Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,128 C→T K312K (AAG→AAA)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,1280CT100.0% 168.7 / NA 54K312K (AAG→AAA) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/36);  total (18/36)

AACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAG  >  CP006881/2174994‑2175259
                                                                                                                                      |                                                                                                                                   
aattCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                                                                 <  4:393111/136‑3 (MQ=255)
 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  3:189394/137‑1 (MQ=255)
 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  7:361179/137‑1 (MQ=255)
 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  1:386335/137‑1 (MQ=255)
 attCATTTTCAAGCATCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  6:183583/137‑1 (MQ=255)
 attCATTTTCAAGCATCTCTAATGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  5:50796/137‑1 (MQ=255)
              caTCTCTATTGCCGCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATa                                                                                                                   <  5:354358/138‑1 (MQ=255)
                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  8:206762/137‑1 (MQ=255)
                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        >  4:379492/3‑139 (MQ=255)
                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  2:99172/137‑1 (MQ=255)
                         ccgCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTATTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  6:160639/137‑1 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                >  8:152493/1‑113 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCGTCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTttat                                                                                                                                <  7:152493/113‑1 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                                                                      >  7:233360/1‑139 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttg                                                                                                      >  3:162694/1‑139 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        >  4:340310/1‑137 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  3:340310/137‑1 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        >  2:198047/1‑137 (MQ=255)
                           gCCTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  1:198047/137‑1 (MQ=255)
                            ccTTTGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtttgt                                                                                                     >  4:97309/1‑139 (MQ=255)
                              tttGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                                                                   >  6:309798/3‑139 (MQ=255)
                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCTCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                                                                   <  7:236872/137‑1 (MQ=255)
                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                                                                   <  3:97309/137‑1 (MQ=255)
                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                                                                   <  4:361360/137‑1 (MQ=255)
                                tGTAAAACGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTc                                                                                                   <  5:309798/137‑1 (MQ=255)
                                    aaaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATa                                                                                              <  4:350105/138‑1 (MQ=255)
                                     aaCGATTGCTTAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACa                                                                                            <  1:151884/139‑1 (MQ=255)
                                       cgattgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAg                                                                                          >  2:435073/4‑139 (MQ=255)
                                          ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                                                                       <  8:233360/139‑1 (MQ=255)
                                          ttgcttAGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                                                                       <  1:347496/139‑1 (MQ=255)
                                                aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                                                                       >  6:197354/1‑133 (MQ=255)
                                                aGCTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCa                                                                                       <  5:197354/133‑1 (MQ=255)
                                                  cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                                                                 >  2:165326/1‑87 (MQ=255)
                                                  cTGTTCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTttttta                                                                                                                                 <  1:165326/89‑3 (MQ=255)
                                                     ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        <  5:176735/111‑1 (MQ=255)
                                                     ttCATCTTCTAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAAtgtt                                                                                                        >  6:176735/1‑111 (MQ=255)
                                                       gacagtctTAAATGCTCATCGGGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCTAAAATCCGCTCACATTGTTTTTTATAATCTCAGGTATACCGCCAATGTTTGTTCCAATACAAGTCACTCCGCAAGCCa                                                                           <  7:6450/133‑1 (MQ=255)
                                                          cttctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGc                                                                       >  8:242440/1‑139 (MQ=255)
                                                            tctAAAATGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGctt                                                                     >  2:399066/1‑137 (MQ=255)
                                                               aaaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaa                                                                  >  2:365596/1‑139 (MQ=255)
                                                                aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                                                                 <  7:198371/139‑1 (MQ=255)
                                                                aaaTGCTCATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                                                                 <  7:380593/139‑1 (MQ=255)
                                                                       cATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCAGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGATTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaagactgtca                                                          >  8:6450/1‑133 (MQ=255)
                                                                        aTCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGAc                                                           >  7:55437/1‑137 (MQ=255)
                                                                                ggcTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTGTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAg                                                 <  8:302355/137‑1 (MQ=255)
                                                                                                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                             <  8:244255/135‑1 (MQ=255)
                                                                                                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                             <  7:242440/135‑1 (MQ=255)
                                                                                                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                             <  1:399066/135‑1 (MQ=255)
                                                                                                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaaa                   <  6:15404/139‑1 (MQ=255)
                                                                                                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                    <  7:45242/138‑1 (MQ=255)
                                                                                                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                    <  8:55437/138‑1 (MQ=255)
                                                                                                                     aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa            <  1:435073/139‑1 (MQ=255)
                                                                                                                     aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa            >  4:116589/1‑139 (MQ=255)
                                                                                                                        ccGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                                                                 <  3:40207/83‑1 (MQ=255)
                                                                                                                        ccGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaag                                                                 >  4:40207/1‑83 (MQ=255)
                                                                                                                               caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg  <  1:196375/139‑1 (MQ=255)
                                                                                                                               caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg  <  3:116589/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
AACTCATTTTTGAGTATCTCCATTGCAGCCTCTGTAAAACGCTTGCTTAGCTGTTCATCTTCTAAAATACTCATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAG  >  CP006881/2174994‑2175259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑