Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,144,389	A→G	S16P (TCT→CCT)	U712_05640 ←	UPF0234 protein yitk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,144,389	0	A	G	95.8%	218.4 / NA	73	S16P (TCT→CCT)	U712_05640	UPF0234 protein yitk
Reads supporting (aligned to +/- strand):  ref base A (1/1);  major base G (33/35);  minor base T (0/3);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GACAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTGATGAT  >  CP006881/1144255‑1144524                                                                                                                                        |                                                                                                                                        gCTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTTATGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGGTTTGCACTTCA‑GGCAAt                                                                                                                                     >  5:282291/1‑139 (MQ=255) gCTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAAt                                                                                                                                     >  8:27337/1‑139 (MQ=255) gCTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGAACTTCA‑GGCAAt                                                                                                                                     >  7:174010/1‑139 (MQ=255)  cTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAAtt                                                                                                                                    <  8:70879/139‑1 (MQ=255)       tttGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCAcc                                                                                                                               <  5:298175/139‑1 (MQ=255)       tttGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCAcc                                                                                                                               <  3:216326/139‑1 (MQ=255)           aaTTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTg                                                                                                                            <  4:173098/138‑1 (MQ=255)            aTTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGGACTGATTTCCTTCAGGGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCCCCTTGGa                                                                                                                          >  6:246174/1‑139 (MQ=255)                ctCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATCGCGCTTTTGCTGCCTTTAAACTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATAc                                                                                                                       <  2:443481/137‑1 (MQ=255)                     cGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTTGATACAatat                                                                                                                  >  5:22387/1‑138 (MQ=255)                          aCGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAat                                                                                                                    >  6:124971/1‑131 (MQ=255)                          aCGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAat                                                                                                                    <  5:124971/131‑1 (MQ=255)                            gAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGaa                                                                                                          <  1:286913/139‑1 (MQ=255)                                        ctctTTATCTAAAGACATGTCGGTTTAGCTGCCTTTACATTCATATCGGGTACTGATTTACTTCAGTGCGATTTGGATTCCGTTTTGCCCTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTATTTTGcc                                                                                              <  8:435110/139‑1 (MQ=255)                                        ctctTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GTCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGcc                                                                                              <  5:18099/139‑1 (MQ=255)                                        ctctTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGcc                                                                                              <  2:432131/139‑1 (MQ=255)                                         tctTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCa                                                                                             >  5:158449/1‑139 (MQ=255)                                          ctTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGcc                                                                                              >  8:309471/1‑137 (MQ=255)                                          ctTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGcc                                                                                              <  7:309471/137‑1 (MQ=255)                                             tATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACATTGGATACAATATCAAATGAACTTTCTTTTGCCatat                                                                                          >  5:425748/1‑138 (MQ=255)                                               tCTAAAGAAATGTCGCTTTTTCGTCCGTTAAAGTCATATCGGGTACTGCTTTAATTCATTGGGATTTTGCTTTCGTTTTGCACAGCATGAC‑ATTCCCCCTTGGATACAATATCAAAAGAACTGTCATTTGCGATATGa                                                                                        >  4:250375/1‑138 (MQ=255)                                                cTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGaa                                                                                       >  3:262937/1‑138 (MQ=255)                                                    aGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCt                                                                                  >  5:184655/1‑139 (MQ=255)                                                        aTGTCGCTTTTGCTGCCTTTACAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  7:381635/139‑1 (MQ=255)                                                        aTGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  1:70312/139‑1 (MQ=255)                                                        aTGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  6:371492/139‑1 (MQ=255)                                                        aTGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  1:63664/139‑1 (MQ=255)                                                        aTGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GACAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  2:29241/139‑1 (MQ=255)                                                         tGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATTTGAAAACCTCCAt                                                                              >  8:117442/1‑138 (MQ=255)                                                         tGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              >  4:391722/1‑138 (MQ=255)                                                                tttGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACAttt                                                                       >  4:448616/1‑138 (MQ=255)                                                                  tgctgcCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTaa                                                                     <  4:262937/138‑1 (MQ=255)                                                                         tttAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTAtgtg                                                             <  6:425748/139‑1 (MQ=255)                                                                           tAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTAtgtg                                                             <  4:142273/137‑1 (MQ=255)                                                                           tAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTAtgtg                                                             <  3:391722/137‑1 (MQ=255)                                                                           tAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTAtgtg                                                             <  1:334398/137‑1 (MQ=255)                                                                           tAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTAtgtg                                                             <  3:448616/137‑1 (MQ=255)                                                                                taatatCTTGTACTGATTTCCTTCAGTGAGATTTTGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATta                                                      <  3:250375/137‑1 (MQ=255)                                                                                tCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATta                                                      <  8:321507/139‑1 (MQ=255)                                                                                 cATATCGGTTACTGATTTGCTTGAGTGCGATTTGGATTGCGTTTTTCACTTCA‑GTCTATTGCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATAAGAAAACCTCCATATCATTTAACTTATGTGTTGATta                                                      <  5:402156/138‑1 (MQ=255)                                                                                  atatCGTGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGtt                                                           <  5:235183/132‑1 (MQ=255)                                                                                  atatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATTAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGtt                                                           >  6:235183/1‑132 (MQ=255)                                                                                  atatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGtt                                                           <  1:106105/132‑1 (MQ=255)                                                                                  atatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGtt                                                           >  2:106105/1‑132 (MQ=255)                                                                                   tatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCAcc                                                                                                                               <  6:18287/63‑1 (MQ=255)                                                                                   tatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCAcc                                                                                                                               >  5:18287/1‑63 (MQ=255)                                                                                   tatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCtttt                                                                                                 <  4:423969/93‑1 (MQ=255)                                                                                   tatCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCtttt                                                                                                 >  3:423969/1‑93 (MQ=255)                                                                                        gttACTGATTTCCTTCAGTGCGATTTGTATTGCGTTTTGCACTTCA‑TGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGaa                                              <  6:282291/137‑1 (MQ=255)                                                                                        ggTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATtata                                                    >  8:102172/1‑133 (MQ=255)                                                                                        ggTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATtata                                                    <  7:102172/133‑1 (MQ=255)                                                                                          tACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACAttt                                                                       <  8:309974/112‑1 (MQ=255)                                                                                          tACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACAttt                                                                       >  7:309974/1‑112 (MQ=255)                                                                                          tACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATta                                                      <  5:242696/129‑1 (MQ=255)                                                                                          tACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATta                                                      >  6:242696/1‑129 (MQ=255)                                                                                            cTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATa                                            >  4:281782/1‑137 (MQ=255)                                                                                                tttACTTAAGTGAGATTTTGATTGCTTTTTGCACTTAA‑GTCAATTAAACCTTTTATAAAATATAAAATTAAATTTCTTTTGCAATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATaaatat                                       <  6:22387/138‑4 (MQ=255)                                                                                                  tCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTc                                                                                                                                   >  5:222061/1‑44 (MQ=255)                                                                                                  tCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTc                                                                                                                                   <  6:222061/44‑1 (MQ=255)                                                                                                      tCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTACCTTATGTGTTGATTATAGTAGAATTAAGAGAAACTTc                                >  7:419943/1‑139 (MQ=255)                                                                                                         gTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACAttt                                                                       >  2:24612/1‑97 (MQ=255)                                                                                                         gTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACAttt                                                                       <  1:24612/97‑1 (MQ=255)                                                                                                         gTGCGATTTGGATTGCGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCaa                             >  4:291998/1‑139 (MQ=255)                                                                                                                 tGGATTGCGGTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTACATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACa                      <  6:250370/138‑1 (MQ=255)                                                                                                                   gATTGCGTGTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACaaa                    <  8:174010/138‑1 (MQ=255)                                                                                                                        cGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              <  2:269710/75‑1 (MQ=255)                                                                                                                        cGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCAt                                                                              >  1:269710/1‑75 (MQ=255)                                                                                                                        cGTTTTGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATc               >  8:436525/1‑138 (MQ=255)                                                                                                                             tGCACTTCA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTtg         >  4:206665/1‑139 (MQ=255)                                                                                                                                    cA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAACCCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTgatgat  >  7:363197/1‑139 (MQ=255)                                                                                                                                    cA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTgatgat  >  1:323172/1‑139 (MQ=255)                                                                                                                                    cA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTgatgat  >  3:283755/1‑139 (MQ=255)                                                                                                                                    cA‑GGCAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTgatgat  >  1:150672/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GCTTAATTTGAATTCGTCATCGGAAACGAGGACCAGCTCCTCTTTATCTAAAGAAATGTCGCTTTTGCTGCCTTTAAAGTCATATCGGGTACTGATTTCCTTCAGTGCGATTTGGATTGCGTTTTGCACTTCA‑GACAATTCCACCTTGGATACAATATCAAATGAACTTTCTTTTGCCATATGAAAACCTCCATAACATTTAACTTATGTGTTGATTATAGTAGAATAAAGAGAAACTTCCAACAGAACAAAGAATCAATTTGTGATGAT  >  CP006881/1144255‑1144524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑