Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,022	G→A	intergenic (‑136/‑165)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,022	0	G	A	99.0%	304.6 / NA	98	intergenic (‑136/‑165)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (35/62);  minor base T (0/1);  total (35/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTT‑TTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722891‑3723156                                                                                                                                    |                                                                                                                                         ttCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATa                                                                                                                                    <  5:70480/138‑1 (MQ=255)   ctcCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACg                                                                                                                                  <  5:166161/138‑1 (MQ=255)     cccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCGTATTCAGATTAAGAATACGCtt                                                                                                                               >  2:180355/1‑139 (MQ=255)      ccccTTTTATTTAAACAAAAAATACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTTCTTTACAAGTCAAAATCACGCTTATTCAGATTAAGAATACGCtt                                                                                                                               >  5:430429/1‑138 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              <  3:192154/139‑1 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              <  3:185379/139‑1 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              <  5:59236/139‑1 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              >  2:53751/1‑139 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              <  2:387664/139‑1 (MQ=255)      ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGAttt                                                                                                                              >  7:317587/1‑139 (MQ=255)       cccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtc                                                                                                                             <  8:119163/139‑1 (MQ=255)       cccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtc                                                                                                                             <  4:193526/139‑1 (MQ=255)         cTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcat                                                                                                                           <  7:62140/139‑1 (MQ=255)          ttttATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcat                                                                                                                           >  6:65466/1‑138 (MQ=255)           tttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatc                                                                                                                          >  7:438884/1‑138 (MQ=255)            ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  8:317587/138‑1 (MQ=255)            ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  1:177480/138‑1 (MQ=255)              atttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATa                                                                                                                       <  5:65466/138‑1 (MQ=255)                 taAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTTCCTGCTTTACCAGTCACAATCCCGATTATTCAGATTAAGAATACGCTTTCATCATAAAt                                                                                                                    <  1:283426/138‑1 (MQ=255)                  aaaCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAAtca                                                                                                                  >  5:88901/1‑137 (MQ=255)                    aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                 >  8:366537/1‑138 (MQ=255)                                       cGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACta                                                                                             >  5:444067/1‑138 (MQ=255)                                             cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttt‑tt                                                                                     <  6:424665/139‑1 (MQ=255)                                                    aGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTa                                                                                >  4:141426/1‑138 (MQ=255)                                                     ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTaaa                                                                              <  6:444067/139‑1 (MQ=255)                                                        cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAg                                                                           >  1:153248/1‑139 (MQ=255)                                                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAAt                                                                                                                    >  1:80140/1‑98 (MQ=255)                                                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAAt                                                                                                                    <  2:80140/98‑1 (MQ=255)                                                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAATATTTTT‑TTAGTTAAATAGt                                                                          >  2:192440/1‑139 (MQ=255)                                                          caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGtt                                                                         >  1:324704/1‑139 (MQ=255)                                                          caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGtt                                                                         <  7:366537/139‑1 (MQ=255)                                                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATa                                                                                                                       <  6:439682/93‑1 (MQ=255)                                                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATa                                                                                                                       >  5:439682/1‑93 (MQ=255)                                                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGttt                                                                        >  1:414461/1‑139 (MQ=255)                                                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTATTTAATTAGTTTgttt                                                                    >  2:320050/1‑135 (MQ=255)                                                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATATTTTGATa                                                                    >  4:44922/1‑138 (MQ=255)                                                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTGATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATATTTTGATa                                                                    >  3:244469/1‑138 (MQ=255)                                                                  cTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATata                                                                  <  7:434126/138‑3 (MQ=255)                                                                  cTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATat                                                                   <  6:430429/137‑2 (MQ=255)                                                                     tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAAcat                                                              >  1:112077/1‑137 (MQ=255)                                                                     tttCATTTAATCAAATTTTAGGTCTTGCCTGCTTTACAAGTCACAATCCCGCATATTCAGATTAAGAATACGCTTTCATCATTAATCATGATAGCGTTTTCGTCAACG‑ATTTTATTAGTTAAATAGTTTTATataaaat                                                              >  2:149589/1‑136 (MQ=255)                                                                            tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTaaa                                                                              >  4:111309/1‑116 (MQ=39)                                                                            tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTaaa                                                                              <  3:111309/116‑1 (MQ=39)                                                                            tACGCAAATTTTAGGTCTTGCATGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGAc                                                        >  4:266604/1‑138 (MQ=255)                                                                             aCGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGCCa                                                       >  8:289737/1‑138 (MQ=255)                                                                               gCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACaaaa                                                    <  1:193760/139‑1 (MQ=255)                                                                                       tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                             <  1:40049/138‑1 (MQ=255)                                                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGt                                                                          <  7:314809/103‑1 (MQ=255)                                                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGt                                                                          >  8:314809/1‑103 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCTCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     <  3:266604/138‑2 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                    >  8:261952/1‑137 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     <  8:106376/138‑2 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     <  4:215762/138‑2 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     <  8:127914/138‑2 (MQ=255)                                                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAATTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     <  4:352635/138‑2 (MQ=255)                                                                                                       tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                             <  2:112077/138‑1 (MQ=255)                                                                                                         ccATTCACAATCGCGCTTATTGAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACATAAATTCGTAAAAATTAATTGTGaaata                           <  1:438698/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  3:141426/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  3:292307/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  2:414461/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  3:316245/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  3:44922/138‑3 (MQ=255)                                                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  6:436735/138‑3 (MQ=255)                                                                                                          cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                         <  4:407019/139‑1 (MQ=255)                                                                                                             tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      <  6:88901/139‑1 (MQ=255)                                                                                                             tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      <  7:77744/139‑1 (MQ=255)                                                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      >  7:33837/1‑138 (MQ=255)                                                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGCGaaata                           <  6:68467/133‑3 (MQ=255)                                                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGCGaaata                           >  5:68467/1‑131 (MQ=255)                                                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTAATCATAAATCATGATAGAGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTATGATATAACATTTATAAAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      >  4:202416/1‑138 (MQ=255)                                                                                                               acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                    <  8:438884/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCCTAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:292283/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:392756/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:98326/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  1:149589/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:289737/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  1:180355/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:344148/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  3:282326/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTAATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  5:13056/139‑1 (MQ=255)                                                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCGTTCATCCTAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  4:232753/139‑1 (MQ=255)                                                                                                                     ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc               <  1:64552/138‑1 (MQ=255)                                                                                                                     ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc               <  3:16689/138‑1 (MQ=255)                                                                                                                       gCTTATTCATATTTAGAATACTCTTTCATCATAAATCATGATATCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  7:40296/139‑1 (MQ=255)                                                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGt                                                                          >  6:379617/1‑77 (MQ=255)                                                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGt                                                                          <  5:379617/77‑1 (MQ=255)                                                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  7:261952/139‑1 (MQ=255)                                                                                                                       gCTTATTCAGACTAAGACTACTCATTCATCATAAATCAAGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGGATGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  1:300719/139‑1 (MQ=255)                                                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTTTGAAATACTTCACAATATCGTGc           >  5:318097/1‑139 (MQ=255)                                                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           >  5:228078/1‑121 (MQ=255)                                                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  6:228078/123‑3 (MQ=255)                                                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc           >  6:43674/1‑139 (MQ=255)                                                                                                                           aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  8:33837/137‑1 (MQ=255)                                                                                                                           aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  1:53751/137‑1 (MQ=255)                                                                                                                               aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt     <  7:320852/138‑1 (MQ=255)                                                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  5:52676/1‑124 (MQ=255)                                                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:52676/124‑1 (MQ=255)                                                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg  >  2:305711/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                         TTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTT‑TTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722891‑3723156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑