Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,209 G→A F285F (TTC→TTT)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,2090GA96.3% 154.9 / ‑2.5 54F285F (TTC→TTT) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (27/25);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTT  >  CP006881/2175074‑2175343
                                                                                                                                       |                                                                                                                                      
ggcTGTTGCGGCCGTAACATCACCGACATCCACCAAAAATCCGCTCACATTGTGTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAg                                                                                                                                     <  8:302355/137‑1 (MQ=255)
                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                                                 <  7:242440/135‑1 (MQ=255)
                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                                                 <  8:244255/135‑1 (MQ=255)
                     accgACATCCACCAAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGAtagcag                                                                                                                 <  1:399066/135‑1 (MQ=255)
                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaaa                                                                                                       <  6:15404/139‑1 (MQ=255)
                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                                        <  7:45242/138‑1 (MQ=255)
                              caccaAAAATCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                                        <  8:55437/138‑1 (MQ=255)
                                     aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa                                                                                                >  4:116589/1‑139 (MQ=255)
                                     aaTCCGCTCACATTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTaa                                                                                                <  1:435073/139‑1 (MQ=255)
                                               caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                                      <  1:196375/139‑1 (MQ=255)
                                               caTTGTTTTTTATAACCTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                                      <  3:116589/139‑1 (MQ=255)
                                                              ccTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAaca                                                                            >  5:63682/1‑134 (MQ=255)
                                                              ccTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAaca                                                                            <  6:63682/134‑1 (MQ=255)
                                                              ccTCAGGGATACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTc                                                                       >  1:262877/1‑139 (MQ=255)
                                                                  agggaTACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCtt                                                                     <  1:365596/133‑1 (MQ=255)
                                                                  agggaTACCGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGa                                                                   >  2:407726/5‑139 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCCCTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                                            >  3:62832/1‑139 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATaa                                                                                        >  6:211236/1‑111 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAaca                                                                            >  3:145276/1‑123 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                                              >  8:387860/1‑137 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                                            >  8:261649/1‑139 (MQ=255)
                                                                         ccGCCAATGTTTGTTCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCaa                                                            >  8:158078/1‑139 (MQ=255)
                                                                                    tgttCCAATACAAGGCACTCCGCACGCCCTCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACt                                                 >  4:211595/1‑139 (MQ=255)
                                                                                    tgttCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAATCTCTTCATCACGGTCTTGATTTCCAAGCATTAAGACt                                                 >  7:239500/1‑139 (MQ=255)
                                                                                    tgttCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACt                                                 >  1:39864/1‑139 (MQ=255)
                                                                                       tCCAATACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGAACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTgg                                              >  1:208108/1‑139 (MQ=255)
                                                                                          aaTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtct                                           >  8:179793/2‑139 (MQ=255)
                                                                                           aTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                                              >  1:107232/1‑119 (MQ=255)
                                                                                           aTACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTcc                                                              <  2:107232/119‑1 (MQ=255)
                                                                                            tACAAGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttc                                         >  5:55305/1‑139 (MQ=255)
                                                                                             aCAAGGCACTCCGCGAGCCATCGCGTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCATCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttcc                                        <  6:138452/139‑2 (MQ=255)
                                                                                              cAAGGCACTCCGCAAGCCATCGCTTCAAGCAGAACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGtcttcc                                        <  2:208108/138‑2 (MQ=255)
                                                                                                aGGCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAg                                                                                      <  4:32383/90‑1 (MQ=255)
                                                                                                 ggCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTg                                               >  3:400499/1‑128 (MQ=255)
                                                                                                 ggCACTCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTg                                               <  4:400499/128‑1 (MQ=255)
                                                                                                      tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCa                                                             <  8:74951/109‑1 (MQ=255)
                                                                                                      tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCa                                                             >  7:74951/1‑109 (MQ=255)
                                                                                                      tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat                               >  4:4755/1‑139 (MQ=255)
                                                                                                      tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat                               >  6:284017/1‑139 (MQ=255)
                                                                                                      tCCGCAAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCatat                               >  8:151358/1‑139 (MQ=255)
                                                                                                        cgcaAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCaa                                                                                                        <  4:196312/62‑1 (MQ=255)
                                                                                                        cgcaAGCCATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCATATCGCTAATAGAATAAAg                                                                                      <  4:68101/80‑1 (MQ=255)
                                                                                                              ccATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat                       <  2:262877/139‑2 (MQ=255)
                                                                                                               cATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGata                      <  6:143029/139‑3 (MQ=255)
                                                                                                               cATCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTATCAGATAACAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGata                      <  4:162694/139‑3 (MQ=255)
                                                                                                                aTCGCTTCAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat                       >  4:182342/1‑136 (MQ=255)
                                                                                                                         agcagGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAgg            <  7:179793/136‑1 (MQ=255)
                                                                                                                              gACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat                       <  8:137068/123‑2 (MQ=255)
                                                                                                                              gACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGat                       >  7:137068/1‑122 (MQ=255)
                                                                                                                              gACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTc       >  7:76905/1‑139 (MQ=255)
                                                                                                                               aCAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTc       >  1:99942/1‑138 (MQ=255)
                                                                                                                                cAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGa     <  2:39864/139‑1 (MQ=255)
                                                                                                                                   ggCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGAttt  >  8:410469/1‑139 (MQ=255)
                                                                                                                                   ggCCAAAGCTATCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGAttt  >  6:155535/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GTCTGTTGCGGCTGCAACGTCGCTGACATCCACCAAAAATCCGCTCACATTGTTCTTAATAACCTCCGGAATACCGCCAATGTTTGTTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTT  >  CP006881/2175074‑2175343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑