Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,919	G→T	Q3K (CAA→AAA)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,919	0	G	T	100.0%	159.5 / NA	51	Q3K (CAA→AAA)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/26);  total (25/26)

GTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATC  >  CP006881/2174792‑2175052                                                                                                                                |                                                                                                                                      gTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCatgtgat                                                                                                                            <  6:362438/139‑6 (MQ=255)  tCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCatgtgat                                                                                                                            <  3:437969/138‑6 (MQ=255)                    cGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                         >  1:379007/1‑139 (MQ=255)                         gttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                    <  7:276479/139‑1 (MQ=255)                         ggtcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                    <  8:5317/137‑1 (MQ=255)                          ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                         <  1:251312/133‑1 (MQ=255)                          ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATAACTCCGGTTCTGCTAAAt                                                                                                         >  2:251312/1‑133 (MQ=255)                          ttcgttTCATATAGCTGTCACAAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                    >  8:63956/1‑138 (MQ=255)                             gttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                <  2:379007/139‑3 (MQ=255)                              tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                                                               <  5:196152/139‑1 (MQ=255)                              tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                                                               <  4:341930/139‑1 (MQ=255)                                 cATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACTGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCt                                                                                             <  8:317090/138‑1 (MQ=255)                                        ctgtCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATAc                                                                                     >  7:259509/4‑139 (MQ=255)                                           tCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                                                                  >  3:45029/1‑139 (MQ=255)                                            cACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGc                                                                                 >  7:364586/1‑139 (MQ=255)                                             aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCt                                                                                >  3:325403/1‑139 (MQ=255)                                             aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCATTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                                                                  >  2:186532/1‑137 (MQ=255)                                                     cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                         <  3:438339/106‑1 (MQ=255)                                                     cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                         >  4:438339/1‑106 (MQ=255)                                                     cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAttt                                                                        >  4:17661/1‑139 (MQ=255)                                                          ccGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAtttttt                                                                     >  3:50622/1‑137 (MQ=255)                                                                 aaTAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAgaagaa                                                             >  3:198228/1‑138 (MQ=255)                                                                      gCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCa                                                       >  4:24222/1‑139 (MQ=255)                                                                           aTGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAtttt                                                   >  3:361360/1‑138 (MQ=255)                                                                              gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                               <  3:17661/139‑5 (MQ=255)                                                                              gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCAAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                               <  3:196631/139‑5 (MQ=255)                                                                               cccGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaagc                                              <  7:84505/139‑6 (MQ=255)                                                                                   cTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCAtct                                          <  8:259509/139‑1 (MQ=255)                                                                                        aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt                                     <  4:45029/139‑1 (MQ=255)                                                                                             gatGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGc                                <  4:325403/137‑1 (MQ=255)                                                                                                 cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                <  4:106335/71‑3 (MQ=255)                                                                                                 cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                >  3:106335/1‑69 (MQ=255)                                                                                                 cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCcttt                            >  6:81322/1‑137 (MQ=255)                                                                                                  ggAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTg                           >  7:206762/1‑139 (MQ=255)                                                                                                      cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                        >  5:198891/1‑138 (MQ=255)                                                                                                      cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                        >  4:168207/1‑138 (MQ=255)                                                                                                      cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTACTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                        >  5:355103/1‑138 (MQ=255)                                                                                                          ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                   <  5:81322/139‑2 (MQ=255)                                                                                                          ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                   <  1:48304/139‑2 (MQ=255)                                                                                                              gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc               >  5:183583/1‑139 (MQ=255)                                                                                                              gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc               >  4:165853/1‑139 (MQ=255)                                                                                                              gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc               >  8:373943/1‑139 (MQ=255)                                                                                                               tGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct              <  1:186532/139‑1 (MQ=255)                                                                                                                   ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct              >  6:436952/1‑135 (MQ=255)                                                                                                                   ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct              <  5:436952/135‑1 (MQ=255)                                                                                                                    tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTTCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg           <  6:355103/137‑1 (MQ=255)                                                                                                                    tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg           <  6:198891/137‑1 (MQ=255)                                                                                                                    tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCt         >  6:217022/1‑139 (MQ=255)                                                                                                                        aaaaaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTc     <  3:24222/139‑1 (MQ=255)                                                                                                                           aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc  <  5:217022/139‑1 (MQ=255)                                                                                                                           aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc  <  8:364586/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GTCGCGATATCGACATCTCCGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATC  >  CP006881/2174792‑2175052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑