Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,359 A→G V235V (GTT→GTC)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,3590AG98.2% 172.3 / NA 54V235V (GTT→GTC) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (28/25);  minor base C (0/1);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCT  >  CP006881/2175223‑2175492
                                                                                                                                        |                                                                                                                                     
cAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGAc                                                                                                                                     >  1:160818/1‑139 (MQ=255)
               tcaaaTCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGc                                                                                                                      >  3:425177/3‑139 (MQ=255)
                       cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                                              >  8:436693/1‑139 (MQ=255)
                       cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                                              >  1:11540/1‑139 (MQ=255)
                       cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                                              >  5:25402/1‑139 (MQ=255)
                       cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGttt                                                                                                              >  3:438252/1‑139 (MQ=255)
                       cTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCGTTCGttt                                                                                                              >  3:33970/1‑139 (MQ=255)
                          atagAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGc                                                                                                           <  7:410469/136‑1 (MQ=255)
                             gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTCAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTATAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        <  1:30528/139‑3 (MQ=255)
                             gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        <  7:151358/139‑3 (MQ=255)
                             gAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        <  7:158078/139‑3 (MQ=255)
                             gAATAAAGATCTGCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        <  3:211595/139‑3 (MQ=255)
                                        ttcAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCg                                                                                              >  7:14884/2‑138 (MQ=255)
                                        ttcAACACGGTCTTGATTTCAAAGAATTAAGACTTGGTCTTAAAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCGTTAGTTTTGCCAGCGATATTGct                                                                                              >  7:21319/2‑137 (MQ=255)
                                          cAACCCGTTCTTTATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCCACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGc                                                                                               <  4:62832/135‑1 (MQ=255)
                                          cAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGACAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGaa                                                                                           >  4:199431/1‑139 (MQ=255)
                                           aaCACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGaa                                                                                           <  4:438252/138‑1 (MQ=255)
                                           aaCACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAac                                                                                          <  5:175198/139‑1 (MQ=255)
                                            acacGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAaca                                                                                         <  3:182342/139‑1 (MQ=255)
                                             cacGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacac                                                                                        <  1:407726/139‑1 (MQ=255)
                                              acGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGACAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacaca                                                                                       <  3:199431/139‑1 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGa                                                                                            >  5:256433/1‑133 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGa                                                                                            <  6:256433/133‑1 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      <  7:261649/139‑1 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      <  8:14884/139‑1 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      <  8:21319/139‑1 (MQ=255)
                                               cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      <  3:168207/139‑1 (MQ=255)
                                                ggTCTTGATTTCCAAGCATTAAGACTTGTTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCTATTTCTCCGGACCGTGTCCGACTAAAAGAAGCTTCGCTTTCGGTTTGCCAGCGATTTTGAGGAacaaacc                                                                                     >  8:114409/1‑138 (MQ=255)
                                                  tCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGGCAGCGCTATTGCGGAACCCCCGGa                                                                                   >  4:84335/1‑139 (MQ=255)
                                                  tCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCAAATTTTCTCATAAGCTCGCACGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGGTTTGGCAGAGATAATGCCGAACACCCggc                                                                                   >  6:175198/1‑138 (MQ=255)
                                                  tCTTGATTTCCAAGCATTAAGACTTGCTCTAACAACCCAAATTTTCTGATAAGATAGCAGGCAGTCGAATTCTCCGGACCGTCTCCGACTAAAAGCACCATCGCTTTCGTTTTTCCATCGAAATTTCGGAACACACGGa                                                                                   >  6:396953/1‑139 (MQ=255)
                                                      gATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGGCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGAt                                                                                  <  3:379492/136‑1 (MQ=255)
                                                       aTTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGAca                                                                              <  5:284017/139‑2 (MQ=255)
                                                            cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      <  6:140370/126‑1 (MQ=255)
                                                            cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                                                                                      >  5:140370/1‑126 (MQ=255)
                                                            cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTg                                                                         >  4:98120/1‑139 (MQ=255)
                                                            cAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTg                                                                         >  7:20544/1‑139 (MQ=255)
                                                                           ggTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        >  6:438981/1‑91 (MQ=255)
                                                                           ggTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGccag                                                                                                        <  5:438981/93‑3 (MQ=255)
                                                                                       cATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGa                                              <  3:98120/139‑1 (MQ=255)
                                                                                         tatTTTCTGATAAGCTCGCAGTCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGGCAGCGAGATTGGGGAAAACACGGATGACATCCTGCACGCGTTTAACTTTTATGAAGttttcaa                                            >  3:440703/1‑134 (MQ=255)
                                                                                           ttttCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTTCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGAcaca                                          >  6:182932/1‑139 (MQ=255)
                                                                                                tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                                     >  7:154162/1‑139 (MQ=255)
                                                                                                tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                                     >  3:62222/1‑139 (MQ=255)
                                                                                                       cTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACt                              >  1:23966/1‑139 (MQ=255)
                                                                                                               cTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATc                      <  3:84335/139‑1 (MQ=255)
                                                                                                                 gTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCCCGCGTTTAACTTTTCTGAAGTTGGACCCATGGATGACGGCTTTTTCATCTg                    >  8:224459/1‑139 (MQ=255)
                                                                                                                            cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAGGACGACTTTTTCATCTGGTAAAAtt            <  4:425177/135‑2 (MQ=255)
                                                                                                                            cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATGCATCACGACTTTTTCATCTGGTAAAATTcc          <  7:436693/137‑1 (MQ=255)
                                                                                                                                   tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCTGCGATATTGCGGAACACACGGATg                                                                                 >  8:69208/1‑60 (MQ=255)
                                                                                                                                   tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCTGCGATATTGCGGAACACACGGATg                                                                                 <  7:69208/60‑1 (MQ=255)
                                                                                                                                   tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGGGGAACACCCGGATGACAACCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATTGATGACGACTTTTTCATCTGGTAAAATTCCATGTTtct  >  6:333953/1‑139 (MQ=255)
                                                                                                                                   tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGACCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                 <  7:359582/124‑1 (MQ=255)
                                                                                                                                   tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                 >  8:359582/1‑124 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCT  >  CP006881/2175223‑2175492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑