Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,197 A→C L289L (CTT→CTG)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,1970AC95.7% 147.1 / ‑5.3 47L289L (CTT→CTG) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (23/22);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACA  >  CP006881/2175065‑2175323
                                                                                                                                    |                                                                                                                              
cATCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCAGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGATTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCaagactgtca                                                                                                                          >  8:6450/1‑133 (MQ=255)
 aTCGCGCGGGCTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGAc                                                                                                                           >  7:55437/1‑137 (MQ=255)
         ggcTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTGTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAg                                                                                                                 <  8:302355/137‑1 (MQ=255)
                              accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                             <  8:244255/135‑1 (MQ=255)
                              accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                             <  1:399066/135‑1 (MQ=255)
                              accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                             <  7:242440/135‑1 (MQ=255)
                                       caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaaa                                                                                   <  6:15404/139‑1 (MQ=255)
                                       caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                    <  8:55437/138‑1 (MQ=255)
                                       caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                    <  7:45242/138‑1 (MQ=255)
                                              aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa                                                                            <  1:435073/139‑1 (MQ=255)
                                              aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa                                                                            >  4:116589/1‑139 (MQ=255)
                                                        caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                  <  1:196375/139‑1 (MQ=255)
                                                        caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                  <  3:116589/139‑1 (MQ=255)
                                                                       ccTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAaca                                                        <  6:63682/134‑1 (MQ=255)
                                                                       ccTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAaca                                                        >  5:63682/1‑134 (MQ=255)
                                                                       ccTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTc                                                   >  1:262877/1‑139 (MQ=255)
                                                                           agggaTACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCtt                                                 <  1:365596/133‑1 (MQ=255)
                                                                           agggaTACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGa                                               >  2:407726/5‑139 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCCCTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                        >  3:62832/1‑139 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATaa                                                                    >  6:211236/1‑111 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAaca                                                        >  3:145276/1‑123 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                          >  8:387860/1‑137 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                        >  8:261649/1‑139 (MQ=255)
                                                                                  ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                        >  8:158078/1‑139 (MQ=255)
                                                                                             tgttCCAATACAAGGCACTCCGCACGCCCTCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACt                             >  4:211595/1‑139 (MQ=255)
                                                                                             tgttCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAATCTCTTCATCACGGTCTTGATTTCCAAGCATTAAGACt                             >  7:239500/1‑139 (MQ=255)
                                                                                             tgttCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACt                             >  1:39864/1‑139 (MQ=255)
                                                                                                tCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGAACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTgg                          >  1:208108/1‑139 (MQ=255)
                                                                                                   aaTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtct                       >  8:179793/2‑139 (MQ=255)
                                                                                                    aTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                          >  1:107232/1‑119 (MQ=255)
                                                                                                    aTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                          <  2:107232/119‑1 (MQ=255)
                                                                                                     tACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttc                     >  5:55305/1‑139 (MQ=255)
                                                                                                      aCAAGGCACTCCGCGAGCCATCGCGTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCATCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttcc                    <  6:138452/139‑2 (MQ=255)
                                                                                                       cAAGGCACTCCGCAAGCCATCGCTTCAAGCAGAACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttcc                    <  2:208108/138‑2 (MQ=255)
                                                                                                         aGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                  <  4:32383/90‑1 (MQ=255)
                                                                                                          ggCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTg                           <  4:400499/128‑1 (MQ=255)
                                                                                                          ggCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTg                           >  3:400499/1‑128 (MQ=255)
                                                                                                               tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCa                                         <  8:74951/109‑1 (MQ=255)
                                                                                                               tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCa                                         >  7:74951/1‑109 (MQ=255)
                                                                                                               tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat           >  8:151358/1‑139 (MQ=255)
                                                                                                               tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat           >  6:284017/1‑139 (MQ=255)
                                                                                                               tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat           >  4:4755/1‑139 (MQ=255)
                                                                                                                 cgcaAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                    <  4:196312/62‑1 (MQ=255)
                                                                                                                 cgcaAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCATATCGCTAATAGAATAAAg                                                                  <  4:68101/80‑1 (MQ=255)
                                                                                                                       ccATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat   <  2:262877/139‑2 (MQ=255)
                                                                                                                        cATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGata  <  6:143029/139‑3 (MQ=255)
                                                                                                                        cATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTATCAGATAACAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGata  <  4:162694/139‑3 (MQ=255)
                                                                                                                         aTCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat   >  4:182342/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                              
CATCGCGCGGTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACA  >  CP006881/2175065‑2175323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑