Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,749	C→A	T59T (ACG→ACT)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,749	0	C	A	100.0%	169.8 / NA	54	T59T (ACG→ACT)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/27);  total (27/27)

ATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGAT  >  CP006881/2174619‑2174880                                                                                                                                   |                                                                                                                                    atgAGATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCt                                                                                                                              >  7:111068/1‑138 (MQ=255)    agATAAATTGCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGaaa                                                                                                                          <  4:257117/138‑1 (MQ=255)             gCACTTCTGACGGTCTTCTGAAATCAACCTAATCAGATTCAGTCCGGACTGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                                                                                                                  <  2:74929/138‑1 (MQ=255)             gCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                                                                                                                  <  8:47574/138‑1 (MQ=255)             gCACTTCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                                                                                                                  <  8:144325/138‑1 (MQ=255)                cttCTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTAc                                                                                                              >  6:436188/3‑139 (MQ=255)                   cTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCg                                                                                                                                >  8:267159/1‑118 (MQ=255)                   cTGACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCg                                                                                                                                <  7:267159/118‑1 (MQ=255)                     gACGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGAt                                                                                                         >  8:276479/1‑139 (MQ=255)                      aCGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTg                                                                                                           <  6:2132/136‑1 (MQ=255)                      aCGGTCTTCTGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATc                                                                                                        <  1:440436/139‑1 (MQ=255)                           cttctGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTg                                                                                                   >  6:396305/1‑139 (MQ=255)                                      aacATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTc                                                                                        <  5:312328/137‑1 (MQ=255)                                               agATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCg                                                                               <  5:396305/138‑1 (MQ=255)                                                 aTTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGAc                                                                             <  6:220833/139‑1 (MQ=255)                                                  ttCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGAc                                                                             >  3:418645/1‑138 (MQ=255)                                                       tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATTTCGACATCTcc                                                                       >  5:362438/2‑139 (MQ=255)                                                       tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                       >  6:233714/2‑139 (MQ=255)                                                       tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                       >  1:245104/2‑139 (MQ=255)                                                       tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                       >  1:227693/2‑139 (MQ=255)                                                          ggAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAt                                                                    <  4:161252/139‑1 (MQ=255)                                                             aTGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgt                                                                 <  1:376235/139‑1 (MQ=255)                                                                tcgTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCTCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgttcg                                                              <  7:277219/137‑1 (MQ=255)                                                                                ttCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCa                                              >  6:202481/1‑139 (MQ=255)                                                                                 tCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCAc                                             >  6:175311/1‑139 (MQ=255)                                                                                         ccAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTg                                       <  5:233714/137‑1 (MQ=255)                                                                                             agCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcccc                                 >  5:175859/1‑137 (MQ=255)                                                                                             agCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcccc                                 >  4:437969/1‑137 (MQ=255)                                                                                               cacaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCg                               >  7:5317/1‑139 (MQ=255)                                                                                               cacaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCg                               >  2:278146/1‑139 (MQ=255)                                                                                                acaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGa                              <  8:111068/139‑1 (MQ=255)                                                                                                      atggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAAt                        <  2:245104/136‑1 (MQ=255)                                                                                                      atggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAAt                        <  2:227693/136‑1 (MQ=255)                                                                                                       tggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAg                                                                                                                       <  1:46247/41‑1 (MQ=255)                                                                                                       tggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAg                                                                                                                       >  2:46247/3‑43 (MQ=255)                                                                                                         gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGCTCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAg                     >  7:317090/1‑139 (MQ=255)                                                                                                         gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAg                                                    >  4:397264/1‑108 (MQ=255)                                                                                                         gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAg                                                    <  3:397264/108‑1 (MQ=255)                                                                                                         gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGCCAAAATAAg                     >  6:50796/1‑139 (MQ=255)                                                                                                         gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATaa                      <  5:202481/138‑1 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                                                                                                                  <  5:409466/43‑1 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCgtt                                                                                                                  >  6:409466/1‑43 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTcgcg                                                                                     >  7:138158/1‑72 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTcgcg                                                                                     <  8:138158/72‑1 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                   <  7:435454/122‑1 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                   >  8:435454/1‑122 (MQ=255)                                                                                                            ccGTGCTCTTTGCCTACATCAACAGTCAGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGGCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGCCAAAATAAGCCt                  >  7:245739/1‑139 (MQ=255)                                                                                                              gTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGa                >  4:196631/1‑139 (MQ=255)                                                                                                               tGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                   >  5:232398/1‑119 (MQ=255)                                                                                                               tGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                   <  6:232398/119‑1 (MQ=255)                                                                                                                       gCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGc        <  1:278146/138‑1 (MQ=255)                                                                                                                        ccTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCtt      <  4:418645/139‑1 (MQ=255)                                                                                                                            cATCAACAGTCCGCTGAAACATCCGATCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGCTCGTTCGCTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGa   <  4:119210/138‑1 (MQ=255)                                                                                                                            cATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGAt  >  3:214933/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                    ATGTGATAAATTGCACGTCTGACGGTCTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGAT  >  CP006881/2174619‑2174880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑