Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,175,914 G→A H50H (CAC→CAT)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,9140GA98.2% 170.3 / NA 55H50H (CAC→CAT) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/29);  minor base C (0/1);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCATGGATAATGTCTAAATTCTCCCGTTCTGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCGTATTGATTAACCTCAACTTCATGAAAATGAATATTGGGGTGATATGTATTTAGTCTAAACGGAATGCTTGACGTAATAAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCGAGTTCTGTCGCAATGATACCTGAGCCTCCGACGCTCGGATAGCATGTGATCCCTAT  >  CP006881/2175783‑2176048
                                                                                                                                   |                                                                                                                                      
tGAGCATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATg                                                                                                                                 >  2:294207/1‑139 (MQ=255)
  aGCATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTa                                                                                                                               >  3:53769/1‑139 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGTTTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             <  5:135467/139‑1 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             <  8:315704/139‑1 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             <  8:269591/139‑1 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             <  2:250140/139‑1 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             <  8:366914/139‑1 (MQ=255)
    cATGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             >  7:123714/1‑139 (MQ=255)
     aTGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAttc                                                                                                                            <  7:62047/139‑2 (MQ=255)
     aTGGATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTAtt                                                                                                                             >  7:242705/1‑138 (MQ=255)
        gATAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGt                                                                                                                         >  1:400879/1‑139 (MQ=255)
          tAATGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTc                                                                                                                        >  8:335190/1‑138 (MQ=255)
            aTGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTa                                                                                                                      <  7:372588/138‑1 (MQ=255)
            aTGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTa                                                                                                                      >  4:97098/1‑138 (MQ=255)
            aTGTCTAAATTCTCCCGTTCCGACACCTCAGCGATTTTGATTGCCAATGTCAAATCATACGGAGGATACTTAAAAACACCATATTGATTAAACTCAAATTCATTAAAAAGAATATTCGGAATATATGTATTCAGTCTa                                                                                                                      >  6:135467/1‑138 (MQ=255)
             tGTCTAAATTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTaaa                                                                                                                    >  5:208075/1‑139 (MQ=255)
                  aaaTTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGaa                                                                                                               >  4:298187/1‑139 (MQ=255)
                   aaTTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACgg                                                                                                                 <  5:422221/136‑1 (MQ=255)
                   aaTTCTCCCGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGa                                                                                                                <  2:400879/137‑1 (MQ=255)
                          ccGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGat                                                                                                       <  7:335190/139‑2 (MQ=255)
                           cGTTCCGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGatg                                                                                                      <  6:68318/139‑3 (MQ=255)
                                   cACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATgaaa                                                                                              <  6:208075/139‑1 (MQ=255)
                                         aGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGa                                                                                          <  8:95013/137‑1 (MQ=255)
                                          gCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGAttt                                                                                       >  5:424419/1‑139 (MQ=255)
                                            gATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAATCGGAATGCTTGATGTGATGAAATGGATTTCa                                                                                     <  4:344190/139‑1 (MQ=255)
                                               tttGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACg                                                                                                                  <  1:290911/107‑1 (MQ=255)
                                               tttGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACg                                                                                                                  >  2:290911/1‑107 (MQ=255)
                                                                 aTCAGACGGAGGCTACTTAAAAACAGCATAGTGATTCACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAcctgc                                                                <  8:351972/139‑1 (MQ=255)
                                                                      aCGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTccc                                                           >  3:284683/1‑139 (MQ=255)
                                                                       cGGAGGATACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTccc                                                           <  4:53769/138‑1 (MQ=255)
                                                                           ggaTACTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGtt                                                      <  2:360047/139‑1 (MQ=255)
                                                                               aCTTAAAAACAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGt                                                  >  7:411443/1‑139 (MQ=255)
                                                                                   aaaaaCAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGc                                               <  5:166377/138‑1 (MQ=255)
                                                                                     aaaCAGCATATTGATTAAACTCAACATCATGAAAATGAATATTGGGATGATATGTATTCAGTCTACACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAAt                                            <  8:399249/139‑1 (MQ=255)
                                                                                      aaCAGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAAt                                            >  6:296010/1‑138 (MQ=255)
                                                                                         aGCATATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATa                                        >  2:131492/1‑139 (MQ=255)
                                                                                           catATTGATTAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAAGGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATAca                                      >  1:44048/3‑138 (MQ=255)
                                                                                                   ttAACCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTc                              >  8:208347/1‑139 (MQ=255)
                                                                                                     aaCCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATAcc                                      <  4:195381/129‑1 (MQ=255)
                                                                                                     aaCCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATAcc                                      >  3:195381/1‑129 (MQ=255)
                                                                                                     aaCCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCGTCACCAGTTCTGTCGCAATGATACGTGAGCCTcc                             >  2:376535/1‑138 (MQ=255)
                                                                                                      aCCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTccaa                           <  6:111349/139‑3 (MQ=255)
                                                                                                      aCCTCAACTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTccaa                           >  5:111349/1‑137 (MQ=255)
                                                                                                           aaCTTCATGTAAATGAATATGGGGATGATATGTATTCAGTCTAAACGGATTTCTAGATGTGATGAAATGGAGTTCATGTCCCTTTTCAGGAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCt                       <  5:139803/138‑1 (MQ=255)
                                                                                                           aaCTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCt                       <  3:298187/138‑1 (MQ=255)
                                                                                                           aaCTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCt                       <  8:411443/138‑1 (MQ=255)
                                                                                                            aCTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCg                     <  3:170079/139‑1 (MQ=255)
                                                                                                            aCTTCATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTCCATGTCCCTTTTCTGCAAGATGCTGCCCCAGTTCTGTCGCATTGATACCTGAGACGCCCAGGCTCg                     >  7:412410/1‑139 (MQ=255)
                                                                                                                cctGAAAATGAATAATGGGCAGATAAGTAGTCAGGCAAAACGGAATGCTTGCTGGGATGACATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCGGa                   <  6:259575/135‑1 (MQ=255)
                                                                                                                cATGAAAATGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCGGa                   >  1:349498/1‑137 (MQ=255)
                                                                                                                     aaaTGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCGGATAGCAt             <  5:191855/138‑1 (MQ=255)
                                                                                                                       aTGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGa                                   >  2:210995/1‑114 (MQ=255)
                                                                                                                       aTGAATATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGa                                   <  1:210995/114‑1 (MQ=255)
                                                                                                                          aaTATTGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCGGATAGCATGTGAtt       <  6:424419/139‑2 (MQ=255)
                                                                                                                               tGGGATGATATGTATTCAGTCTAAACGGAATGCTTGATGTGATGAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCCAGTTCTGTCGCAATGATACCTGAGCCTCCAACGCTCGGATAGCATGTGATTCCTAt  >  2:238093/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TGAGCATGGATAATGTCTAAATTCTCCCGTTCTGCCACCTCAGCGATTTTGCTTGCCAATGTCAAATCATACGGAGGATACTTAAAAACAGCGTATTGATTAACCTCAACTTCATGAAAATGAATATTGGGGTGATATGTATTTAGTCTAAACGGAATGCTTGACGTAATAAAATGGATTTCATGTCCCTTTTCAGCAAGCTGCTTCCCGAGTTCTGTCGCAATGATACCTGAGCCTCCGACGCTCGGATAGCATGTGATCCCTAT  >  CP006881/2175783‑2176048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑