Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,620	T→G	V89V (GTA→GTC)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,620	0	T	G	100.0%	309.4 / NA	96	V89V (GTA→GTC)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (48/48);  total (48/48)

ACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTT  >  CP006881/3722494‑3722750                                                                                                                               |                                                                                                                                   aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        >  5:161498/1‑139 (MQ=255) aCCCAGCAATCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        >  8:127158/1‑139 (MQ=255)  cccagcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTCTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        <  8:173094/132‑1 (MQ=255)   ccagcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACg                                                                                                                      <  7:9084/134‑1 (MQ=255)      gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACTTGTTCTTCACGCTc                                                                                                                   >  7:404237/3‑139 (MQ=255)      gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    <  8:339891/136‑1 (MQ=255)      gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    >  7:339891/3‑138 (MQ=255)      gcaatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTc                                                                                                                   >  2:13335/3‑139 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGTAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGCCGCATCCTGATGATTGCCGAGTTCTTCACGCTc                                                                                                                   >  8:212980/2‑138 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAACTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                                                                                                                  >  6:443479/2‑138 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCCGCTTTTCTGTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                                                                                                                  >  6:196138/2‑138 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        <  4:235229/132‑1 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        >  3:235229/2‑133 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcg                                                                                                                  <  7:212980/138‑2 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTc                                                                                                                   >  8:126073/2‑138 (MQ=255)       caatCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGACGCATCCTGATGATTGACGTGTTCTTCACGCTc                                                                                                                   >  3:121375/2‑138 (MQ=255)          tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                                                                                                               >  7:76236/1‑135 (MQ=255)          tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                                                                                                               >  2:58393/1‑135 (MQ=255)          tCCCATTAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTcgata                                                                                                               >  4:98857/1‑135 (MQ=255)               ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGc                                                                                                          <  6:154838/139‑1 (MQ=255)               ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGc                                                                                                          >  6:315684/1‑139 (MQ=255)               ttAATAGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGAAGATTGACGTGTTCTTCACGCTCGATATCatc                                                                                                          >  4:338315/1‑137 (MQ=255)                    aGGCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt                                                                                                     >  6:411781/1‑137 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACgtg                                                                                                                               <  7:408067/111‑1 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACgtg                                                                                                                               >  8:408067/1‑111 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    >  6:202449/1‑122 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    <  5:202449/122‑1 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt                                                                                                     >  8:76607/1‑135 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt                                                                                                     >  7:7201/1‑135 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt                                                                                                     >  8:43340/1‑135 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCttt                                                                                                    >  8:127376/1‑138 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCttt                                                                                                    <  7:127376/138‑1 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGAATCCTGATGATTGACGTGTTCTTCTCGCTCGATATCAGCTTctt                                                                                                     >  5:138138/1‑135 (MQ=255)                      gCCGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTACGCTTTTCTTTTGCCGAATCCTGATGATTGACGTGTTCTTCACTCTCGATATCAGCTTCTTTa                                                                                                   >  5:337539/1‑139 (MQ=255)                      gCCGCTGGCTACAAAAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGATATTCTTTTGCCGCATCCAGATGATTGACGTGTTCTTCACGCTCGAAATCAGCttattt                                                                                                    >  8:248226/1‑134 (MQ=255)                       ccGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTctt                                                                                                     >  4:414834/1‑134 (MQ=255)                        cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    <  7:216087/120‑1 (MQ=255)                        cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCt                                                                                                                    >  8:216087/1‑120 (MQ=255)                        cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg                                                                                                  >  2:429851/1‑138 (MQ=255)                        cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg                                                                                                  >  6:299682/1‑138 (MQ=255)                        cGCTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAg                                                                                                  >  4:152912/1‑138 (MQ=255)                          cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        <  6:6740/114‑1 (MQ=255)                          cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCa                                                                                                                        >  5:6740/1‑114 (MQ=255)                          cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGaa                                                                                                >  3:62107/1‑138 (MQ=255)                          cTGGCTACAATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTATTGCCGCATCCTGATGATTGACGTGTTCTTAACGCTCGATATAAGCTTCGTTATaa                                                                                                >  6:52606/1‑138 (MQ=255)                                 caATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATc                                                                                        >  1:195632/1‑139 (MQ=255)                                  aaTAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATc                                                                                        >  3:35280/1‑138 (MQ=255)                                  aaTAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCg                                                                                       <  7:350523/139‑1 (MQ=255)                                    tAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                     <  5:411781/139‑3 (MQ=255)                                      gctgggATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaat                                                                                    <  8:76236/135‑4 (MQ=255)                                          ggATGATTGGAACAAAAATCTTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaatact                                                                                 <  7:248226/137‑7 (MQ=255)                                           gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                              <  1:429851/139‑1 (MQ=255)                                           gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                              >  6:86108/1‑139 (MQ=255)                                           gatgatTGGAACAAAAATATTAGAAAGCGTTATCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                              <  1:247301/139‑1 (MQ=255)                                            atgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAt                                                                             >  5:355378/1‑139 (MQ=255)                                               atTGGAACAAAAATATAAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAttca                                                                          >  4:93485/1‑137 (MQ=255)                                                ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                         <  5:299682/139‑1 (MQ=255)                                                ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGATTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                         <  2:21064/139‑1 (MQ=255)                                                              ttAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTACTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCg                                                            >  8:310166/1‑138 (MQ=255)                                                                aGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaa                                                          <  6:364051/138‑1 (MQ=255)                                                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                        <  3:152912/139‑1 (MQ=255)                                                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                        <  4:86254/139‑1 (MQ=255)                                                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                        <  7:216738/139‑1 (MQ=255)                                                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   <  7:43340/139‑1 (MQ=255)                                                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   >  2:443261/1‑139 (MQ=255)                                                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   >  7:129545/1‑139 (MQ=255)                                                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   <  3:414834/139‑1 (MQ=255)                                                                         tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   >  7:152874/1‑136 (MQ=255)                                                                         tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   <  8:152874/136‑1 (MQ=255)                                                                          ttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTgg                                                >  8:21541/1‑138 (MQ=255)                                                                            cGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTa                                              <  5:315684/138‑1 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                     <  5:80681/95‑3 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                     >  6:80681/1‑93 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                         >  5:225846/1‑139 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                         <  5:428848/139‑1 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                         <  6:325235/139‑1 (MQ=255)                                                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                         <  7:126073/139‑1 (MQ=255)                                                                                     cGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                    <  1:58393/139‑1 (MQ=255)                                                                                      gCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                    <  2:195632/138‑1 (MQ=255)                                                                                             gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   <  4:315973/116‑1 (MQ=255)                                                                                             gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                   >  3:315973/1‑116 (MQ=255)                                                                                              aTTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGaaa                           <  7:76607/139‑1 (MQ=255)                                                                                               ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                          <  8:129545/139‑1 (MQ=255)                                                                                               ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                          <  1:443261/139‑1 (MQ=255)                                                                                                 cAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                         <  6:355378/138‑1 (MQ=255)                                                                                                       ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                         >  6:358845/1‑132 (MQ=255)                                                                                                       ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                         <  5:358845/132‑1 (MQ=255)                                                                                                         cTTTTGCCGCATCCTGATGATTGTCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                 <  3:395834/138‑3 (MQ=255)                                                                                                         cTTTTGCCGCATCCTGATGATTGACTTGTTCTTCACGCTCGATATCAGGTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                 <  6:138138/138‑3 (MQ=255)                                                                                                                cgcATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa         <  4:62107/138‑1 (MQ=255)                                                                                                                  cATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAg       <  6:225846/139‑1 (MQ=255)                                                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGTACAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc      <  6:147855/139‑1 (MQ=255)                                                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc      <  3:93485/139‑1 (MQ=255)                                                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc      <  7:247163/139‑1 (MQ=255)                                                                                                                      cTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGctct   <  3:98857/139‑1 (MQ=255)                                                                                                                         atGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCtctt  <  2:62959/136‑1 (MQ=255)                                                                                                                               |                                                                                                                                   ACCCAATAATCCCATTAATAGGCCGCTGGCCACAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTT  >  CP006881/3722494‑3722750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑