Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,014	C→T	M350I (ATG→ATA)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,014	0	C	T	100.0%	184.4 / NA	59	M350I (ATG→ATA)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/27);  total (32/27)

ATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATA  >  CP006881/2174879‑2175146                                                                                                                                        |                                                                                                                                     aTTAAGATGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTAtt                                                                                                                                   <  4:45029/139‑1 (MQ=255)      gatGCGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGc                                                                                                                              <  4:325403/137‑1 (MQ=255)          cGGAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCcttt                                                                                                                          >  6:81322/1‑137 (MQ=255)           ggAGCACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTg                                                                                                                         >  7:206762/1‑139 (MQ=255)               cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                                      >  5:198891/1‑138 (MQ=255)               cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                                      >  4:168207/1‑138 (MQ=255)               cACGGGAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTACTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTaa                                                                                                                      >  5:355103/1‑138 (MQ=255)                   ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                                                 <  1:48304/139‑2 (MQ=255)                   ggAAGTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACga                                                                                                                 <  5:81322/139‑2 (MQ=255)                       gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                             >  8:373943/1‑139 (MQ=255)                       gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                             >  5:183583/1‑139 (MQ=255)                       gTGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgc                                                                                                             >  4:165853/1‑139 (MQ=255)                        tGCTTTGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                                            <  1:186532/139‑1 (MQ=255)                            ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                                            >  6:436952/1‑135 (MQ=255)                            ttGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACgattgct                                                                                                            <  5:436952/135‑1 (MQ=255)                             tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTTCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                                                         <  6:355103/137‑1 (MQ=255)                             tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAg                                                                                                         <  6:198891/137‑1 (MQ=255)                             tGATAAAAACTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCt                                                                                                       >  6:217022/1‑139 (MQ=255)                                 aaaaaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTc                                                                                                   <  3:24222/139‑1 (MQ=255)                                    aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                                                <  5:217022/139‑1 (MQ=255)                                    aaCTTTTTCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtc                                                                                                <  8:364586/139‑1 (MQ=255)                                         tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                                            >  1:99172/2‑138 (MQ=255)                                         tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                                            >  8:361179/2‑138 (MQ=255)                                         tttCCATGTGATCACTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCAtcttct                                                                                            >  4:189394/2‑138 (MQ=255)                                                       cTCCGGTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                             >  6:276055/1‑139 (MQ=255)                                                           ggTTCTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCggg                                                                         <  4:198228/139‑2 (MQ=255)                                                               cTGCTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGt                                                                     >  2:386335/1‑139 (MQ=255)                                                                  cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                                   <  2:295207/138‑1 (MQ=255)                                                                  cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                                  >  2:347496/1‑139 (MQ=255)                                                                  cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                                  >  5:138452/1‑139 (MQ=255)                                                                  cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGc                                                                  >  2:151884/1‑139 (MQ=255)                                                                  cTAAATCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCGAAAATGCTCATAGCGCGGGCTGTTGc                                                                  >  8:380593/1‑139 (MQ=255)                                                                       tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                                             <  3:165853/139‑3 (MQ=255)                                                                       tCGGCATAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGccg                                                             <  7:373943/139‑3 (MQ=255)                                                                            aTAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTGCATCTTCTAAACTGCTCATCGCGCGGGCTGTTGCGGCCGTAAca                                                        <  8:245739/139‑2 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                                   >  3:2666/1‑119 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTg                                                                   <  4:2666/119‑1 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                               >  3:350105/1‑139 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                               >  5:143029/1‑139 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                               >  5:140729/1‑139 (MQ=255)                                                                                     ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATATTCTAAAATGCTAATCGCGCGGGCTGTTGCGGCCGTAACATCACCGAc                                                >  8:198371/1‑138 (MQ=255)                                                                                          aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                             <  2:227237/104‑1 (MQ=255)                                                                                          aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATcgcg                                                                             >  1:227237/1‑104 (MQ=255)                                                                                          aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccac                                          >  3:393111/1‑139 (MQ=255)                                                                                             ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                        >  7:244255/1‑138 (MQ=255)                                                                                             ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                        >  2:196375/1‑138 (MQ=255)                                                                                             ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                        >  5:160639/1‑138 (MQ=255)                                                                                               cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                      >  5:15404/1‑138 (MQ=255)                                                                                               cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                      >  6:354358/1‑138 (MQ=255)                                                                                               cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGATCATCGCGCGGGCTGTTGCGGCCGTACCATCACCGACATCCCCCaaa                                      >  8:45242/1‑138 (MQ=255)                                                                                                  aCAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATc                                  <  6:140729/139‑1 (MQ=255)                                                                                                      ttttttTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCt                              <  4:50622/139‑1 (MQ=255)                                                                                                                    aattCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                <  4:393111/136‑3 (MQ=255)                                                                                                                     attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat               <  6:183583/137‑1 (MQ=255)                                                                                                                     attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat               <  7:361179/137‑1 (MQ=255)                                                                                                                     attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat               <  3:189394/137‑1 (MQ=255)                                                                                                                     attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat               <  1:386335/137‑1 (MQ=255)                                                                                                                     attCATTTTCAAGCATCTCTAATGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat               <  5:50796/137‑1 (MQ=255)                                                                                                                                  caTCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATa  <  5:354358/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     ATTAAGGTGCGGAGCACGGGAAGTGCTTTGATAAAAACTTGTTCCATATTATCACTCCGGTTCTGCTAAATCGGCATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATA  >  CP006881/2174879‑2175146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑