Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,174,662 C→A E88D (GAG→GAT)  U712_10875 ← CCA‑adding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,174,6620CA100.0% 111.1 / NA 37E88D (GAG→GAT) U712_10875CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/18);  total (19/18)

GCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTC  >  CP006881/2174530‑2174794
                                                                                                                                    |                                                                                                                                    
gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                                                                                                                >  7:335567/1‑139 (MQ=255)
gccgccAAAATAATCAAGCACCTTCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAt                                                                                                                                >  5:220833/1‑139 (MQ=255)
 ccgccaACATAATCAAGGACCTTCACGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGAGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGAtt                                                                                                                               <  5:370323/139‑1 (MQ=255)
                 gCACCTTCCAGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGt                                                                                                               <  6:404332/139‑1 (MQ=255)
                       tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                                                                                                          >  7:231264/1‑138 (MQ=255)
                       tCCCGTCCGCTGTCATGGCCATCGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACtt                                                                                                          >  6:257434/1‑138 (MQ=255)
                                   tCATGGCCATCTCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCAATTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                                                              <  2:199537/138‑1 (MQ=255)
                                             cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCCACATAATCAGATTCAGTCCGGAATGTCGTGACTTCCTAGGTTGCAGCCTCCCAGAGc                                                                                   >  2:136344/1‑139 (MQ=255)
                                             cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                                                              <  5:326451/128‑1 (MQ=255)
                                             cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCAt                                                                                              >  6:326451/1‑128 (MQ=255)
                                             cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCagag                                                                                    >  3:161252/1‑138 (MQ=255)
                                             cGCATTGATCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGACGTGACTTCATAGGTATCCTCCACCCagatc                                                                                   >  2:440436/1‑137 (MQ=255)
                                                     tCGTTAAATCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACaataa                                                                            <  5:70793/138‑1 (MQ=255)
                                                         taaaTCTCTGCGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGt                                                                                                               >  5:177750/2‑99 (MQ=255)
                                                                   cGTTTTAGATCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACaataa                                                                            <  4:228628/124‑1 (MQ=255)
                                                                        tagaTCCTCTTCTAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         <  4:425963/135‑1 (MQ=255)
                                                                                cttctAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCaa                                                 >  3:394330/1‑138 (MQ=255)
                                                                                cttctAATGATGAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCaa                                                 >  7:426818/1‑138 (MQ=255)
                                                                                         atgAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCt                                        >  7:111068/1‑138 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         >  1:397861/3‑119 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         <  4:92129/117‑1 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         >  4:235279/3‑119 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         >  3:92129/3‑119 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         <  3:235279/117‑1 (MQ=255)
                                                                                           gagATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGcc                                                         <  2:397861/117‑1 (MQ=255)
                                                                                            agATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGaaa                                    <  4:257117/138‑1 (MQ=255)
                                                                                                     gCACTTCTGACGGTCTTCTGAAATCAACCTAATCAGATTCAGTCCGGACTGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                            <  2:74929/138‑1 (MQ=255)
                                                                                                     gCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                            <  8:47574/138‑1 (MQ=255)
                                                                                                     gCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                            <  8:144325/138‑1 (MQ=255)
                                                                                                        cttCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTAc                        >  6:436188/3‑139 (MQ=255)
                                                                                                           cTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCg                                          <  7:267159/118‑1 (MQ=255)
                                                                                                           cTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCg                                          >  8:267159/1‑118 (MQ=255)
                                                                                                             gACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGAt                   >  8:276479/1‑139 (MQ=255)
                                                                                                              aCGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTg                     <  6:2132/136‑1 (MQ=255)
                                                                                                              aCGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATc                  <  1:440436/139‑1 (MQ=255)
                                                                                                                   cttctGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTg             >  6:396305/1‑139 (MQ=255)
                                                                                                                              aacATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTc  <  5:312328/137‑1 (MQ=255)
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GCCGCCGAAATAATCAAGCACCTTCCCATCCGCTGTCATGGCCATCGCATTAATCGTCAAATCTCTGCGTTTTAAATCCTCTTCTAATGATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTC  >  CP006881/2174530‑2174794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑