Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,722,734 T→A V51V (GTA→GTT)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,722,7340TA97.8% 289.2 / ‑4.4 93V51V (GTA→GTT) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (46/44);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATGTTTTCTTTTCCCCCGAGAAGTTCAATGAGGCGTTTTGCA  >  CP006881/3722605‑3722869
                                                                                                                                 |                                                                                                                                       
cgcATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                                                                                                <  4:62107/138‑1 (MQ=255)
  cATCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAg                                                                                                                              <  6:225846/139‑1 (MQ=255)
   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGTACAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                                                                                             <  6:147855/139‑1 (MQ=255)
   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                                                                                             <  7:247163/139‑1 (MQ=255)
   aTCCTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGc                                                                                                                             <  3:93485/139‑1 (MQ=255)
      cTGATGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGctct                                                                                                                          <  3:98857/139‑1 (MQ=255)
         atGATTGACGTGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCtctt                                                                                                                         <  2:62959/136‑1 (MQ=255)
                aCGGGTTCTTCACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCGGAAAGCCCCTTTACCGCCATCAAGCTCTTCTACTtgtg                                                                                                                >  3:139431/1‑139 (MQ=255)
                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGTATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          <  3:338315/138‑1 (MQ=255)
                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          <  3:393704/138‑1 (MQ=255)
                       cttcACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          >  4:393704/1‑138 (MQ=255)
                          cACGCTCGATATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCACTTTAACGCCATCAAGCTCTTCTACTTGTGGTTGatc                                                                                                         <  5:52606/136‑1 (MQ=255)
                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          <  5:139698/126‑1 (MQ=255)
                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          >  6:139698/2‑127 (MQ=255)
                                  atATCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGAtt                                                                                              <  4:35280/138‑1 (MQ=255)
                                     tCAGCTTCTTTAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCa                                                                                            <  4:121375/138‑1 (MQ=255)
                                           tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                                                                                                      >  6:162298/4‑90 (MQ=255)
                                           tctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACg                                                                                                                                      <  5:162298/87‑1 (MQ=255)
                                            ctttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtca                                                                                     <  5:86108/136‑1 (MQ=255)
                                             tttAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcat                                                                                    >  6:150514/2‑138 (MQ=255)
                                               tAGAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTtcatc                                                                                   <  7:310166/137‑1 (MQ=255)
                                                 gAAAAGGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACt                                                                                <  7:21541/138‑1 (MQ=255)
                                                  aaaagGCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTa                                                                               >  8:263564/1‑138 (MQ=255)
                                                     aggCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg                                                                           <  8:404237/137‑1 (MQ=255)
                                                       gCATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg                                                                           >  2:386146/1‑137 (MQ=255)
                                                        cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg                                                                        <  6:161498/139‑1 (MQ=255)
                                                        cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg                                                                        <  1:13335/139‑1 (MQ=255)
                                                        cATCGAATACTTTATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg                                                                        <  7:78677/139‑1 (MQ=255)
                                                               tactttATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCttgtt                                                                 <  7:51764/135‑1 (MQ=255)
                                                                  tttATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGca                                                              >  5:216603/2‑139 (MQ=255)
                                                                    tATTCACAAGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGcac                                                             >  2:122824/1‑138 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                                                                                                <  3:19348/64‑1 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                                                                                                >  4:19348/1‑64 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGcc                                                     >  3:194063/1‑139 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGcc                                                     >  8:374717/1‑139 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGcc                                                     >  1:409353/1‑139 (MQ=255)
                                                                           aaGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGcc                                                     >  8:243702/1‑139 (MQ=255)
                                                                            aGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCttgtt                                                                 <  4:282646/126‑1 (MQ=255)
                                                                            aGGCCTGTTCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCttgtt                                                                 >  3:282646/1‑126 (MQ=255)
                                                                             ggCCGGTTGCGAAAATGATCTGGGACTGGCCAGAGCTGCTGAAAGCCCCTTTCACGCCCTCAAGCGCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAACCGCAGTCTTGTTGCACAATGAGCcgc                                                   <  8:335134/139‑1 (MQ=255)
                                                                                   ttCCGAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGa                                              >  3:193526/1‑138 (MQ=255)
                                                                                       gAAAATGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAAta                                         <  7:263564/139‑2 (MQ=255)
                                                                                        aaaaTGATCTGGTACTGGCCAGATCTTCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATATTTGATTCATCTTTCATAACTAAACGAAGACTAGTTGCACAATGAGCAGCGCACATAAtat                                        >  5:231691/1‑137 (MQ=255)
                                                                                        aaaaTGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                                                                                                >  8:280318/1‑51 (MQ=255)
                                                                                        aaaaTGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCa                                                                                                                                <  7:280318/51‑1 (MQ=255)
                                                                                        aaaaTGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTaaa                                                                             >  7:437943/1‑102 (MQ=255)
                                                                                        aaaaTGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTaaa                                                                             <  8:437943/102‑1 (MQ=255)
                                                                                         aaaTGATCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTAGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAtt                                       <  4:402719/139‑1 (MQ=255)
                                                                                             gatCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAttttct                                   >  1:282098/2‑139 (MQ=255)
                                                                                             gatCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGGGCTTGATCTATCTTTGATTCATCTTTCATCACTACACGCAGTCTTGTTGCAGAATGAGCGGCGGTTATAATAttttgt                                   >  5:281257/2‑137 (MQ=255)
                                                                                             gatCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGACATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAttttct                                   >  2:289299/2‑139 (MQ=255)
                                                                                               tCTGGTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAACCGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAttttctt                                  >  2:177480/1‑138 (MQ=255)
                                                                                                   ggaCTGGCCAGAGCTGCTGAAAGCCACTTTAACGCCAGCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCAGCGCGGATAATATTTTCTTTccct                             <  4:139431/137‑2 (MQ=255)
                                                                                                   gTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAttttctttcc                               <  7:127158/137‑3 (MQ=255)
                                                                                                   gTACTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATAttttctt                                  <  2:409353/134‑1 (MQ=255)
                                                                                                      cTGGCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCcctcc                           >  2:134349/1‑138 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg                                                                           <  4:410142/87‑1 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACg                                                                           >  3:410142/1‑87 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCtt                                                                    <  8:74550/94‑1 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCtt                                                                    >  7:74550/1‑94 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCgag                        >  7:302670/1‑138 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCgag                        >  6:29979/1‑138 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCgag                        >  1:41503/1‑138 (MQ=255)
                                                                                                         gCCAGAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGAGTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGCTAATATTTTCTTTCACTCCgag                        >  4:316245/1‑138 (MQ=255)
                                                                                                         gCCAAAGCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCgag                        >  2:25662/1‑138 (MQ=255)
                                                                                                           caaagCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAg                     <  1:25662/136‑1 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGc                                                                                                               >  4:174614/1‑46 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGc                                                                                                               <  3:174614/46‑1 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAAta                                         <  2:413367/116‑2 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAAta                                         >  1:413367/1‑115 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAgct                   >  2:40049/1‑136 (MQ=255)
                                                                                                              agCTGCTGAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTAGATTCATCTTTCATCACTAAACGCAGTATTGTTGAACAATGAGCCGCGCTGATAATATATTCTTTCCCTCCGAGAAgct                   >  8:277237/1‑136 (MQ=255)
                                                                                                                   ctgAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg                                                                        <  2:222051/80‑1 (MQ=255)
                                                                                                                   ctgAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAg                                                                        >  1:222051/1‑80 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          <  3:319913/46‑1 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGat                                                                                                          >  4:319913/1‑46 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCttgttg                                                                >  5:197185/1‑88 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCttgttg                                                                <  6:197185/88‑1 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATTAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt               >  8:438659/1‑137 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCcgc                                                   >  2:207544/1‑101 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCcgc                                                   <  1:207544/101‑1 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt               >  1:17223/1‑137 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt               >  5:123704/1‑137 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt               >  5:436735/1‑137 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg              <  8:7201/138‑1 (MQ=255)
                                                                                                                   ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg              <  1:289299/138‑1 (MQ=255)
                                                                                                                      taaGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg            <  6:337539/136‑1 (MQ=255)
                                                                                                                        aGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGt        >  1:62523/1‑139 (MQ=255)
                                                                                                                        aGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGt        >  2:62029/1‑139 (MQ=255)
                                                                                                                          ccccTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTCAAAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGTCGtat      >  2:217621/3‑137 (MQ=255)
                                                                                                                            gcTTTAACGCCATGAAGCTCTTCTACTTGTGCTTGATATATCTTTGATTCATCTTTCATCACTAAACTGAGTCTTGTTGCACAATGAGGCGCGATGATAATATTTTCTTTCGCTCCGAGAAGCTCAATGAGGCGTTTTg    <  6:231691/138‑1 (MQ=255)
                                                                                                                            ccTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTTATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg    <  7:438659/139‑1 (MQ=255)
                                                                                                                             cTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCGTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg    >  3:200155/1‑138 (MQ=255)
                                                                                                                              tttaACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAACCTCAATGAGGCGTTTTGCa  >  3:12222/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                       
TGCATCCTGGTGATTTACGTGTTCTTCACGCTCAGTATCAGCTTCCTTAGAAAAAGCATCGTACACCTTATTGACAAGGCCTGTTCCGAAAATAATCTGGTACTGGCCAGAGCTGCTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATGTTTTCTTTTCCCCCGAGAAGTTCAATGAGGCGTTTTGCA  >  CP006881/3722605‑3722869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑