Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,043	C→A	A42A (GCG→GCT)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,043	0	C	A	100.0%	118.4 / NA	39	A42A (GCG→GCT)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/32);  total (7/32)

TCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAA  >  CP006881/3724909‑3725174                                                                                                                                       |                                                                                                                                    tcCTGCTTTGGCGATGCCTTGAAGGCTTGCAAACATCTTATTTTATAAAGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                                                   <  4:62862/137‑1 (MQ=255)   cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                               <  3:318667/139‑2 (MQ=255)   cTGCTTTGCCTATTCCTTGAAGGCTTGCAACAAGCTTCTTTAATAACCCGCGGCGGCTTTCGCTTTCTGCTTTGCTTCCTCATTCACAGCAAACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                               <  4:273104/139‑2 (MQ=255)      cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTACGCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgccatt                                                                                                                            >  6:221007/1‑135 (MQ=255)         tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                                         >  5:118329/1‑139 (MQ=255)         tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                          <  7:74978/138‑1 (MQ=255)         tGGCGATGCCTTGACGGCTTGCAAACATCTACTTTTATAAACCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                          <  2:148377/138‑1 (MQ=255)          ggCGTTGCCTTGAAGGCTTGATAACAGCTTCTTTTTTAAAGCGCGGGGGCTTTCGCTTGCTGCTTTTCTTCCTCATTCACAGCCATCCGCCTTTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  6:252377/139‑1 (MQ=255)           gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCTGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  4:225739/138‑1 (MQ=255)           gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  5:413195/138‑1 (MQ=255)           gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  1:80649/138‑1 (MQ=255)           gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTCTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                        <  4:139964/138‑1 (MQ=255)               ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                   <  7:413749/139‑1 (MQ=255)                      aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                            <  3:410875/139‑1 (MQ=255)                       aGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                            <  7:410671/138‑1 (MQ=255)                         gCTTGCAAACATCTTCTTTTATATAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  3:124895/139‑1 (MQ=255)                         gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  6:118329/139‑1 (MQ=255)                         gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  3:339498/139‑1 (MQ=255)                         gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  8:210606/139‑1 (MQ=255)                         gCTTGCAAACATATTATTTTATAATGGGCGGCGGCTTTCGCTTTCTGCTTTTCTACCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                         <  1:358424/139‑1 (MQ=255)                            tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTAACAGCCATCCGCCATTGATAAAACGAAACGAGTCCTGCCGCCTGCACAGCCGCCATTAATTTGTTAGTTCGCTGAGCCg                                                                                                      >  6:393379/1‑139 (MQ=255)                              cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                    <  8:312659/139‑1 (MQ=255)                                aaCATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATa                                                                                                  <  6:221623/139‑1 (MQ=255)                                              taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                    <  4:178588/139‑1 (MQ=255)                                                        cggcTTTAGCTTTCTGGTTTTCTTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCGTTAGTTGGTTTGTACGCGGAGCCGAATATACCCCCGCAGCCGCACgttttgt                                                                          >  2:295900/1‑137 (MQ=255)                                                           cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATGCATAATCCGTAACGAGTCCTGCGGCCTGCACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  7:343054/139‑1 (MQ=255)                                                           cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAGCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  7:99273/139‑1 (MQ=255)                                                           cTTTCGCTTTCTGCTTTTCTTCCACATTCACAGCACACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGATTAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                       <  1:207699/139‑1 (MQ=255)                                                                gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                  <  8:332231/139‑1 (MQ=255)                                                                        cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTTGTATTTAACCTGAGCCCCATATACCCCGGCCGCCGCCCTTTTTATGTCCGGCGTCACCCAg                                                          >  8:294321/2‑139 (MQ=255)                                                                                    aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAg                                              <  5:334927/139‑1 (MQ=255)                                                                                     ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  6:40467/139‑1 (MQ=255)                                                                                     ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  3:24595/139‑1 (MQ=255)                                                                                     ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                             <  1:164130/139‑1 (MQ=255)                                                                                       cacaCCCAGCCGCATTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                                            <  1:285806/138‑1 (MQ=255)                                                                                          aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTGCCCCAGTTCATAAAAAAGACCCTc                                        >  3:113704/1‑139 (MQ=255)                                                                                            ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                      <  7:404456/139‑1 (MQ=255)                                                                                            ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                      <  5:221007/139‑1 (MQ=255)                                                                                                    cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                              <  7:228464/136‑1 (MQ=255)                                                                                                    cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                              <  7:294321/136‑1 (MQ=255)                                                                                                    cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGGATGTACGCTGAGCCGAATAGACCGCGGCCGGCCCAGTTTTTCTGTCCGGCGTCACCCAGTTAATGAAAAAGACCAACCCCACCCGt                               >  2:87557/4‑138 (MQ=255)                                                                                                      ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTTGTATGTAAGCGGAGCCGAATATACCGCGGCAGCCGCAGTTTTTATTTCCGGGGTCACCCAGTTAATCAAAAAGACCATCCACACCCGTaa                             >  8:2398/2‑138 (MQ=255)                                                                                                                                ccTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaa  <  1:295900/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAA  >  CP006881/3724909‑3725174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑