BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,885	G→A	T14I (ACC→ATC)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,885	0	G	A	100.0%	128.6 / NA	41	T14I (ACC→ATC)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/20); total (21/20)

CGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATT  >  CP006881/2174753‑2175017

cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCgg                                                                                                                                 <  5:175311/138‑1 (MQ=255)

cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCg                                                                                                                                  >  2:48304/1‑137 (MQ=255)

cGCTGAAACAGCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCg                                                                                                                                  >  6:196152/1‑137 (MQ=255)

          gCCGTTCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAg                                                                                                                      >  1:295207/1‑139 (MQ=255)

              ttCTACTTGATCAGGTGCCGCATCTGTCGCGATATCGACATCTCCGATCGATCGTTTCATATAGCTCTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCt                                                                                                                  <  1:136344/139‑1 (MQ=255)

                        tcaggTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATaaaaa                                                                                                        <  6:175859/136‑1 (MQ=255)

                           ggTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACtt                                                                                                     >  3:341930/1‑139 (MQ=255)

                            gTGCCGCATCTGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACttt                                                                                                    >  8:84505/1‑138 (MQ=255)

                                       gTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCatgtgat                                                                                         <  6:362438/139‑6 (MQ=255)

                                        tCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCatgtgat                                                                                         <  3:437969/138‑6 (MQ=255)

                                                          cGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                      >  1:379007/1‑139 (MQ=255)

                                                               gttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                 <  7:276479/139‑1 (MQ=255)

                                                               ggtcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                 <  8:5317/137‑1 (MQ=255)

                                                                ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                      <  1:251312/133‑1 (MQ=255)

                                                                ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATAACTCCGGTTCTGCTAAAt                                                                      >  2:251312/1‑133 (MQ=255)

                                                                ttcgttTCATATAGCTGTCACAAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                 >  8:63956/1‑138 (MQ=255)

                                                                   gttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                             <  2:379007/139‑3 (MQ=255)

                                                                    tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                            <  4:341930/139‑1 (MQ=255)

                                                                    tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                            <  5:196152/139‑1 (MQ=255)

                                                                       cATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACTGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCt                                                          <  8:317090/138‑1 (MQ=255)

                                                                              ctgtCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATAc                                                  >  7:259509/4‑139 (MQ=255)

                                                                                 tCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                               >  3:45029/1‑139 (MQ=255)

                                                                                  cACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGc                                              >  7:364586/1‑139 (MQ=255)

                                                                                   aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACttt                                                                                                    <  8:385078/84‑2 (MQ=255)

                                                                                   aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACttt                                                                                                    >  7:385078/1‑83 (MQ=255)

                                                                                   aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCt                                             >  3:325403/1‑139 (MQ=255)

                                                                                   aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCATTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                               >  2:186532/1‑137 (MQ=255)

                                                                                           cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCt                                                                                                                  <  5:80999/62‑1 (MQ=255)

                                                                                           cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCt                                                                                                                  >  6:80999/1‑62 (MQ=255)

                                                                                           cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                      <  3:438339/106‑1 (MQ=255)

                                                                                           cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                      >  4:438339/1‑106 (MQ=255)

                                                                                           cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAttt                                     >  4:17661/1‑139 (MQ=255)

                                                                                                ccGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAtttttt                                  >  3:50622/1‑137 (MQ=255)

                                                                                                       aaTAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAgaagaa                          >  3:198228/1‑138 (MQ=255)

                                                                                                            gCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCa                    >  4:24222/1‑139 (MQ=255)

                                                                                                                 aTGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAtttt                >  3:361360/1‑138 (MQ=255)

                                                                                                                    gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag            <  3:17661/139‑5 (MQ=255)

                                                                                                                    gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCAAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag            <  3:196631/139‑5 (MQ=255)

                                                                                                                     cccGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaagc           <  7:84505/139‑6 (MQ=255)

                                                                                                                         cTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCAtct       <  8:259509/139‑1 (MQ=255)

                                                                                                                              aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt  <  4:45029/139‑1 (MQ=255)

CGCTGAAACAGCCTTTCTACCTGATCCGGTGCCGCATCAGTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATT  >  CP006881/2174753‑2175017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`