Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,668	C→T	V73V (GTG→GTA)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,668	0	C	T	100.0%	210.3 / NA	68	V73V (GTG→GTA)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/48);  total (20/48)

GATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAG  >  CP006881/3722536‑3722799                                                                                                                                     |                                                                                                                                    gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                               <  1:429851/139‑1 (MQ=255) gatgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                               >  6:86108/1‑139 (MQ=255) gatgatTGGAACAAAAATATTAGAAAGCGTTATCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTa                                                                                                                               <  1:247301/139‑1 (MQ=255)  atgatTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAt                                                                                                                              >  5:355378/1‑139 (MQ=255)     atTGGAACAAAAATATAAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTAttca                                                                                                                           >  4:93485/1‑137 (MQ=255)      ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                                                                          <  5:299682/139‑1 (MQ=255)      ttGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGATTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCAc                                                                                                                          <  2:21064/139‑1 (MQ=255)                    ttAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTACTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCg                                                                                                             >  8:310166/1‑138 (MQ=255)                      aGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaa                                                                                                           <  6:364051/138‑1 (MQ=255)                       gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                         <  4:86254/139‑1 (MQ=255)                       gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                         <  3:152912/139‑1 (MQ=255)                       gAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGaaaa                                                                                                         <  7:216738/139‑1 (MQ=255)                            cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    >  7:129545/1‑139 (MQ=255)                            cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    >  2:443261/1‑139 (MQ=255)                            cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    <  7:43340/139‑1 (MQ=255)                            cGTTTTCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    <  3:414834/139‑1 (MQ=255)                               tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    >  7:152874/1‑136 (MQ=255)                               tttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    <  8:152874/136‑1 (MQ=255)                                ttCGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTgg                                                                                                 >  8:21541/1‑138 (MQ=255)                                  cGCAAATCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTa                                                                                               <  5:315684/138‑1 (MQ=255)                                      aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                          <  5:428848/139‑1 (MQ=255)                                      aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                          <  7:126073/139‑1 (MQ=255)                                      aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                          <  6:325235/139‑1 (MQ=255)                                      aaTCTCGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGc                                                                                          >  5:225846/1‑139 (MQ=255)                                           cGCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                                     <  1:58393/139‑1 (MQ=255)                                            gCAGCGGGATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                                     <  2:195632/138‑1 (MQ=255)                                                   gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    <  4:315973/116‑1 (MQ=255)                                                   gATTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATc                                                                                                    >  3:315973/1‑116 (MQ=255)                                                    aTTCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGaaa                                                                            <  7:76607/139‑1 (MQ=255)                                                     ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                                                                           <  8:129545/139‑1 (MQ=255)                                                     ttCAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAg                                                                           <  1:443261/139‑1 (MQ=255)                                                       cAGCTTTTCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                          <  6:355378/138‑1 (MQ=255)                                                             ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                          >  6:358845/1‑132 (MQ=255)                                                             ttCTTTTGCCGCATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                          <  5:358845/132‑1 (MQ=255)                                                               cTTTTGCCGCATCCTGATGATTGTCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                                                                  <  3:395834/138‑3 (MQ=255)                                                               cTTTTGCCGCATCCTGATGATTGACTTGTTCTTCACGCTCGATATCAGGTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCtttaa                                                                  <  6:138138/138‑3 (MQ=255)                                                                      cgcATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                          <  4:62107/138‑1 (MQ=255)                                                                        cATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAg                                                        <  6:225846/139‑1 (MQ=255)                                                                         aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGTACAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                       <  6:147855/139‑1 (MQ=255)                                                                         aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                       <  7:247163/139‑1 (MQ=255)                                                                         aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                       <  3:93485/139‑1 (MQ=255)                                                                            cTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGctct                                                    <  3:98857/139‑1 (MQ=255)                                                                               atGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCtctt                                                   <  2:62959/136‑1 (MQ=255)                                                                                    tgaCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                                     >  4:35439/3‑98 (MQ=255)                                                                                    tgaCGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCagag                                                                                     <  3:35439/96‑1 (MQ=255)                                                                                      aCGGGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCGGAAAGCCCCTTTACCGCCATCAAGCTCTTCTACTtgtg                                          >  3:139431/1‑139 (MQ=255)                                                                                             cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGTATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                    <  3:338315/138‑1 (MQ=255)                                                                                             cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                    >  4:393704/1‑138 (MQ=255)                                                                                             cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                    <  3:393704/138‑1 (MQ=255)                                                                                                cACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCACTTTAACGCCATCAAGCTCTTCTACTTGTGGTTGatc                                   <  5:52606/136‑1 (MQ=255)                                                                                                        atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                          >  3:80342/2‑89 (MQ=255)                                                                                                        atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAgc                                                                          <  4:80342/88‑1 (MQ=255)                                                                                                        atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                    <  5:139698/126‑1 (MQ=255)                                                                                                        atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                    >  6:139698/2‑127 (MQ=255)                                                                                                        atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGAtt                        <  4:35280/138‑1 (MQ=255)                                                                                                           tCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCa                      <  4:121375/138‑1 (MQ=255)                                                                                                                 tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                                <  5:162298/87‑1 (MQ=255)                                                                                                                 tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                                >  6:162298/4‑90 (MQ=255)                                                                                                                  ctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtca               <  5:86108/136‑1 (MQ=255)                                                                                                                   tttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcat              >  6:150514/2‑138 (MQ=255)                                                                                                                     tAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcatc             <  7:310166/137‑1 (MQ=255)                                                                                                                       gAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACt          <  7:21541/138‑1 (MQ=255)                                                                                                                        aaaagGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTa         >  8:263564/1‑138 (MQ=255)                                                                                                                           aggCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg     <  8:404237/137‑1 (MQ=255)                                                                                                                             gCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg     >  2:386146/1‑137 (MQ=255)                                                                                                                              cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg  <  7:78677/139‑1 (MQ=255)                                                                                                                              cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg  <  6:161498/139‑1 (MQ=255)                                                                                                                              cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg  <  1:13335/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    GATGATTGGAACAAAAATATTAGAAAGCGTTTTCGCAAATCTCGCAGCGGGATTCAGTTTTTCTTTTGCTGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAG  >  CP006881/3722536‑3722799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑