Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,347	T→C	P239P (CCA→CCG)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,347	0	T	C	100.0%	174.9 / NA	54	P239P (CCA→CCG)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/27);  total (27/27)

GCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTG  >  CP006881/2175212‑2175474                                                                                                                                        |                                                                                                                                gCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATCAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGa                                                                                                                              <  1:160103/139‑1 (MQ=255)         tttCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGTTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGtctc                                                                                                                       <  6:55305/138‑1 (MQ=255)         tttCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGtctc                                                                                                                       <  7:387860/138‑1 (MQ=255)         tttCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGtctc                                                                                                                       <  3:4755/138‑1 (MQ=255)           tCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAATACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCAccgc                                                                                                                    >  5:45931/1‑137 (MQ=255)            cAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGAc                                                                                                                   >  1:160818/1‑139 (MQ=255)                           tcaaaTCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGc                                                                                                    >  3:425177/3‑139 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTccga                                                                                                                    >  4:266331/1‑113 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTccga                                                                                                                    <  3:266331/115‑3 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                            >  8:436693/1‑139 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                            >  1:11540/1‑139 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                            >  5:25402/1‑139 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                            >  3:438252/1‑139 (MQ=255)                                   cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCGTTCGttt                                                                                            >  3:33970/1‑139 (MQ=255)                                      atagAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGc                                                                                         <  7:410469/136‑1 (MQ=255)                                         gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTCAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTATAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      <  1:30528/139‑3 (MQ=255)                                         gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      <  7:151358/139‑3 (MQ=255)                                         gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      <  7:158078/139‑3 (MQ=255)                                         gAATAAAGATCTGCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      <  3:211595/139‑3 (MQ=255)                                                    ttcAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCg                                                                            >  7:14884/2‑138 (MQ=255)                                                    ttcAACACGGTCTTGATTTCAAAGAATTAAGACTTGGTCTTAAAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCGTTAGTTTTGCCAGCGATATTGct                                                                            >  7:21319/2‑137 (MQ=255)                                                      cAACCCGTTCTTTATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCCACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGc                                                                             <  4:62832/135‑1 (MQ=255)                                                      cAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGACAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGaa                                                                         >  4:199431/1‑139 (MQ=255)                                                       aaCACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGaa                                                                         <  4:438252/138‑1 (MQ=255)                                                       aaCACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAac                                                                        <  5:175198/139‑1 (MQ=255)                                                        acacGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAaca                                                                       <  3:182342/139‑1 (MQ=255)                                                         cacGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacac                                                                      <  1:407726/139‑1 (MQ=255)                                                          acGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGACAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacaca                                                                     <  3:199431/139‑1 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGa                                                                          >  5:256433/1‑133 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGa                                                                          <  6:256433/133‑1 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    <  7:261649/139‑1 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    <  3:168207/139‑1 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    <  8:14884/139‑1 (MQ=255)                                                           cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    <  8:21319/139‑1 (MQ=255)                                                            ggTCTTGATTTCCAAGCATTAAGACTTGTTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCTATTTCTCCGGACCGTGTCCGACTAAAAGAAGCTTCGCTTTCGGTTTGCCAGCGATTTTGAGGAacaaacc                                                                   >  8:114409/1‑138 (MQ=255)                                                              tCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGGCAGCGCTATTGCGGAACCCCCGGa                                                                 >  4:84335/1‑139 (MQ=255)                                                              tCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCAAATTTTCTCATAAGCTCGCACGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGGTTTGGCAGAGATAATGCCGAACACCCggc                                                                 >  6:175198/1‑138 (MQ=255)                                                              tCTTGATTTCCAAGCATTAAGACTTGCTCTAACAACCCAAATTTTCTGATAAGATAGCAGGCAGTCGAATTCTCCGGACCGTCTCCGACTAAAAGCACCATCGCTTTCGTTTTTCCATCGAAATTTCGGAACACACGGa                                                                 >  6:396953/1‑139 (MQ=255)                                                                  gATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGGCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGAt                                                                <  3:379492/136‑1 (MQ=255)                                                                   aTTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGAca                                                            <  5:284017/139‑2 (MQ=255)                                                                        cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    <  6:140370/126‑1 (MQ=255)                                                                        cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                    >  5:140370/1‑126 (MQ=255)                                                                        cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTg                                                       >  7:20544/1‑139 (MQ=255)                                                                        cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTg                                                       >  4:98120/1‑139 (MQ=255)                                                                                       ggTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      >  6:438981/1‑91 (MQ=255)                                                                                       ggTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                      <  5:438981/93‑3 (MQ=255)                                                                                                   cATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGa                            <  3:98120/139‑1 (MQ=255)                                                                                                     tatTTTCTGATAAGCTCGCAGTCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGGCAGCGAGATTGGGGAAAACACGGATGACATCCTGCACGCGTTTAACTTTTATGAAGttttcaa                          >  3:440703/1‑134 (MQ=255)                                                                                                       ttttCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTTCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGAcaca                        >  6:182932/1‑139 (MQ=255)                                                                                                            tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                   >  7:154162/1‑139 (MQ=255)                                                                                                            tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                   >  3:62222/1‑139 (MQ=255)                                                                                                                   cTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACt            >  1:23966/1‑139 (MQ=255)                                                                                                                           cTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATc    <  3:84335/139‑1 (MQ=255)                                                                                                                             gTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCCCGCGTTTAACTTTTCTGAAGTTGGACCCATGGATGACGGCTTTTTCATCTg  >  8:224459/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                GCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTG  >  CP006881/2175212‑2175474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑