Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,174,581 A→G I115I (ATT→ATC)  U712_10875 ← CCA‑adding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,174,5810AG100.0% 153.2 / NA 48I115I (ATT→ATC) U712_10875CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)

CTCTCAACATACGAAGTGCATCCTCTTGAAATCTGTCCTCAGGCTTTCCGACGGTTCGAATCAGTTTCTGATCAATATCTTTCTTGCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGC  >  CP006881/2174445‑2174713
                                                                                                                                        |                                                                                                                                    
ctctCAGCCTACGAAGCGCATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGAt                                                                                                                                    <  6:29681/139‑1 (MQ=255)
   tcAGCATACGAAGCGCATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGt                                                                                                                                 >  7:421926/1‑139 (MQ=255)
          aCGAAGCGCATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCCCCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAAtct                                                                                                                          >  3:425963/1‑139 (MQ=255)
          aCGAAGAGCATCCTCTTGAAATCTATCCTCAGGCTTTACAACGGTTCGATTCAATTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGACCGCTGTCATTGCCATCGCGTTGATCGTTAAAtct                                                                                                                          >  1:74929/1‑139 (MQ=255)
                 gCATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGtt                                                                                                                   >  5:238198/1‑139 (MQ=255)
                 gCATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCAGCGtt                                                                                                                   >  3:375124/1‑139 (MQ=255)
                  cATCCTCTTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGttt                                                                                                                  >  4:262654/1‑139 (MQ=255)
                      ctctTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTaga                                                                                                              <  8:143146/139‑3 (MQ=255)
                      ctctTGAAATCTATCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTaga                                                                                                              <  2:60865/139‑3 (MQ=255)
                                 tatCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGAt                                                                                                                                    >  5:267376/3‑106 (MQ=255)
                                 tatCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGAt                                                                                                                                    <  6:267376/104‑1 (MQ=255)
                                 tatCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTaa                                                                                                   >  5:338009/3‑139 (MQ=255)
                                 tatCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTaa                                                                                                   >  5:404332/3‑139 (MQ=255)
                                 tatCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTATCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGATGTCATGGCCATCGCATTGATCGTTAAATATCTGCGTGTTAGATCCTCTTCTa                                                                                                    >  1:199537/3‑138 (MQ=255)
                                   tCCTCAGGCTTTCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAat                                                                                                  >  4:75016/1‑138 (MQ=255)
                                             ttCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATaaa                                                                                       >  3:161212/1‑139 (MQ=255)
                                               ccaaCGGTTCGAATCACTTTCTGATCAATATCATTCTTGCAGCCAAAATAATCAAGCACCTTCCCCTCCGCTCTCAGTGCCATCCAAGTGATCGTTAAATCTATGCGTTTTAGATCCTCTTCTAATGATTAGATATAtt                                                                                     >  6:370323/4‑139 (MQ=255)
                                                  aCGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCa                                                                                  <  8:108134/139‑1 (MQ=255)
                                                  aCGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCa                                                                                  <  1:277787/139‑1 (MQ=255)
                                                      ttCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGATTTATATACTCTTCTAATTATGAGATAAATTGAACTTc                                                                              >  1:173784/1‑139 (MQ=255)
                                                         gAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGa                                                                           <  4:161212/139‑1 (MQ=255)
                                                                tttCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGtta                                                                                                                               <  2:195924/80‑3 (MQ=255)
                                                                tttCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGtta                                                                                                                               >  1:195924/1‑78 (MQ=255)
                                                                tttCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTTCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGtctt                                                                    >  3:257117/1‑139 (MQ=255)
                                                                     gATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGtt                                                                                                                                >  1:178692/1‑73 (MQ=255)
                                                                     gATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGtt                                                                                                                                <  2:178692/74‑2 (MQ=255)
                                                                      aTCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGtcttctgaaa                                                              <  3:75016/139‑5 (MQ=255)
                                                                          atatCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATc                                                                                                            <  5:421419/89‑1 (MQ=255)
                                                                          atatCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATc                                                                                                            >  6:421419/1‑89 (MQ=255)
                                                                             tctttcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                        <  6:238198/138‑5 (MQ=255)
                                                                             tctttcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCAGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                        <  4:375124/138‑5 (MQ=255)
                                                                                 tcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGGCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATaat                                                    <  8:421926/138‑3 (MQ=255)
                                                                                     gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                               >  5:220833/1‑139 (MQ=255)
                                                                                     gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                               >  7:335567/1‑139 (MQ=255)
                                                                                      ccgccaACATAATCAAGGACCTTCACGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGAGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAtt                                              <  5:370323/139‑1 (MQ=255)
                                                                                                      gCACCTTCCAGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGt                              <  6:404332/139‑1 (MQ=255)
                                                                                                            tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                        >  5:424959/1‑103 (MQ=255)
                                                                                                            tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                        <  6:424959/107‑5 (MQ=255)
                                                                                                            tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                         >  6:257434/1‑138 (MQ=255)
                                                                                                            tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                         >  7:231264/1‑138 (MQ=255)
                                                                                                                 tCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATc                                                                                                            <  6:29903/50‑1 (MQ=255)
                                                                                                                 tCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATc                                                                                                            >  5:29903/1‑50 (MQ=255)
                                                                                                                        tCATGGCCATCTCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCAATTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt             <  2:199537/138‑1 (MQ=255)
                                                                                                                                  cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCCACATAATCAGATTCAGTCCGGAATGTCGTGACTTCCTAGGTTGCAGCCTCCCAGAGc  >  2:136344/1‑139 (MQ=255)
                                                                                                                                  cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt             >  6:326451/1‑128 (MQ=255)
                                                                                                                                  cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt             <  5:326451/128‑1 (MQ=255)
                                                                                                                                  cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCagag   >  3:161252/1‑138 (MQ=255)
                                                                                                                                  cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGACGTGACTTCATAGGTATCCTCCACCCagatc  >  2:440436/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CTCTCAACATACGAAGTGCATCCTCTTGAAATCTGTCCTCAGGCTTTCCGACGGTTCGAATCAGTTTCTGATCAATATCTTTCTTGCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGC  >  CP006881/2174445‑2174713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑