Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,996	C→T	E356E (GAG→GAA)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,996	0	C	T	100.0%	180.4 / NA	58	E356E (GAG→GAA)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/24);  total (34/24)

GCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCT  >  CP006881/2174861‑2175118                                                                                                                                        |                                                                                                                           gCCTGATGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCa                                                                                                                         >  4:24222/1‑139 (MQ=255)      aTGGCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAtttt                                                                                                                     >  3:361360/1‑138 (MQ=255)         gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                                                                                                 <  3:17661/139‑5 (MQ=255)         gCCCGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCAAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaag                                                                                                                 <  3:196631/139‑5 (MQ=255)          cccGCTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCAttttcaagc                                                                                                                <  7:84505/139‑6 (MQ=255)              cTTCGATTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCAtct                                                                                                            <  8:259509/139‑1 (MQ=255)                   aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt                                                                                                       <  4:45029/139‑1 (MQ=255)                        gatGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGc                                                                                                  <  4:325403/137‑1 (MQ=255)                            cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCcttt                                                                                              >  6:81322/1‑137 (MQ=255)                             ggAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTg                                                                                             >  7:206762/1‑139 (MQ=255)                                 cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                          >  4:168207/1‑138 (MQ=255)                                 cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                          >  5:198891/1‑138 (MQ=255)                                 cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTACTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                          >  5:355103/1‑138 (MQ=255)                                     ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                     <  1:48304/139‑2 (MQ=255)                                     ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                     <  5:81322/139‑2 (MQ=255)                                         gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                 >  4:165853/1‑139 (MQ=255)                                         gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                 >  5:183583/1‑139 (MQ=255)                                         gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                 >  8:373943/1‑139 (MQ=255)                                          tGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                <  1:186532/139‑1 (MQ=255)                                              ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                >  6:436952/1‑135 (MQ=255)                                              ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                <  5:436952/135‑1 (MQ=255)                                               tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTTCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                             <  6:355103/137‑1 (MQ=255)                                               tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                             <  6:198891/137‑1 (MQ=255)                                               tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCt                                                                           >  6:217022/1‑139 (MQ=255)                                                   aaaaaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTc                                                                       <  3:24222/139‑1 (MQ=255)                                                      aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                    <  5:217022/139‑1 (MQ=255)                                                      aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                    <  8:364586/139‑1 (MQ=255)                                                           tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                >  4:189394/2‑138 (MQ=255)                                                           tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                >  1:99172/2‑138 (MQ=255)                                                           tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                >  8:361179/2‑138 (MQ=255)                                                                         cTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                 >  6:276055/1‑139 (MQ=255)                                                                             ggTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCggg                                             <  4:198228/139‑2 (MQ=255)                                                                                 cTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGt                                         >  2:386335/1‑139 (MQ=255)                                                                                    cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                       <  2:295207/138‑1 (MQ=255)                                                                                    cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                      >  2:151884/1‑139 (MQ=255)                                                                                    cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                      >  2:347496/1‑139 (MQ=255)                                                                                    cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                      >  5:138452/1‑139 (MQ=255)                                                                                    cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCGAAAATGCTCATAGCGCGGGCTGTTGc                                      >  8:380593/1‑139 (MQ=255)                                                                                         tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                 <  3:165853/139‑3 (MQ=255)                                                                                         tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                 <  7:373943/139‑3 (MQ=255)                                                                                              aTAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTGCATCTTCTAAACTGCTCATCGCGCGGGCTGTTGCGGCCGTAAca                            <  8:245739/139‑2 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                       >  3:2666/1‑119 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                       <  4:2666/119‑1 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                   >  5:143029/1‑139 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                   >  5:140729/1‑139 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                   >  3:350105/1‑139 (MQ=255)                                                                                                       ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATATTCTAAAATGCTAATCGCGCGGGCTGTTGCGGCCGTAACATCACCGAc                    >  8:198371/1‑138 (MQ=255)                                                                                                            aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                 >  1:227237/1‑104 (MQ=255)                                                                                                            aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                 <  2:227237/104‑1 (MQ=255)                                                                                                            aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccac              >  3:393111/1‑139 (MQ=255)                                                                                                               ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca            >  7:244255/1‑138 (MQ=255)                                                                                                               ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca            >  5:160639/1‑138 (MQ=255)                                                                                                               ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca            >  2:196375/1‑138 (MQ=255)                                                                                                                 cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa          >  6:354358/1‑138 (MQ=255)                                                                                                                 cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa          >  5:15404/1‑138 (MQ=255)                                                                                                                 cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGATCATCGCGCGGGCTGTTGCGGCCGTACCATCACCGACATCCCCCaaa          >  8:45242/1‑138 (MQ=255)                                                                                                                    aCAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATc      <  6:140729/139‑1 (MQ=255)                                                                                                                        ttttttTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCt  <  4:50622/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           GCCTGATGGCCCGCTTCGATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCT  >  CP006881/2174861‑2175118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑