Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,284	C→T	T214T (ACG→ACA)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,284	0	C	T	100.0%	90.8 / NA	30	T214T (ACG→ACA)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

AATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACATGGGATAGGAGTGATTTTTCTTTCGTGATGGCTTCTTTTGTTTCTAGTGAAAGCGT  >  CP006881/2174155‑2174415                                                                                                                                  |                                                                                                                                    aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAg                                                                                                                             <  4:94325/138‑1 (MQ=255)       aaGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTgg                                                                                                                      >  7:102138/1‑139 (MQ=255)         gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                                                    <  7:11422/137‑1 (MQ=255)         gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                                                    <  6:100129/137‑1 (MQ=255)         gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                                                    >  5:38443/3‑139 (MQ=255)           gcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCgag                                                                                                                  <  7:19512/139‑3 (MQ=255)                         ctCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGTTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTATGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTg                                                                                                     >  6:275786/2‑138 (MQ=255)                               ggATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTgcagcaat                                                                                              <  4:241331/139‑3 (MQ=255)                                   gTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTtc                                                                                           >  5:401310/1‑138 (MQ=255)                                      aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                                                        <  8:88562/138‑1 (MQ=255)                                      aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                                                        >  7:232617/1‑138 (MQ=255)                                      aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                                                        <  6:318709/138‑1 (MQ=255)                                       aaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCaaa                                                                                      <  5:114376/139‑1 (MQ=255)                                            aagaatggAATCTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                                                 >  7:126220/7‑139 (MQ=255)                                            aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAACCTCTa                                                                                 >  5:97589/7‑139 (MQ=255)                                            aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                                                 >  1:227551/7‑139 (MQ=255)                                            aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                                                 >  7:350820/7‑139 (MQ=255)                                              gaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                                                 >  7:383888/5‑137 (MQ=255)                                                 tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                                                        <  3:318643/126‑1 (MQ=255)                                                 tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCATGGCGAGAAGCTCTTACCTGCAGCAATTTCTCa                                                                                        >  4:318643/2‑127 (MQ=255)                                                         cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc                                                                    >  3:293236/1‑139 (MQ=255)                                                         cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc                                                                    >  2:432906/1‑139 (MQ=255)                                                                   aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa                                                          <  2:66109/133‑1 (MQ=255)                                                                   aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa                                                          <  7:427466/133‑1 (MQ=255)                                                                            ttctcGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGAc                                                                 >  5:337077/2‑123 (MQ=255)                                                                            ttctcGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGAc                                                                 <  6:337077/122‑1 (MQ=255)                                                                                                cAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACATGGGAGAGGAGTGATTTTTCTTTCgcaat                             <  8:102138/139‑5 (MQ=255)                                                                                                                      cGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACATGGGAGAGGAGTGATTTTTCTTTCGCAATGGCTTCTTCTGTTTCCGGTGa        >  6:118927/1‑138 (MQ=255)                                                                                                                          gacaggAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACGCTATAGTTTTTCGTTCGACTGAAACATGGGAGAGGAGTGATTTTTCTTTCGCAATGGCTTCTTCTGTTTCCCGTGAAAGCg   <  5:436969/134‑1 (MQ=255)                                                                                                                            aaTGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACATGGGAGAGGAGTGATTTTTCTTTCGCAATGGCTTCTTCTGTTTCCGGTGAAAGCGt  <  5:118927/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    AATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACATGGGATAGGAGTGATTTTTCTTTCGTGATGGCTTCTTTTGTTTCTAGTGAAAGCGT  >  CP006881/2174155‑2174415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑