Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,622	T→A	T102S (ACA→TCA)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,622	0	T	A	100.0%	96.4 / NA	32	T102S (ACA→TCA)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/15);  total (17/15)

TTCCGACGGTTCGAATCAGTTTCTGATCAATATCTTTCTTGCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCT  >  CP006881/2174490‑2174756                                                                                                                                     |                                                                                                                                       ttCCAACGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATaaa                                                                                                                                  >  3:161212/1‑139 (MQ=255)   ccaaCGGTTCGAATCACTTTCTGATCAATATCATTCTTGCAGCCAAAATAATCAAGCACCTTCCCCTCCGCTCTCAGTGCCATCCAAGTGATCGTTAAATCTATGCGTTTTAGATCCTCTTCTAATGATTAGATATAtt                                                                                                                                >  6:370323/4‑139 (MQ=255)      aCGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCa                                                                                                                             <  1:277787/139‑1 (MQ=255)      aCGGTTCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCa                                                                                                                             <  8:108134/139‑1 (MQ=255)          ttCGAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGATTTATATACTCTTCTAATTATGAGATAAATTGAACTTc                                                                                                                         >  1:173784/1‑139 (MQ=255)             gAATCACTTTCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGa                                                                                                                      <  4:161212/139‑1 (MQ=255)                    tttCTGATCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTTCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGtctt                                                                                                               >  3:257117/1‑139 (MQ=255)                          aTCAATATCTTTCTTGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGtcttctgaaa                                                                                                         <  3:75016/139‑5 (MQ=255)                                 tctttcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                                                                   <  6:238198/138‑5 (MQ=255)                                 tctttcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCAGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                                                                   <  4:375124/138‑5 (MQ=255)                                     tcttGCCGCCAAAATAATCAAGCACCTTCCCGTCCGCTGGCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATaat                                                                                               <  8:421926/138‑3 (MQ=255)                                         gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                                                                          >  7:335567/1‑139 (MQ=255)                                         gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                                                                          >  5:220833/1‑139 (MQ=255)                                          ccgccaACATAATCAAGGACCTTCACGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGAGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAtt                                                                                         <  5:370323/139‑1 (MQ=255)                                                          gCACCTTCCAGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGt                                                                         <  6:404332/139‑1 (MQ=255)                                                                tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                                                                   <  6:424959/107‑5 (MQ=255)                                                                tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATcaaca                                                                                                   >  5:424959/1‑103 (MQ=255)                                                                tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                                                                    >  7:231264/1‑138 (MQ=255)                                                                tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                                                                    >  6:257434/1‑138 (MQ=255)                                                                            tCATGGCCATCTCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCAATTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                        <  2:199537/138‑1 (MQ=255)                                                                                      cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCCACATAATCAGATTCAGTCCGGAATGTCGTGACTTCCTAGGTTGCAGCCTCCCAGAGc                                             >  2:136344/1‑139 (MQ=255)                                                                                      cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                        >  6:326451/1‑128 (MQ=255)                                                                                      cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                        <  5:326451/128‑1 (MQ=255)                                                                                      cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCagag                                              >  3:161252/1‑138 (MQ=255)                                                                                      cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGACGTGACTTCATAGGTATCCTCCACCCagatc                                             >  2:440436/1‑137 (MQ=255)                                                                                              tCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACaataa                                      <  5:70793/138‑1 (MQ=255)                                                                                                  taaaTCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGt                                                                         >  5:177750/2‑99 (MQ=255)                                                                                                            cGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACaataa                                      <  4:228628/124‑1 (MQ=255)                                                                                                                 tagaTCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                   <  4:425963/135‑1 (MQ=255)                                                                                                                         cttctAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCaa           >  7:426818/1‑138 (MQ=255)                                                                                                                         cttctAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCaa           >  3:394330/1‑138 (MQ=255)                                                                                                                                  atgAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCt  >  7:111068/1‑138 (MQ=255)                                                                                                                                     |                                                                                                                                       TTCCGACGGTTCGAATCAGTTTCTGATCAATATCTTTCTTGCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCT  >  CP006881/2174490‑2174756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑