Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,779	C→A	P49P (CCG→CCT)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,779	0	C	A	100.0%	155.5 / NA	50	P49P (CCG→CCT)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/23);  total (27/23)

CTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCT  >  CP006881/2174645‑2174905                                                                                                                                       |                                                                                                                               cttctGAAATCAACATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTg                                                                                                                            >  6:396305/1‑139 (MQ=255)            aacATAATCAGATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTc                                                                                                                 <  5:312328/137‑1 (MQ=255)                     agATTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCg                                                                                                        <  5:396305/138‑1 (MQ=255)                       aTTCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGAc                                                                                                      <  6:220833/139‑1 (MQ=255)                        ttCAGTCCGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGAc                                                                                                      >  3:418645/1‑138 (MQ=255)                             tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATTTCGACATCTcc                                                                                                >  5:362438/2‑139 (MQ=255)                             tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                                                >  1:227693/2‑139 (MQ=255)                             tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                                                >  6:233714/2‑139 (MQ=255)                             tccGGAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTcc                                                                                                >  1:245104/2‑139 (MQ=255)                                ggAATGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAt                                                                                             <  4:161252/139‑1 (MQ=255)                                   aTGTCGTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgt                                                                                          <  1:376235/139‑1 (MQ=255)                                      tcgTGACTTCATAGGTTTCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCTCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgttcg                                                                                       <  7:277219/137‑1 (MQ=255)                                                      ttCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCa                                                                       >  6:202481/1‑139 (MQ=255)                                                       tCATCCTCCCAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCAc                                                                      >  6:175311/1‑139 (MQ=255)                                                               ccAGAGCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTg                                                                <  5:233714/137‑1 (MQ=255)                                                                   agCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcccc                                                          >  5:175859/1‑137 (MQ=255)                                                                   agCACAATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcccc                                                          >  4:437969/1‑137 (MQ=255)                                                                     cacaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCg                                                        >  2:278146/1‑139 (MQ=255)                                                                     cacaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCg                                                        >  7:5317/1‑139 (MQ=255)                                                                      acaATAATGGTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGa                                                       <  8:111068/139‑1 (MQ=255)                                                                            atggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAAt                                                 <  2:245104/136‑1 (MQ=255)                                                                            atggTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAAt                                                 <  2:227693/136‑1 (MQ=255)                                                                               gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGCTCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAg                                              >  7:317090/1‑139 (MQ=255)                                                                               gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAg                                                                             >  4:397264/1‑108 (MQ=255)                                                                               gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAg                                                                             <  3:397264/108‑1 (MQ=255)                                                                               gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGCCAAAATAAg                                              >  6:50796/1‑139 (MQ=255)                                                                               gTCCCGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATaa                                               <  5:202481/138‑1 (MQ=255)                                                                                  ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTcgcg                                                                                                              >  7:138158/1‑72 (MQ=255)                                                                                  ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTcgcg                                                                                                              <  8:138158/72‑1 (MQ=255)                                                                                  ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                                            <  7:435454/122‑1 (MQ=255)                                                                                  ccGTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                                            >  8:435454/1‑122 (MQ=255)                                                                                  ccGTGCTCTTTGCCTACATCAACAGTCAGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGGCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGCCAAAATAAGCCt                                           >  7:245739/1‑139 (MQ=255)                                                                                    gTGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGa                                         >  4:196631/1‑139 (MQ=255)                                                                                     tGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                                            >  5:232398/1‑119 (MQ=255)                                                                                     tGCTCTTTGCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGcc                                                            <  6:232398/119‑1 (MQ=255)                                                                                             gCCTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGc                                 <  1:278146/138‑1 (MQ=255)                                                                                              ccTACATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCtt                               <  4:418645/139‑1 (MQ=255)                                                                                                  cATCAACAGTCCGCTGAAACATCCGATCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGCTCGTTCGCTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGa                            <  4:119210/138‑1 (MQ=255)                                                                                                  cATCAACAGTCCGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGAt                           >  3:214933/1‑139 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgttcg                                                                                       >  1:77246/1‑68 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGAtcgttcg                                                                                       <  2:77246/68‑1 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATcgttcgtt                                                                                     <  5:179206/70‑1 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATcgttcgtt                                                                                     >  6:179206/1‑70 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGc                                 <  2:284179/122‑1 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGc                                 >  1:284179/1‑122 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCgg                 <  5:175311/138‑1 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCg                  >  2:48304/1‑137 (MQ=255)                                                                                                             cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCg                  >  6:196152/1‑137 (MQ=255)                                                                                                                       gCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAg      >  1:295207/1‑139 (MQ=255)                                                                                                                           ttCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGATCGTTTCATATAGCTCTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCt  <  1:136344/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               CTTCTATAATCTTCATACTCTGATTCAGCCCGGAATGTAGTGACTTCATATGTTTCATCTTCCCAGAGCACAATAATCGTCCCGTGCTCTTTGCCTACATCAACCGTCCGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCT  >  CP006881/2174645‑2174905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑