Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,175,470	G→A	D198D (GAC→GAT)	U712_10880 ←	putative glycosyltransferase ypjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,175,470	0	G	A	100.0%	168.4 / NA	55	D198D (GAC→GAT)	U712_10880	putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

CTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGATTTTTTTCTCCGGTTTAATTAAATCGTACGTTTCAGCTGC  >  CP006881/2175334‑2175598                                                                                                                                         |                                                                                                                                 cTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATc                                                                                                                                <  3:84335/139‑1 (MQ=255)   gTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCCCGCGTTTAACTTTTCTGAAGTTGGACCCATGGATGACGGCTTTTTCATCTg                                                                                                                              >  8:224459/1‑139 (MQ=255)              cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGAGGACGACTTTTTCATCTGGTAAAAtt                                                                                                                      <  4:425177/135‑2 (MQ=255)              cggACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATGCATCACGACTTTTTCATCTGGTAAAATTcc                                                                                                                    <  7:436693/137‑1 (MQ=255)                     tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGGGGAACACCCGGATGACAACCTGCACGCGGTTAACTTTTCTGAAGTTGGACACATTGATGACGACTTTTTCATCTGGTAAAATTCCATGTTtct                                                                                                            >  6:333953/1‑139 (MQ=255)                     tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGACCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                                                           <  7:359582/124‑1 (MQ=255)                     tctcCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTa                                                                                                                           >  8:359582/1‑124 (MQ=255)                            cTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAAt                                                                                                     <  5:182932/139‑1 (MQ=255)                                aaGCAGCTTCGCTTTCGTTTTGCCCGCGATATTGCGGAACACCCCGATGACATCCTGCACCCGTTTAACGTTTCTGAAGTTGGACACATGGATGACGACATTTTAATCTGGTAAAAATCCATGTTTCTCTTTAATTGcc                                                                                                 >  1:370326/1‑139 (MQ=255)                                 agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                                                                <  6:25402/139‑1 (MQ=255)                                 agcagcTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCg                                                                                                <  6:45931/139‑1 (MQ=255)                                                gTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTttcttc                                                                                  <  4:62222/138‑1 (MQ=255)                                                          cGATATTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACAcgcg                                                                       <  8:154162/139‑1 (MQ=255)                                                            atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  5:122802/139‑1 (MQ=255)                                                            atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  2:23966/139‑1 (MQ=255)                                                            atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  4:440703/139‑1 (MQ=255)                                                            atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  2:11540/139‑1 (MQ=255)                                                            atatTGCGGAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  8:20544/139‑1 (MQ=255)                                                             tatTGCGGAACAAACGGATGAGATCCTGCACGCGTTTAACTTTTCTGCAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTc                                                                    <  2:403696/139‑1 (MQ=255)                                                                    gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtata                                                             <  2:160818/139‑1 (MQ=255)                                                                    gAACACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCtat                                                              >  8:149380/1‑138 (MQ=255)                                                                     aaCACACGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATaa                                                            <  4:423758/139‑1 (MQ=255)                                                                       cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  6:400329/128‑1 (MQ=255)                                                                       cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     >  5:400329/1‑128 (MQ=255)                                                                       cacacGGATGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAg                                                          >  2:206207/1‑139 (MQ=255)                                                                               tGACATCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATTAAGTTATAAAt                                                  >  6:49436/1‑139 (MQ=255)                                                                                 acatCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCg                                                >  1:105934/4‑139 (MQ=255)                                                                                   atCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACCCGCGCTCGTCTATAAAGTAATAAATCGtt                                              >  5:119585/2‑139 (MQ=255)                                                                                    tCCTGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGtt                                              >  3:106276/1‑138 (MQ=255)                                                                                       tGCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTcaa                                          >  1:237318/1‑137 (MQ=255)                                                                                        gCACGCGTTTAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAAt                                         >  8:392286/1‑139 (MQ=255)                                                                                               tttAACTTTTCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTtc                                  >  5:344822/1‑139 (MQ=255)                                                                                                 taacttttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTtct                                 <  5:155535/133‑1 (MQ=255)                                                                                                      tttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGtt                           >  2:24205/1‑139 (MQ=255)                                                                                                       ttCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGtt                           <  2:105934/138‑1 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCa                                                                                 >  5:256722/1‑83 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCa                                                                                 <  6:256722/83‑1 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt                          >  8:431626/1‑138 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt                          >  1:263139/1‑138 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt                          >  7:381786/1‑138 (MQ=255)                                                                                                        tCTGAAGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGttt                          >  6:68906/1‑138 (MQ=255)                                                                                                          tgaaGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTttaat                       >  2:145232/3‑139 (MQ=255)                                                                                                            aaGTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTttaatt                      <  8:381786/138‑1 (MQ=255)                                                                                                              gTTGGACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTaaa                   <  3:58002/139‑1 (MQ=255)                                                                                                                 ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAAt                                                                                                     <  2:37926/54‑1 (MQ=255)                                                                                                                 ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     >  2:359918/1‑86 (MQ=255)                                                                                                                 ggACACATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCt                                                                     <  1:359918/86‑1 (MQ=255)                                                                                                                   acacATGGATGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTa              <  5:68906/139‑1 (MQ=255)                                                                                                                            tGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGt               >  3:71677/1‑129 (MQ=255)                                                                                                                            tGACGACTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGt               <  4:71677/129‑1 (MQ=255)                                                                                                                               cgaCTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTATGTTTCAGCCGc  <  6:344822/137‑1 (MQ=255)                                                                                                                                 aCTTTTTCATCTGGTAAAATTCTATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTATGTTTCAGCCGc  >  3:350535/1‑137 (MQ=255)                                                                                                                                 aCTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTATGTTTCAGCCGc  >  6:419424/1‑137 (MQ=255)                                                                                                                                 aCTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTATGTTTCAGCCGc  >  2:366856/1‑137 (MQ=255)                                                                                                                                 aCTTTTTCATCTGGTAAAATTCCATGTTTCTCTTTAATTGCCGCTGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCAATTTTTTTCTCCGGTTTAATTAAATCGTATGTTTCAGCCGc  >  4:432375/1‑137 (MQ=255)                                                                                                                                         |                                                                                                                                 CTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACGTTTGACCTTTCTAAAGTTGGACACATGGATGACAACTTTATCGTCTGGTAAAATACCATGTTTCTCTTTAATTGCCGCAGTGTTTTTCTTCAGATACACGCGCTCGTCTATAAAGTTATAAATCGTTTCGATTTTTTTCTCCGGTTTAATTAAATCGTACGTTTCAGCTGC  >  CP006881/2175334‑2175598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑