Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,025 A→G L48L (CTT→CTC)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0250AG97.9% 147.0 / NA 48L48L (CTT→CTC) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (10/37);  minor base C (1/0);  total (11/37)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAA  >  CP006881/3724893‑3725147
                                                                                                                                    |                                                                                                                          
aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAACCGAAACGAGTcct                                                                                                                      <  1:50115/139‑2 (MQ=255)
aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct                                                                                                                      <  1:50060/139‑2 (MQ=255)
   ctggCCCGCTCTCTCCAGCTTTGGCGATTCCTTGAAGTCTTGCAAACATCTTCTTTTATAAAGCGAGGCGGATTTCGCTTTCTGCTTTTCTTCCTCATTCACATCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                   <  7:19631/137‑1 (MQ=255)
   cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTTCTGCGTTTCTTCCGCATTCACCGCCCTCCGCCCTTCATAAAACGAAACCAGTCCcgcg                                                                                                                   >  2:80649/1‑139 (MQ=255)
     ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                 >  1:46075/1‑139 (MQ=255)
           ctctctCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAc                                                                                                           >  4:339498/1‑139 (MQ=255)
                tcCTGCTTTGGCGATGCCTTGAAGGCTTGCAAACATCTTATTTTATAAAGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                        <  4:62862/137‑1 (MQ=255)
                  cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                    <  3:318667/139‑2 (MQ=255)
                  cTGCTTTGCCTATTCCTTGAAGGCTTGCAACAAGCTTCTTTAATAACCCGCGGCGGCTTTCGCTTTCTGCTTTGCTTCCTCATTCACAGCAAACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                    <  4:273104/139‑2 (MQ=255)
                     cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTACGCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgccatt                                                                                                 >  6:221007/1‑135 (MQ=255)
                        tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                              >  5:118329/1‑139 (MQ=255)
                        tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                               <  7:74978/138‑1 (MQ=255)
                        tGGCGATGCCTTGACGGCTTGCAAACATCTACTTTTATAAACCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                               <  2:148377/138‑1 (MQ=255)
                         ggCGTTGCCTTGAAGGCTTGATAACAGCTTCTTTTTTAAAGCGCGGGGGCTTTCGCTTGCTGCTTTTCTTCCTCATTCACAGCCATCCGCCTTTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                             <  6:252377/139‑1 (MQ=255)
                          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCTGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                             <  4:225739/138‑1 (MQ=255)
                          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                             <  1:80649/138‑1 (MQ=255)
                          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                             <  5:413195/138‑1 (MQ=255)
                          gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTCTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                             <  4:139964/138‑1 (MQ=255)
                              ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                        <  7:413749/139‑1 (MQ=255)
                                     aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                 <  3:410875/139‑1 (MQ=255)
                                      aGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                 <  7:410671/138‑1 (MQ=255)
                                        gCTTGCAAACATCTTCTTTTATATAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                              <  3:124895/139‑1 (MQ=255)
                                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                              <  3:339498/139‑1 (MQ=255)
                                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                              <  6:118329/139‑1 (MQ=255)
                                        gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                              <  8:210606/139‑1 (MQ=255)
                                        gCTTGCAAACATATTATTTTATAATGGGCGGCGGCTTTCGCTTTCTGCTTTTCTACCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                              <  1:358424/139‑1 (MQ=255)
                                           tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTAACAGCCATCCGCCATTGATAAAACGAAACGAGTCCTGCCGCCTGCACAGCCGCCATTAATTTGTTAGTTCGCTGAGCCg                                                                           >  6:393379/1‑139 (MQ=255)
                                             cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                         <  8:312659/139‑1 (MQ=255)
                                               aaCATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATa                                                                       <  6:221623/139‑1 (MQ=255)
                                                             taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                         <  4:178588/139‑1 (MQ=255)
                                                                       cggcTTTAGCTTTCTGGTTTTCTTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCGTTAGTTGGTTTGTACGCGGAGCCGAATATACCCCCGCAGCCGCACgttttgt                                               >  2:295900/1‑137 (MQ=255)
                                                                          cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATGCATAATCCGTAACGAGTCCTGCGGCCTGCACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                            <  7:343054/139‑1 (MQ=255)
                                                                          cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAGCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                            <  7:99273/139‑1 (MQ=255)
                                                                          cTTTCGCTTTCTGCTTTTCTTCCACATTCACAGCACACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGATTAGACCGCGGCAGCCGCAGTTTTTCTGTc                                            <  1:207699/139‑1 (MQ=255)
                                                                               gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                       <  8:332231/139‑1 (MQ=255)
                                                                                       cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTTGTATTTAACCTGAGCCCCATATACCCCGGCCGCCGCCCTTTTTATGTCCGGCGTCACCCAg                               >  8:294321/2‑139 (MQ=255)
                                                                                                   aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAg                   <  5:334927/139‑1 (MQ=255)
                                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                  <  6:40467/139‑1 (MQ=255)
                                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                  <  3:24595/139‑1 (MQ=255)
                                                                                                    ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                  <  1:164130/139‑1 (MQ=255)
                                                                                                      cacaCCCAGCCGCATTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                 <  1:285806/138‑1 (MQ=255)
                                                                                                         aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTGCCCCAGTTCATAAAAAAGACCCTc             >  3:113704/1‑139 (MQ=255)
                                                                                                           ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca           <  7:404456/139‑1 (MQ=255)
                                                                                                           ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca           <  5:221007/139‑1 (MQ=255)
                                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa   <  7:228464/136‑1 (MQ=255)
                                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa   <  7:294321/136‑1 (MQ=255)
                                                                                                                   cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGGATGTACGCTGAGCCGAATAGACCGCGGCCGGCCCAGTTTTTCTGTCCGGCGTCACCCAGTTAATGAAAAAGACCAACCCCACCCGt    >  2:87557/4‑138 (MQ=255)
                                                                                                                     ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTTGTATGTAAGCGGAGCCGAATATACCGCGGCAGCCGCAGTTTTTATTTCCGGGGTCACCCAGTTAATCAAAAAGACCATCCACACCCGTaa  >  8:2398/2‑138 (MQ=255)
                                                                                                                                    |                                                                                                                          
ATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAA  >  CP006881/3724893‑3725147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑