Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,082	A→C	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	135.8 / NA	42	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/24);  total (18/24)

TAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAG  >  CP006881/3724954‑3725214                                                                                                                                 |                                                                                                                                      taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                                                             <  4:178588/139‑1 (MQ=255)           cggcTTTAGCTTTCTGGTTTTCTTGCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCCCAGCCGCCGTTAGTTGGTTTGTACGCGGAGCCGAATATACCCCCGCAGCCGCACgttttgt                                                                                                                   >  2:295900/1‑137 (MQ=255)              cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATGCATAATCCGTAACGAGTCCTGCGGCCTGCACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                                <  7:343054/139‑1 (MQ=255)              cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCAGCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                                <  7:99273/139‑1 (MQ=255)              cTTTCGCTTTCTGCTTTTCTTCCACATTCACAGCACACCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGATTAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                                <  1:207699/139‑1 (MQ=255)                   gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                                                           <  8:332231/139‑1 (MQ=255)                           cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTTGTATTTAACCTGAGCCCCATATACCCCGGCCGCCGCCCTTTTTATGTCCGGCGTCACCCAg                                                                                                   >  8:294321/2‑139 (MQ=255)                                       aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAg                                                                                       <  5:334927/139‑1 (MQ=255)                                        ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                      <  1:164130/139‑1 (MQ=255)                                        ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                      <  6:40467/139‑1 (MQ=255)                                        ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                      <  3:24595/139‑1 (MQ=255)                                          cacaCCCAGCCGCATTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGAc                                                                                     <  1:285806/138‑1 (MQ=255)                                             aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGCGCCGAATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTGCCCCAGTTCATAAAAAAGACCCTc                                                                                 >  3:113704/1‑139 (MQ=255)                                               ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  7:404456/139‑1 (MQ=255)                                               ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  5:221007/139‑1 (MQ=255)                                                       cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                       <  7:294321/136‑1 (MQ=255)                                                       cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                       <  7:228464/136‑1 (MQ=255)                                                       cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGGATGTACGCTGAGCCGAATAGACCGCGGCCGGCCCAGTTTTTCTGTCCGGCGTCACCCAGTTAATGAAAAAGACCAACCCCACCCGt                                                                        >  2:87557/4‑138 (MQ=255)                                                         ttCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTGTTTTGTATGTAAGCGGAGCCGAATATACCGCGGCAGCCGCAGTTTTTATTTCCGGGGTCACCCAGTTAATCAAAAAGACCATCCACACCCGTaa                                                                      >  8:2398/2‑138 (MQ=255)                                                                                   ccTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaa                                           <  1:295900/139‑1 (MQ=255)                                                                                                          tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggc                    >  1:105701/1‑139 (MQ=255)                                                                                                            tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                                                                      >  5:59063/1‑55 (MQ=255)                                                                                                            tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                                                                      <  6:59063/55‑1 (MQ=255)                                                                                                            tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcag                  >  8:261366/1‑139 (MQ=255)                                                                                                              tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                                                                <  3:344113/59‑1 (MQ=255)                                                                                                              tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                                                                >  4:344113/1‑59 (MQ=255)                                                                                                              tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACAcc                                                                           >  4:44819/1‑80 (MQ=255)                                                                                                              tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACAcc                                                                           <  3:44819/80‑1 (MQ=255)                                                                                                              tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc                >  8:266580/1‑139 (MQ=255)                                                                                                                cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  2:105701/138‑1 (MQ=255)                                                                                                                cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  4:113704/138‑1 (MQ=255)                                                                                                                   tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑t          >  5:156798/1‑139 (MQ=255)                                                                                                                    gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt                                                    <  7:260046/97‑1 (MQ=255)                                                                                                                    gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt                                                    >  8:260046/1‑97 (MQ=255)                                                                                                                    gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCAGCC‑tt         >  3:432265/1‑139 (MQ=255)                                                                                                                    gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑tt         >  1:35968/1‑139 (MQ=255)                                                                                                                     aGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGCCCATCCACACCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑ttt        >  3:154387/1‑139 (MQ=255)                                                                                                                          aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               >  2:181679/1‑128 (MQ=255)                                                                                                                          aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  1:181679/128‑1 (MQ=255)                                                                                                                           aTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa                                          >  6:215093/1‑100 (MQ=255)                                                                                                                           aTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa                                          <  5:215093/100‑1 (MQ=255)                                                                                                                            tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTagcag  <  3:49153/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAG  >  CP006881/3724954‑3725214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑