Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,095	G→A	S323G (AGC→GGT)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,095	0	G	A	100.0%	182.1 / NA	62	S323G (AGC→GGT)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/32);  total (30/32)

TTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAG  >  CP006881/2174963‑2175212                                                                                                                                     |                                                                                                                      ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  5:143029/1‑139 (MQ=255) ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  5:140729/1‑139 (MQ=255) ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  3:350105/1‑139 (MQ=255) ttCATACTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATATTCTAAAATGCTAATCGCGCGGGCTGTTGCGGCCGTAACATCACCGAc                                                                                                                  >  8:198371/1‑138 (MQ=255)      aCTGGCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccac                                                                                                            >  3:393111/1‑139 (MQ=255)         ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  7:244255/1‑138 (MQ=255)         ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  5:160639/1‑138 (MQ=255)         ggCTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATccacca                                                                                                          >  2:196375/1‑138 (MQ=255)           cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                                                                                        >  5:15404/1‑138 (MQ=255)           cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCaaa                                                                                                        >  6:354358/1‑138 (MQ=255)           cTGACAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGATCATCGCGCGGGCTGTTGCGGCCGTACCATCACCGACATCCCCCaaa                                                                                                        >  8:45242/1‑138 (MQ=255)              aCAATTTTTTTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATc                                                                                                    <  6:140729/139‑1 (MQ=255)                  ttttttTAGAAGAAAATTCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCt                                                                                                <  4:50622/139‑1 (MQ=255)                                aattCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  <  4:393111/136‑3 (MQ=255)                                 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  7:361179/137‑1 (MQ=255)                                 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  6:183583/137‑1 (MQ=255)                                 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  3:189394/137‑1 (MQ=255)                                 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  1:386335/137‑1 (MQ=255)                                 attCATTTTCAAGCATCTCTAATGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  5:50796/137‑1 (MQ=255)                                              caTCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATa                                                                    <  5:354358/138‑1 (MQ=255)                                                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  4:379492/3‑139 (MQ=255)                                                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  2:99172/137‑1 (MQ=255)                                                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  8:206762/137‑1 (MQ=255)                                                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTATTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  6:160639/137‑1 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 >  8:152493/1‑113 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                 <  7:152493/113‑1 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                       >  7:233360/1‑139 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                       >  3:162694/1‑139 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  2:198047/1‑137 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  1:198047/137‑1 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  4:340310/1‑137 (MQ=255)                                                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  3:340310/137‑1 (MQ=255)                                                            ccTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttgt                                                      >  4:97309/1‑139 (MQ=255)                                                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 >  6:441813/3‑78 (MQ=255)                                                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACa                                                                                                                 <  5:441813/76‑1 (MQ=255)                                                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTcaca                                                                                            <  7:417919/97‑1 (MQ=255)                                                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTcaca                                                                                            >  8:417919/3‑99 (MQ=255)                                                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    >  6:309798/3‑139 (MQ=255)                                                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCTCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  7:236872/137‑1 (MQ=255)                                                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  5:309798/137‑1 (MQ=255)                                                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  4:361360/137‑1 (MQ=255)                                                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                    <  3:97309/137‑1 (MQ=255)                                                                    aaaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATa                                               <  4:350105/138‑1 (MQ=255)                                                                     aaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACa                                             <  1:151884/139‑1 (MQ=255)                                                                       cgattgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAg                                           >  2:435073/4‑139 (MQ=255)                                                                          ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  8:233360/139‑1 (MQ=255)                                                                          ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  1:347496/139‑1 (MQ=255)                                                                                aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        <  5:197354/133‑1 (MQ=255)                                                                                aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                        >  6:197354/1‑133 (MQ=255)                                                                                  cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  <  1:165326/89‑3 (MQ=255)                                                                                  cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                  >  2:165326/1‑87 (MQ=255)                                                                                     ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         >  6:176735/1‑111 (MQ=255)                                                                                     ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                         <  5:176735/111‑1 (MQ=255)                                                                                       gacagtctTAAATGCTCATCGGGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCTAAAATCCGCTCACATTGTTTTTTATAATCTCAGGTATACCGCCAATGTTTGTTCCAATACAAGTCACTCCGCAAGCCa                            <  7:6450/133‑1 (MQ=255)                                                                                          cttctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGc                        >  8:242440/1‑139 (MQ=255)                                                                                            tctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGctt                      >  2:399066/1‑137 (MQ=255)                                                                                               aaaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaa                   >  2:365596/1‑139 (MQ=255)                                                                                                aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                  <  7:380593/139‑1 (MQ=255)                                                                                                aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                  <  7:198371/139‑1 (MQ=255)                                                                                                       cATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCAGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGATTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaagactgtca           >  8:6450/1‑133 (MQ=255)                                                                                                        aTCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGAc            >  7:55437/1‑137 (MQ=255)                                                                                                                ggcTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTGTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAg  <  8:302355/137‑1 (MQ=255)                                                                                                                                     |                                                                                                                      TTCATACTGGCTGACAATTTTTTGTGAAGAAAACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAG  >  CP006881/2174963‑2175212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑