Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,970,967 G→T L192I (CTT→ATT)  U712_20470 ← Sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,970,9670GT96.4% 162.4 / NA 56L192I (CTT→ATT) U712_20470Sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (21/33);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGC  >  CP006881/3970834‑3971093
                                                                                                                                     |                                                                                                                              
tcatcaTGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGcc                                                                                                                            >  1:425530/1‑138 (MQ=255)
 catcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCa                                                                                                                           <  4:16128/138‑1 (MQ=255)
   tcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAAc                                                                                                                        <  4:209871/139‑1 (MQ=255)
     atGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCt                                                                                                                      >  5:189690/1‑139 (MQ=255)
        tCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCtgtg                                                                                                                   >  7:341656/1‑139 (MQ=255)
        tCCATAC‑TTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGc                                                                                                                  >  3:410524/1‑139 (MQ=255)
          cATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGc                                                                                                                  <  2:232439/138‑1 (MQ=255)
          cATAC‑TTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg                                                                                                                <  4:410524/139‑1 (MQ=255)
           aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg                                                                                                                <  4:310514/139‑1 (MQ=255)
           aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg                                                                                                                <  3:75294/139‑1 (MQ=255)
           aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg                                                                                                                <  5:390499/139‑1 (MQ=255)
           aTACTTTTTAACCTTCCTAGAGAAATTCCCTTTCGCAATCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg                                                                                                                <  6:44999/139‑1 (MQ=255)
             aCTTTTTAACCTTCCGCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGa                                                                                                              <  8:189391/139‑1 (MQ=255)
              cTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGa                                                                                                              <  2:425530/138‑1 (MQ=255)
                 tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc                                                                                                           >  8:350892/1‑138 (MQ=255)
                 tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc                                                                                                           <  4:153197/138‑1 (MQ=255)
                 tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGAAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGcc                                                                                                          >  4:142001/1‑139 (MQ=255)
                   tAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAg                                                                                                        >  4:175675/1‑139 (MQ=255)
                     aCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa                                                                                                      <  2:157211/139‑1 (MQ=255)
                         tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt                                                                                                  >  2:57489/1‑139 (MQ=255)
                         tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt                                                                                                  >  1:392645/1‑139 (MQ=255)
                           cTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGTGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgtg                                                                                                 <  7:424366/138‑1 (MQ=255)
                                           tCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCAtt                                                                                  <  4:18624/137‑1 (MQ=255)
                                           tCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCAtt                                                                                  <  4:341125/137‑1 (MQ=255)
                                               aaGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa                                                                            <  2:392645/139‑1 (MQ=255)
                                                 gCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGTGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa                                                                            <  1:397835/137‑1 (MQ=255)
                                                  cTCCATCGGCTGGTTTAGCATATCTGAAAGAGGGTGGGTAATGGTTCTTGCAAGAAAACTACCTAGAAGAGCCGTCAAAACAATTGCGAAACTTGGGGCGGTCGCGAGTATCGTGTTGATCGTCTTCATTTGATTaatg                                                                         <  6:311698/139‑3 (MQ=255)
                                                  cTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAg                                                                         <  8:365670/139‑1 (MQ=255)
                                                    ccATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc                                                                       <  4:111855/139‑1 (MQ=255)
                                                    ccATCGCCTGCTTTCGCATATCTCAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc                                                                       <  4:392742/139‑1 (MQ=255)
                                                     cATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc                                                                       <  3:175675/138‑1 (MQ=255)
                                                                 tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt                                                           <  8:341656/138‑1 (MQ=255)
                                                                 tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt                                                           <  7:6982/138‑1 (MQ=255)
                                                                    acagtCTGAAAGCGGGTGTGTAATGGTTCTTTCCAGAAAAATACCGAGAAGAGCCGTCAAAAAAATTGCCAAACCTGTGCCGGACGCCATTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg                                                       <  6:336552/135‑1 (MQ=255)
                                                                        tCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACaa                                                   >  1:46268/1‑139 (MQ=255)
                                                                                cGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc                                           <  8:323637/139‑1 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt                                                                     <  4:330757/110‑1 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt                                                                     >  3:330757/1‑110 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATcgcg                                        <  7:230145/139‑1 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATATTCCATACTCGCGACAACGTAGATctg                                         >  5:336552/1‑136 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAAAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa                                                                            >  3:294623/1‑103 (MQ=255)
                                                                                   gtgggtAATGGTTCTTGCCAGAAAAATACCGAAAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa                                                                            <  4:294623/103‑1 (MQ=255)
                                                                                                cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa                                                                                             >  6:267537/1‑73 (MQ=255)
                                                                                                cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa                                                                                             <  5:267537/73‑1 (MQ=255)
                                                                                                cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt                                                           >  3:150761/1‑107 (MQ=255)
                                                                                                cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt                                                           <  4:150761/107‑1 (MQ=255)
                                                                                                cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACACCCTCtt                           >  2:357804/1‑139 (MQ=255)
                                                                                                  tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTgg                         >  6:241618/1‑139 (MQ=255)
                                                                                                  tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTgg                         >  3:388660/1‑139 (MQ=255)
                                                                                                    ccAGAAACATCCCGTGGATGGCCGTCAAAACAATTGCCAAACGTGGGCGGGACGCCAGTATCGTGTTTTTCGTCTTCATTTTATTATAGACATCTTCCATAGTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtt                       <  2:422600/139‑1 (MQ=255)
                                                                                                      aGAAAAATACCGAGACGAGCCGTCAAAACAATTGCCAAACCTGGGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt                     <  5:51898/139‑1 (MQ=255)
                                                                                                      aGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt                     <  5:387604/139‑1 (MQ=255)
                                                                                                        aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCCCAACCTCTTGGtttt                     >  8:267705/1‑137 (MQ=255)
                                                                                                        aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt                     >  7:216029/1‑137 (MQ=255)
                                                                                                                  agaagaGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCGTCATTTGATTAAAGAAATCTTACATACTCGCGACCACCGAGATCGCGCCCACACCCGCGTTGTTTTCAGTCAATACgg         >  2:162561/1‑139 (MQ=255)
                                                                                                                         ccGTCAAAACAATTTCCAAACCTGTGCCGGACGCCAGTATCGTGTTTATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTATTGGTTTTCAGTCATGACGGGCTTcgc  >  4:166156/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                              
TCATCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGC  >  CP006881/3970834‑3971093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑