Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,851	T→C	E12E (GAA→GAG)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,851	0	T	C	100.0%	321.0 / NA	97	E12E (GAA→GAG)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (57/40);  total (57/40)

CTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATGTTTTCTTTTCCCCCGAGAAGTTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTT  >  CP006881/3722720‑3722984                                                                                                                                    |                                                                                                                                      ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATTAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt                                                                                                                                  >  8:438659/1‑137 (MQ=255) ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt                                                                                                                                  >  1:17223/1‑137 (MQ=255) ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt                                                                                                                                  >  5:123704/1‑137 (MQ=255) ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAAt                                                                                                                                  >  5:436735/1‑137 (MQ=255) ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg                                                                                                                                 <  8:7201/138‑1 (MQ=255) ctgAAAGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg                                                                                                                                 <  1:289299/138‑1 (MQ=255)    taaGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               <  6:337539/136‑1 (MQ=255)      aGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGt                                                                                                                           >  2:62029/1‑139 (MQ=255)      aGCCCCTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGt                                                                                                                           >  1:62523/1‑139 (MQ=255)        ccccTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTCAAAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGTCGtat                                                                                                                         >  2:217621/3‑137 (MQ=255)          gcTTTAACGCCATGAAGCTCTTCTACTTGTGCTTGATATATCTTTGATTCATCTTTCATCACTAAACTGAGTCTTGTTGCACAATGAGGCGCGATGATAATATTTTCTTTCGCTCCGAGAAGCTCAATGAGGCGTTTTg                                                                                                                       <  6:231691/138‑1 (MQ=255)          ccTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTTATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg                                                                                                                       <  7:438659/139‑1 (MQ=255)           cTTTAACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCGTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg                                                                                                                       >  3:200155/1‑138 (MQ=255)            tttaACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAACCTCAATGAGGCGTTTTGCa                                                                                                                     >  3:12222/1‑139 (MQ=255)              taaCGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAg                                                                                                                    <  2:41503/136‑1 (MQ=255)              taaCGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAg                                                                                                                    <  1:386146/136‑1 (MQ=255)               aaCGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   <  5:29979/137‑1 (MQ=255)                aCGCCATGAATCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACTATGAGCCGCGCTGATATTATTTTCTTTCCCTCCTAGTAGCTCAATGAGGCGTTTTGCAGtc                                                                                                                  <  7:277237/138‑1 (MQ=255)                aCGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGtc                                                                                                                  <  5:150514/138‑1 (MQ=255)                   ccATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGACAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGtc                                                                                                                  >  7:69536/1‑135 (MQ=255)                   ccATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGACAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGtc                                                                                                                  <  8:69536/135‑1 (MQ=255)                    cATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCttt                                                                                                             >  8:62140/1‑139 (MQ=255)                      tCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGAGAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGGTTTGCAGtctcgtggt                                                                                                           >  3:232753/1‑134 (MQ=255)                         aGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAAt                                                                                                        <  5:196138/139‑1 (MQ=255)                         aGCTCTTCTACTTGTGCCTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAAt                                                                                                        <  5:443479/139‑1 (MQ=255)                              ttctACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   <  3:334625/123‑1 (MQ=255)                              ttctACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   >  4:334625/1‑123 (MQ=255)                              ttctACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   <  5:186431/123‑1 (MQ=255)                              ttctACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   >  6:186431/1‑123 (MQ=255)                              ttctACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTATGTAATACATc                                                                                                   >  5:391544/1‑139 (MQ=255)                                       tgCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTcc                                                                                          <  2:282098/139‑1 (MQ=255)                                       tgCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTcc                                                                                          >  6:357539/1‑139 (MQ=255)                                        gCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   >  1:267830/1‑113 (MQ=255)                                        gCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGt                                                                                                                   <  2:267830/113‑1 (MQ=255)                                        gCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTccc                                                                                         >  3:168730/1‑139 (MQ=255)                                         cTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGATGATAATATTTTCTTTCCCTCCGAGAAGCAAAATGAGGCGTTTTGCAGTCTCTTTGTACTCAATCTAtttatacac                                                                                        >  4:301686/1‑133 (MQ=255)                                                       gATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAAc                                                                           >  4:262242/1‑138 (MQ=255)                                                       gATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATACATCTATTTCTCCCCCTTTTATTTAAAc                                                                           >  5:424665/1‑138 (MQ=255)                                                           cATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACaaaa                                                                       <  8:302670/138‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               >  2:443498/1‑81 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               >  7:407459/1‑81 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               >  7:413098/1‑81 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               <  1:443498/81‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               <  8:407459/81‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAg                                                                                                                               <  8:413098/81‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg                                                                                                                       <  8:56132/89‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTg                                                                                                                       >  7:56132/1‑89 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTtct                                                                                            >  2:277631/1‑116 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTtct                                                                                            <  1:277631/116‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTcc                                                                                          <  7:432305/118‑1 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACaaaaa                                                                      >  4:236766/1‑138 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACaaaaa                                                                      >  3:407019/1‑138 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTACTTAAACaaaa                                                                       >  3:416075/1‑137 (MQ=255)                                                            aTCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTATTTCCCTCCGAGAAGCTCATTGAGGCGTTTTGCAGTCTCTTTGTAATCAATCTAATTATCACCCTTTGATTTAAACaaaaa                                                                      >  6:1554/1‑138 (MQ=255)                                                            aTCTTACATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTcc                                                                                          >  8:432305/1‑118 (MQ=255)                                                                  catcaCTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCt                                                                <  7:243702/138‑1 (MQ=255)                                                                           aCGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGcc                                                      <  2:62523/139‑1 (MQ=255)                                                                            cGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCa                                                     <  4:194063/139‑1 (MQ=255)                                                                            cGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCa                                                     <  1:62029/139‑1 (MQ=255)                                                                               aGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg                                                                                                                                 >  2:420128/1‑60 (MQ=255)                                                                               aGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATg                                                                                                                                 <  1:420128/60‑1 (MQ=255)                                                                               aGTCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCAACTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAAc                                                   >  7:41929/1‑138 (MQ=255)                                                                                 tCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGaa                                                >  7:127914/1‑139 (MQ=255)                                                                                 tCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGaa                                                >  8:400635/1‑139 (MQ=255)                                                                                 tCTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGGAATCCATCTATTTCTCCCCGTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGaa                                                >  4:292307/1‑139 (MQ=255)                                                                                 tCTTGTTGCACAATGAGCCGCGATGATAATATTTTCTTTCACTACGTGACGCTCAATGAGGAGTTTTGCAGTCTCATTGTAATCACTCTAATTCT‑ACGCTATAATTTAAACAAAAAAGACCTAAAAATCGCCaaacaa                                                >  2:438698/1‑134 (MQ=255)                                                                                  cTTGTTGCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGaa                                                >  4:282326/1‑138 (MQ=255)                                                                                      ttgCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAAt                                                                                                        >  2:58777/1‑78 (MQ=255)                                                                                      ttgCACAATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAAt                                                                                                        <  1:58777/78‑1 (MQ=255)                                                                                         cacaATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGa                                         >  6:166161/1‑138 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCtt                                                                                     <  4:252519/92‑1 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCtt                                                                                     >  3:252519/1‑92 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATa                                              <  2:387550/131‑1 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATa                                              >  1:387550/1‑131 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATa                                              <  4:126723/131‑1 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATa                                              >  3:126723/1‑131 (MQ=255)                                                                                           caATGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGacac                                      >  8:434126/1‑139 (MQ=255)                                                                                              tGAGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGc                                   >  3:215762/1‑139 (MQ=255)                                                                                                aGCCGCGCTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTACTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCg                                  <  4:416075/138‑1 (MQ=255)                                                                                                     cgcTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCtt                            >  2:7641/1‑139 (MQ=255)                                                                                                     cgcTGATAATATTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATACATCTATTTCTACCACTTTTATTTAAACAAAAAAGACATAAAATTCGCCAAAGAATAAATGACACAGCGATCCtt                            >  2:469/1‑139 (MQ=255)                                                                                                          ataataTTTTCTTTCCCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCa                       >  6:427184/1‑139 (MQ=255)                                                                                                                ttttctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACg                 <  1:134349/139‑1 (MQ=255)                                                                                                                ttttctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACg                 <  6:153912/139‑1 (MQ=255)                                                                                                                ttttctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACg                 <  1:122824/139‑1 (MQ=255)                                                                                                                  ttctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACg                 >  4:286998/1‑137 (MQ=255)                                                                                                                  ttctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACg                 <  3:286998/137‑1 (MQ=255)                                                                                                                   tctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCa               <  5:1554/138‑1 (MQ=255)                                                                                                                    ctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCaaa             >  8:441160/1‑139 (MQ=255)                                                                                                                    ctttccCTCCGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCaaa             >  5:316820/1‑139 (MQ=255)                                                                                                                      ttccctccGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAATCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAAtt           >  4:16689/7‑139 (MQ=255)                                                                                                                      ttccctccGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAAtt           >  7:106376/7‑139 (MQ=255)                                                                                                                        ccctccGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTATTCATTTACGCAAAttt          >  2:283426/5‑138 (MQ=255)                                                                                                                        ccctccGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCAACTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAAttt          <  8:41929/134‑1 (MQ=255)                                                                                                                          ctccGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAg       >  7:119163/3‑139 (MQ=255)                                                                                                                             cGAGAAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTATTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTc    >  8:197007/1‑139 (MQ=255)                                                                                                                               agaAGCTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCCTGTATTTCTCCCCCTTTTATTTAATCAAAAAACACCTAAAATTAGCCAACGAAAAAAGGACACACCCATCCTTTTTCATTTACGGAAATTTTATGTCtt  >  5:292283/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      CTGAAAGCGCCTTTTACGCCATCAAGCTCTTCTACTTGTGCTTGATCTATCTTTGATTCATCTTTCATCACTAAACGCAGTCTTGTTGCACAATGAGCCGCGCTGATAATGTTTTCTTTTCCCCCGAGAAGTTCAATGAGGCGTTTTGCAGTCTCTTTGTAATCCATCTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTT  >  CP006881/3722720‑3722984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑