Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,659	A→G	A76A (GCT→GCC)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,659	0	A	G	100.0%	225.4 / NA	70	A76A (GCT→GCC)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/49);  total (21/49)

CAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTA  >  CP006881/3722526‑3722792                                                                                                                                      |                                                                                                                                      caATAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATc                                                                                                                                  >  1:195632/1‑139 (MQ=255)  aaTAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATc                                                                                                                                  >  3:35280/1‑138 (MQ=255)  aaTAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCg                                                                                                                                 <  7:350523/139‑1 (MQ=255)    tAGCTGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                                                               <  5:411781/139‑3 (MQ=255)      gctgggATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaat                                                                                                                              <  8:76236/135‑4 (MQ=255)          ggATGATTGGAACAAAAATCTTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaatact                                                                                                                           <  7:248226/137‑7 (MQ=255)           gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                        <  1:429851/139‑1 (MQ=255)           gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                        >  6:86108/1‑139 (MQ=255)           gatgatTGGAACAAAAATATTAGAAAGCGTTATCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                        <  1:247301/139‑1 (MQ=255)            atgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAt                                                                                                                       >  5:355378/1‑139 (MQ=255)               atTGGAACAAAAATATAAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAttca                                                                                                                    >  4:93485/1‑137 (MQ=255)                ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                                                                   <  5:299682/139‑1 (MQ=255)                ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGATTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                                                                   <  2:21064/139‑1 (MQ=255)                              ttAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTACTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCg                                                                                                      >  8:310166/1‑138 (MQ=255)                                aGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaa                                                                                                    <  6:364051/138‑1 (MQ=255)                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                  <  3:152912/139‑1 (MQ=255)                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                  <  4:86254/139‑1 (MQ=255)                                 gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                  <  7:216738/139‑1 (MQ=255)                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             <  3:414834/139‑1 (MQ=255)                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             >  7:129545/1‑139 (MQ=255)                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             >  2:443261/1‑139 (MQ=255)                                      cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             <  7:43340/139‑1 (MQ=255)                                         tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             >  7:152874/1‑136 (MQ=255)                                         tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             <  8:152874/136‑1 (MQ=255)                                          ttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTgg                                                                                          >  8:21541/1‑138 (MQ=255)                                            cGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTa                                                                                        <  5:315684/138‑1 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                                                               >  6:80681/1‑93 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCgaa                                                                                                                               <  5:80681/95‑3 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                   >  5:225846/1‑139 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                   <  7:126073/139‑1 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                   <  5:428848/139‑1 (MQ=255)                                                aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                   <  6:325235/139‑1 (MQ=255)                                                     cGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                              <  1:58393/139‑1 (MQ=255)                                                      gCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                              <  2:195632/138‑1 (MQ=255)                                                             gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             >  3:315973/1‑116 (MQ=255)                                                             gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                             <  4:315973/116‑1 (MQ=255)                                                              aTTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGaaa                                                                     <  7:76607/139‑1 (MQ=255)                                                               ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                                                                    <  1:443261/139‑1 (MQ=255)                                                               ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                                                                    <  8:129545/139‑1 (MQ=255)                                                                 cAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                   <  6:355378/138‑1 (MQ=255)                                                                       ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                   <  5:358845/132‑1 (MQ=255)                                                                       ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                   >  6:358845/1‑132 (MQ=255)                                                                         cTTTTGCCGCATCCTGATGATTGTCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                                                           <  3:395834/138‑3 (MQ=255)                                                                         cTTTTGCCGCATCCTGATGATTGACTTGTTCTTCACGCTCGATATCAGGTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                                                           <  6:138138/138‑3 (MQ=255)                                                                                cgcATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                   <  4:62107/138‑1 (MQ=255)                                                                                  cATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAg                                                 <  6:225846/139‑1 (MQ=255)                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGTACAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                <  6:147855/139‑1 (MQ=255)                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                <  7:247163/139‑1 (MQ=255)                                                                                   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                <  3:93485/139‑1 (MQ=255)                                                                                      cTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGctct                                             <  3:98857/139‑1 (MQ=255)                                                                                         atGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCtctt                                            <  2:62959/136‑1 (MQ=255)                                                                                              tgaCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                              <  3:35439/96‑1 (MQ=255)                                                                                              tgaCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                              >  4:35439/3‑98 (MQ=255)                                                                                                aCGGGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCGGAAAGCCCCTTTACCGCCATCAAGCTCTTCTACTtgtg                                   >  3:139431/1‑139 (MQ=255)                                                                                                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGTATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                             <  3:338315/138‑1 (MQ=255)                                                                                                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                             >  4:393704/1‑138 (MQ=255)                                                                                                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                             <  3:393704/138‑1 (MQ=255)                                                                                                          cACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCACTTTAACGCCATCAAGCTCTTCTACTTGTGGTTGatc                            <  5:52606/136‑1 (MQ=255)                                                                                                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                   >  3:80342/2‑89 (MQ=255)                                                                                                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                   <  4:80342/88‑1 (MQ=255)                                                                                                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                             >  6:139698/2‑127 (MQ=255)                                                                                                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                             <  5:139698/126‑1 (MQ=255)                                                                                                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGAtt                 <  4:35280/138‑1 (MQ=255)                                                                                                                     tCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCa               <  4:121375/138‑1 (MQ=255)                                                                                                                           tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                         >  6:162298/4‑90 (MQ=255)                                                                                                                           tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                         <  5:162298/87‑1 (MQ=255)                                                                                                                            ctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtca        <  5:86108/136‑1 (MQ=255)                                                                                                                             tttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcat       >  6:150514/2‑138 (MQ=255)                                                                                                                               tAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcatc      <  7:310166/137‑1 (MQ=255)                                                                                                                                 gAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACt   <  7:21541/138‑1 (MQ=255)                                                                                                                                  aaaagGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTa  >  8:263564/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                      CAATAGCCGGGATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTA  >  CP006881/3722526‑3722792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑