Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,928	T→G	intergenic (‑3/+2)	U712_10875 ← / ← U712_10880	CCA‑adding enzyme/putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,928	0	T	G	100.0%	168.8 / NA	52	intergenic (‑3/+2)	U712_10875/U712_10880	CCA‑adding enzyme/putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/24);  total (28/24)

CGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCT  >  CP006881/2174811‑2175056                                                                                                                      |                                                                                                                                 cGATCGTTCGTTTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                             >  1:379007/1‑139 (MQ=255)      gttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                        <  7:276479/139‑1 (MQ=255)      ggtcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                        <  8:5317/137‑1 (MQ=255)       ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                             <  1:251312/133‑1 (MQ=255)       ttcgttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATAACTCCGGTTCTGCTAAAt                                                                                                             >  2:251312/1‑133 (MQ=255)       ttcgttTCATATAGCTGTCACAAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCa                                                                                                        >  8:63956/1‑138 (MQ=255)          gttTCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                    <  2:379007/139‑3 (MQ=255)           tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                                                                   <  5:196152/139‑1 (MQ=255)           tttCATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAAt                                                                                                   <  4:341930/139‑1 (MQ=255)              cATATAGCTGTCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACTGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCt                                                                                                 <  8:317090/138‑1 (MQ=255)                     ctgtCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATAc                                                                                         >  7:259509/4‑139 (MQ=255)                        tCACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                                                                      >  3:45029/1‑139 (MQ=255)                         cACGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGc                                                                                     >  7:364586/1‑139 (MQ=255)                          aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCt                                                                                    >  3:325403/1‑139 (MQ=255)                          aCGAACTGCGCCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCATTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTgg                                                                                      >  2:186532/1‑137 (MQ=255)                                  cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                             <  3:438339/106‑1 (MQ=255)                                  cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAAt                                                                                                             >  4:438339/1‑106 (MQ=255)                                  cgcCCCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAttt                                                                            >  4:17661/1‑139 (MQ=255)                                       ccGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAAtttttt                                                                         >  3:50622/1‑137 (MQ=255)                                              aaTAAGCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAgaagaa                                                                 >  3:198228/1‑138 (MQ=255)                                                   gCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCa                                                           >  4:24222/1‑139 (MQ=255)                                                        aTGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAtttt                                                       >  3:361360/1‑138 (MQ=255)                                                           gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                                   <  3:17661/139‑5 (MQ=255)                                                           gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCAAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                                   <  3:196631/139‑5 (MQ=255)                                                            cccGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaagc                                                  <  7:84505/139‑6 (MQ=255)                                                                cTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCAtct                                              <  8:259509/139‑1 (MQ=255)                                                                     aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt                                         <  4:45029/139‑1 (MQ=255)                                                                          gatGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGc                                    <  4:325403/137‑1 (MQ=255)                                                                              cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                    >  3:106335/1‑69 (MQ=255)                                                                              cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATaaa                                                                                                    <  4:106335/71‑3 (MQ=255)                                                                              cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCcttt                                >  6:81322/1‑137 (MQ=255)                                                                               ggAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTg                               >  7:206762/1‑139 (MQ=255)                                                                                   cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                            >  5:198891/1‑138 (MQ=255)                                                                                   cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                            >  4:168207/1‑138 (MQ=255)                                                                                   cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTACTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                            >  5:355103/1‑138 (MQ=255)                                                                                       ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                       <  5:81322/139‑2 (MQ=255)                                                                                       ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                       <  1:48304/139‑2 (MQ=255)                                                                                           gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                   >  8:373943/1‑139 (MQ=255)                                                                                           gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                   >  4:165853/1‑139 (MQ=255)                                                                                           gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                   >  5:183583/1‑139 (MQ=255)                                                                                            tGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                  <  1:186532/139‑1 (MQ=255)                                                                                                ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                  >  6:436952/1‑135 (MQ=255)                                                                                                ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                  <  5:436952/135‑1 (MQ=255)                                                                                                 tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTTCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg               <  6:355103/137‑1 (MQ=255)                                                                                                 tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg               <  6:198891/137‑1 (MQ=255)                                                                                                 tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCt             >  6:217022/1‑139 (MQ=255)                                                                                                     aaaaaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTc         <  3:24222/139‑1 (MQ=255)                                                                                                        aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc      <  5:217022/139‑1 (MQ=255)                                                                                                        aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc      <  8:364586/139‑1 (MQ=255)                                                                                                             tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct  >  1:99172/2‑138 (MQ=255)                                                                                                             tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct  >  4:189394/2‑138 (MQ=255)                                                                                                             tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct  >  8:361179/2‑138 (MQ=255)                                                                                                                      |                                                                                                                                 CGATCGTTCGTTTCATATAGCTATCACGAACTGCGCCTCCGACAAAATAAGCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCT  >  CP006881/2174811‑2175056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑