Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,425 A→G R213R (CGT→CGC)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,4250AG97.3% 113.7 / NA 37R213R (CGT→CGC) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/19);  minor base C (1/0);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGAT  >  CP006881/2175310‑2175559
                                                                                                                   |                                                                                                                                      
cATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGa                                                                                                                 <  3:98120/139‑1 (MQ=255)
  tatTTTCTGATAAGCTCGCAGTCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGGCAGCGAGATTGGGGAAAACACGGATGACATCCTGCACGCGTTTAACTTTTATGAAGttttcaa                                                                                                               >  3:440703/1‑134 (MQ=255)
    ttttCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTTCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGAcaca                                                                                                             >  6:182932/1‑139 (MQ=255)
         tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                                                                                                        >  7:154162/1‑139 (MQ=255)
         tGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAt                                                                                                        >  3:62222/1‑139 (MQ=255)
                cTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACt                                                                                                 >  1:23966/1‑139 (MQ=255)
                        cTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATc                                                                                         <  3:84335/139‑1 (MQ=255)
                          gTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCCCGCGTTTAACTTTTCTGAAGTTGGACCCATGGATGACGGCTTTTTCATCTg                                                                                       >  8:224459/1‑139 (MQ=255)
                                     cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAGGACGACTTTTTCATCTGGTAAAAtt                                                                               <  4:425177/135‑2 (MQ=255)
                                     cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATGCATCACGACTTTTTCATCTGGTAAAATTcc                                                                             <  7:436693/137‑1 (MQ=255)
                                            tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGGGGAACACCCGGATGACAACCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATTGATGACGACTTTTTCATCTGGTAAAATTCCATGTTtct                                                                     >  6:333953/1‑139 (MQ=255)
                                            tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGACCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                    <  7:359582/124‑1 (MQ=255)
                                            tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                    >  8:359582/1‑124 (MQ=255)
                                                   cTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAAt                                                              <  5:182932/139‑1 (MQ=255)
                                                       aaGCAGCTTCGCTTTCGTTTTGCCCGCGATATTGCGGAACACCCCGATGACATCCTGCACCCGTTTAACGTTTCTGAAGTTGGACACATGGATGACGACATTTTAATCTGGTAAAAATCCATGTTTCTCTTTAATTGcc                                                          >  1:370326/1‑139 (MQ=255)
                                                        agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                         <  6:45931/139‑1 (MQ=255)
                                                        agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                         <  6:25402/139‑1 (MQ=255)
                                                                       gTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTttcttc                                           <  4:62222/138‑1 (MQ=255)
                                                                                 cGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACAcgcg                                <  8:154162/139‑1 (MQ=255)
                                                                                   atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  5:122802/139‑1 (MQ=255)
                                                                                   atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  8:20544/139‑1 (MQ=255)
                                                                                   atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  4:440703/139‑1 (MQ=255)
                                                                                   atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  2:23966/139‑1 (MQ=255)
                                                                                   atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  2:11540/139‑1 (MQ=255)
                                                                                    tatTGCGGAACAAACGGATGAGATCCTGCACGCGTTTAACTTTTCTGCAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTc                             <  2:403696/139‑1 (MQ=255)
                                                                                           gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtata                      <  2:160818/139‑1 (MQ=255)
                                                                                           gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtat                       >  8:149380/1‑138 (MQ=255)
                                                                                            aaCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATaa                     <  4:423758/139‑1 (MQ=255)
                                                                                              cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              >  5:400329/1‑128 (MQ=255)
                                                                                              cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                              <  6:400329/128‑1 (MQ=255)
                                                                                              cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAg                   >  2:206207/1‑139 (MQ=255)
                                                                                                      tGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATTAAGTTATAAAt           >  6:49436/1‑139 (MQ=255)
                                                                                                        acatCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCg         >  1:105934/4‑139 (MQ=255)
                                                                                                          atCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACCCGCGCTCGTCTATAAAGTAATAAATCGtt       >  5:119585/2‑139 (MQ=255)
                                                                                                           tCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGtt       >  3:106276/1‑138 (MQ=255)
                                                                                                              tGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTcaa   >  1:237318/1‑137 (MQ=255)
                                                                                                               gCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAAt  >  8:392286/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                      
CATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGAT  >  CP006881/2175310‑2175559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑