Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,086	T→C	A326T (GCA→ACG)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,086	0	T	C	100.0%	205.7 / NA	63	A326T (GCA→ACG)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

ATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAG  >  CP006881/2174954‑2175212                                                                                                                                     |                                                                                                                               aTAAATCTGTTCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTGCATCTTCTAAACTGCTCATCGCGCGGGCTGTTGCGGCCGTAAca                                                                                                                          <  8:245739/139‑2 (MQ=255)          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  5:140729/1‑139 (MQ=255)          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  5:143029/1‑139 (MQ=255)          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  3:350105/1‑139 (MQ=255)          ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATATTCTAAAATGCTAATCGCGCGGGCTGTTGCGGCCGTAACATCACCGAc                                                                                                                  >  8:198371/1‑138 (MQ=255)               aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccac                                                                                                            >  3:393111/1‑139 (MQ=255)                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  2:196375/1‑138 (MQ=255)                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  7:244255/1‑138 (MQ=255)                  ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  5:160639/1‑138 (MQ=255)                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                                                                                        >  5:15404/1‑138 (MQ=255)                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                                                                                        >  6:354358/1‑138 (MQ=255)                    cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGATCATCGCGCGGGCTGTTGCGGCCGTACCATCACCGACATCCCCCaaa                                                                                                        >  8:45242/1‑138 (MQ=255)                       aCAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATc                                                                                                    <  6:140729/139‑1 (MQ=255)                           ttttttTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCt                                                                                                <  4:50622/139‑1 (MQ=255)                                         aattCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  <  4:393111/136‑3 (MQ=255)                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  3:189394/137‑1 (MQ=255)                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  6:183583/137‑1 (MQ=255)                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  1:386335/137‑1 (MQ=255)                                          attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  7:361179/137‑1 (MQ=255)                                          attCATTTTCAAGCATCTCTAATGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  5:50796/137‑1 (MQ=255)                                                       caTCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATa                                                                    <  5:354358/138‑1 (MQ=255)                                                                  ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  8:206762/137‑1 (MQ=255)                                                                  ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  2:99172/137‑1 (MQ=255)                                                                  ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  4:379492/3‑139 (MQ=255)                                                                  ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTATTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  6:160639/137‑1 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 >  8:152493/1‑113 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  7:152493/113‑1 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                       >  3:162694/1‑139 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                       >  7:233360/1‑139 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  4:340310/1‑137 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  3:340310/137‑1 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  2:198047/1‑137 (MQ=255)                                                                    gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  1:198047/137‑1 (MQ=255)                                                                     ccTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttgt                                                      >  4:97309/1‑139 (MQ=255)                                                                       tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  6:441813/3‑78 (MQ=255)                                                                       tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 <  5:441813/76‑1 (MQ=255)                                                                       tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTcaca                                                                                            <  7:417919/97‑1 (MQ=255)                                                                       tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTcaca                                                                                            >  8:417919/3‑99 (MQ=255)                                                                       tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    >  6:309798/3‑139 (MQ=255)                                                                         tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCTCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  7:236872/137‑1 (MQ=255)                                                                         tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  5:309798/137‑1 (MQ=255)                                                                         tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  4:361360/137‑1 (MQ=255)                                                                         tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  3:97309/137‑1 (MQ=255)                                                                             aaaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATa                                               <  4:350105/138‑1 (MQ=255)                                                                              aaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACa                                             <  1:151884/139‑1 (MQ=255)                                                                                cgattgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAg                                           >  2:435073/4‑139 (MQ=255)                                                                                   ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  8:233360/139‑1 (MQ=255)                                                                                   ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  1:347496/139‑1 (MQ=255)                                                                                         aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  5:197354/133‑1 (MQ=255)                                                                                         aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        >  6:197354/1‑133 (MQ=255)                                                                                           cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  <  1:165326/89‑3 (MQ=255)                                                                                           cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  >  2:165326/1‑87 (MQ=255)                                                                                              ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  6:176735/1‑111 (MQ=255)                                                                                              ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  5:176735/111‑1 (MQ=255)                                                                                                gacagtctTAAATGCTCATCGGGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCTAAAATCCGCTCACATTGTTTTTTATAATCTCAGGTATACCGCCAATGTTTGTTCCAATACAAGTCACTCCGCAAGCCa                            <  7:6450/133‑1 (MQ=255)                                                                                                   cttctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGc                        >  8:242440/1‑139 (MQ=255)                                                                                                     tctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGctt                      >  2:399066/1‑137 (MQ=255)                                                                                                        aaaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaa                   >  2:365596/1‑139 (MQ=255)                                                                                                         aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                  <  7:380593/139‑1 (MQ=255)                                                                                                         aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                  <  7:198371/139‑1 (MQ=255)                                                                                                                cATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCAGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGATTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaagactgtca           >  8:6450/1‑133 (MQ=255)                                                                                                                 aTCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGAc            >  7:55437/1‑137 (MQ=255)                                                                                                                         ggcTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTGTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAg  <  8:302355/137‑1 (MQ=255)                                                                                                                                     |                                                                                                                               ATAGATCTGTTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAG  >  CP006881/2174954‑2175212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑