Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,461	A→G	V201V (GTT→GTC)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,461	0	A	G	100.0%	163.4 / NA	51	V201V (GTT→GTC)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

CTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGATTTTTTTCTCCGGTTTAATTAAATCGTA  >  CP006881/2175326‑2175586                                                                                                                                        |                                                                                                                              cTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACt                                                                                                                            >  1:23966/1‑139 (MQ=255)         cTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATc                                                                                                                    <  3:84335/139‑1 (MQ=255)           gTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCCCGCGTTTAACTTTTCTGAAGTTGGACCCATGGATGACGGCTTTTTCATCTg                                                                                                                  >  8:224459/1‑139 (MQ=255)                      cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAGGACGACTTTTTCATCTGGTAAAAtt                                                                                                          <  4:425177/135‑2 (MQ=255)                      cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATGCATCACGACTTTTTCATCTGGTAAAATTcc                                                                                                        <  7:436693/137‑1 (MQ=255)                             tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGGGGAACACCCGGATGACAACCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATTGATGACGACTTTTTCATCTGGTAAAATTCCATGTTtct                                                                                                >  6:333953/1‑139 (MQ=255)                             tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGACCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                                               <  7:359582/124‑1 (MQ=255)                             tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                                               >  8:359582/1‑124 (MQ=255)                                    cTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAAt                                                                                         <  5:182932/139‑1 (MQ=255)                                        aaGCAGCTTCGCTTTCGTTTTGCCCGCGATATTGCGGAACACCCCGATGACATCCTGCACCCGTTTAACGTTTCTGAAGTTGGACACATGGATGACGACATTTTAATCTGGTAAAAATCCATGTTTCTCTTTAATTGcc                                                                                     >  1:370326/1‑139 (MQ=255)                                         agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                                                    <  6:25402/139‑1 (MQ=255)                                         agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                                                    <  6:45931/139‑1 (MQ=255)                                                        gTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTttcttc                                                                      <  4:62222/138‑1 (MQ=255)                                                                  cGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACAcgcg                                                           <  8:154162/139‑1 (MQ=255)                                                                    atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  2:23966/139‑1 (MQ=255)                                                                    atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  5:122802/139‑1 (MQ=255)                                                                    atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  2:11540/139‑1 (MQ=255)                                                                    atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  8:20544/139‑1 (MQ=255)                                                                    atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  4:440703/139‑1 (MQ=255)                                                                     tatTGCGGAACAAACGGATGAGATCCTGCACGCGTTTAACTTTTCTGCAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTc                                                        <  2:403696/139‑1 (MQ=255)                                                                            gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtata                                                 <  2:160818/139‑1 (MQ=255)                                                                            gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtat                                                  >  8:149380/1‑138 (MQ=255)                                                                             aaCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATaa                                                <  4:423758/139‑1 (MQ=255)                                                                               cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         >  5:400329/1‑128 (MQ=255)                                                                               cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  6:400329/128‑1 (MQ=255)                                                                               cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAg                                              >  2:206207/1‑139 (MQ=255)                                                                                       tGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATTAAGTTATAAAt                                      >  6:49436/1‑139 (MQ=255)                                                                                         acatCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCg                                    >  1:105934/4‑139 (MQ=255)                                                                                           atCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACCCGCGCTCGTCTATAAAGTAATAAATCGtt                                  >  5:119585/2‑139 (MQ=255)                                                                                            tCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGtt                                  >  3:106276/1‑138 (MQ=255)                                                                                               tGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTcaa                              >  1:237318/1‑137 (MQ=255)                                                                                                gCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAAt                             >  8:392286/1‑139 (MQ=255)                                                                                                       tttAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTtc                      >  5:344822/1‑139 (MQ=255)                                                                                                         taacttttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTtct                     <  5:155535/133‑1 (MQ=255)                                                                                                              tttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGtt               >  2:24205/1‑139 (MQ=255)                                                                                                               ttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGtt               <  2:105934/138‑1 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCa                                                                     >  5:256722/1‑83 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCa                                                                     <  6:256722/83‑1 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt              >  7:381786/1‑138 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt              >  8:431626/1‑138 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt              >  1:263139/1‑138 (MQ=255)                                                                                                                tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt              >  6:68906/1‑138 (MQ=255)                                                                                                                  tgaaGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTttaat           >  2:145232/3‑139 (MQ=255)                                                                                                                    aaGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTttaatt          <  8:381786/138‑1 (MQ=255)                                                                                                                      gTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTaaa       <  3:58002/139‑1 (MQ=255)                                                                                                                         ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAAt                                                                                         <  2:37926/54‑1 (MQ=255)                                                                                                                         ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         >  2:359918/1‑86 (MQ=255)                                                                                                                         ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                         <  1:359918/86‑1 (MQ=255)                                                                                                                           acacATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTa  <  5:68906/139‑1 (MQ=255)                                                                                                                                    tGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGt   >  3:71677/1‑129 (MQ=255)                                                                                                                                    tGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGt   <  4:71677/129‑1 (MQ=255)                                                                                                                                        |                                                                                                                              CTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGATTTTTTTCTCCGGTTTAATTAAATCGTA  >  CP006881/2175326‑2175586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑