137‑1 (MQ=255)                                                                              tgcAATGAAACGCATTGATCATACCCAGGAATCCCATTAATAGGCCGCTGTCTACAATAGGTGGGATGATTTGATCAAAAATATTAGAAAGAGTTTTAGCAAATCTCGCAGCGTTATTCAGCTTTTGTTTTGCCGCATc                                                <  4:326644/137‑1 (MQ=255)                                                                              tCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATc                                                >  8:216738/1‑139 (MQ=255)                                                                              tCCAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATc                                                >  5:153912/1‑139 (MQ=255)                                                                               ccAATGAAACGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATcc                                               >  8:78677/1‑139 (MQ=255)                                                                                cAATGAAACGCATTGATCATACCCAGCACTCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCt                                              <  8:48640/139‑1 (MQ=255)                                                                                     aaaCGCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCtgatga                                         <  1:331979/139‑1 (MQ=255)                                                                                        cGCATTGATCATACCCATCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGAttg                                      <  4:295781/139‑2 (MQ=255)                                                                                         gCATTGATCATACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGAttga                                     >  5:364051/1‑137 (MQ=255)                                                                                                   tACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAg                                                                                                                               >  4:46048/1‑39 (MQ=255)                                                                                                   tACCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAg                                                                                                                               <  3:46048/39‑1 (MQ=255)                                                                                                    aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCt                                              <  2:419465/119‑1 (MQ=255)                                                                                                    aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCt                                              >  1:419465/1‑119 (MQ=255)                                                                                                    aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          >  8:127158/1‑139 (MQ=255)                                                                                                    aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          >  5:161498/1‑139 (MQ=255)                                                                                                     cccagcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTCTGCCGCATCCTGATGATTGACGTGTTCTTCa                          <  8:173094/132‑1 (MQ=255)                                                                                                      ccagcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACg                        <  7:9084/134‑1 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCt                                              <  4:389449/112‑1 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCt                                              >  3:389449/3‑114 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACTTGTTCTTCACGCTc                     >  7:404237/3‑139 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      <  8:339891/136‑1 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      >  7:339891/3‑138 (MQ=255)                                                                                                         gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTc                     >  2:13335/3‑139 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGTAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGCCGCATCCTGATGATTGCCGAGTTCTTCACGCTc                     >  8:212980/2‑138 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAACTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                    >  6:443479/2‑138 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCCGCTTTTCTGTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                    >  6:196138/2‑138 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTtgc                                                      >  3:399295/2‑105 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTtgc                                                      <  4:399295/104‑1 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          <  4:235229/132‑1 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          >  3:235229/2‑133 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                    <  7:212980/138‑2 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTc                     >  8:126073/2‑138 (MQ=255)                                                                                                          caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGACGCATCCTGATGATTGACGTGTTCTTCACGCTc                     >  3:121375/2‑138 (MQ=255)                                                                                                             tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                 >  7:76236/1‑135 (MQ=255)                                                                                                             tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                 >  4:98857/1‑135 (MQ=255)                                                                                                             tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                 >  2:58393/1‑135 (MQ=255)                                                                                                                  ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGc            >  6:315684/1‑139 (MQ=255)                                                                                                                  ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGc            <  6:154838/139‑1 (MQ=255)                                                                                                                  ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGAAGATTGACGTGTTCTTCACGCTCGATATCatc            >  4:338315/1‑137 (MQ=255)                                                                                                                       aGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt       >  6:411781/1‑137 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTtgcc                                                     <  2:241040/91‑2 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTtgcc                                                     >  1:241040/1‑90 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACgtg                                 >  8:408067/1‑111 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACgtg                                 <  7:408067/111‑1 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      >  6:202449/1‑122 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      <  5:202449/122‑1 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt       >  7:7201/1‑135 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt       >  8:43340/1‑135 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt       >  8:76607/1‑135 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCttt      <  7:127376/138‑1 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCttt      >  8:127376/1‑138 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGAATCCTGATGATTGACGTGTTCTTCTCGCTCGATATCAGCTTctt       >  5:138138/1‑135 (MQ=255)                                                                                                                         gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTACGCTTTTCTTTTGCCGAATCCTGATGATTGACGTGTTCTTCACTCTCGATATCAGCTTCTTTa     >  5:337539/1‑139 (MQ=255)                                                                                                                         gCCGCTGGCTACAAAAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGATATTCTTTTGCCGCATCCAGATGATTGACGTGTTCTTCACGCTCGAAATCAGCttattt      >  8:248226/1‑134 (MQ=255)                                                                                                                          ccGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt       >  4:414834/1‑134 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCtgatga                                         <  8:121609/101‑1 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCtgatga                                         >  7:121609/1‑101 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGAttg                                      >  2:358849/1‑103 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGAttg                                      <  1:358849/104‑2 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      <  7:216087/120‑1 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                      >  8:216087/1‑120 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg    >  2:429851/1‑138 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg    >  4:152912/1‑138 (MQ=255)                                                                                                                           cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg    >  6:299682/1‑138 (MQ=255)                                                                                                                             cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          >  5:6740/1‑114 (MQ=255)                                                                                                                             cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                          <  6:6740/114‑1 (MQ=255)                                                                                                                             cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGaa  >  3:62107/1‑138 (MQ=255)                                                                                                                             cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGCCGCATCCTGATGATTGACGTGTTCTTAACGCTCGATATAAGCTTCGTTATaa  >  6:52606/1‑138 (MQ=255)                                                                                                                                  |                                                                                                                                     GATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAA  >  CP006881/3722395‑3722656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑