Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,237,434	A→G	D559G (GAC→GGC)	U712_06215 →	PTS system mannose‑specific EIIBCA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,237,434	0	A	G	100.0%	243.0 / NA	75	D559G (GAC→GGC)	U712_06215	PTS system mannose‑specific EIIBCA component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/30);  total (45/30)

GAGTGATGACATCATAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGACATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGATTGCTG  >  CP006881/1237315‑1237568                                                                                                                        |                                                                                                                                       gAGTGATGACATCATAGATGAATTGAATCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTGTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCg                                                                                                                     <  1:8873/139‑1 (MQ=255)    tgatgaCATCATAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCTCTGCTTTCAGAGAGCGGGTATAACCAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCTATTGGCTTGAATATCGCGATTCAGCAc                                                                                                                  >  5:349537/1‑139 (MQ=255)       tgaCATCATAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCTTTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGa                                                                                                               <  8:120495/139‑1 (MQ=255)           atcatAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGt                                                                                                           >  6:52667/1‑139 (MQ=255)           atcatAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCACACGGAAAGt                                                                                                           >  1:416021/1‑139 (MQ=255)                aGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGa                                                                                                       <  3:100066/138‑1 (MQ=255)                     aaTTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCAc                                                                                                                  <  3:204658/122‑1 (MQ=255)                     aaTTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGc                                                                                                    >  1:184426/1‑136 (MQ=255)                     aaTTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGc                                                                                                    <  2:184426/136‑1 (MQ=255)                     aaTTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGt                                                                                                 >  4:83207/1‑139 (MQ=255)                     aaTTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTATGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCAc                                                                                                                  >  4:204658/1‑122 (MQ=255)                       ttGATTCTGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACATGCCATTTTGATTCGTGAACAACAGGGAACTACGGCGATTGGCAGGATTATCGCGATTCCGCACGGAAAGTCTGAGGCGGt                                                                                                 >  8:173387/1‑137 (MQ=255)                          aTTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACCACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCCgg                                                                                             >  8:232722/1‑138 (MQ=255)                                           aaGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCttt                                                                           <  6:127321/139‑1 (MQ=255)                                           aaGAGGTGCGCGGCTTTACGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCttt                                                                           <  4:268951/139‑1 (MQ=255)                                                gTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATACCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGAt                                                                      >  7:122933/1‑139 (MQ=255)                                                   cgcTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCa                                                                    >  2:16809/1‑138 (MQ=255)                                                    tcggctTTCAGTGAGCGGGGTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCAGGAATATCGCGATTCCACACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCaaa                                                                  <  2:416021/136‑1 (MQ=255)                                                    gctgctTTCAGAGAGCGGGTTTAAACAAGCCATTTTGACTCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCaaa                                                                  <  1:377939/139‑1 (MQ=255)                                                        ctTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATGCAACGAt                                                              >  2:203789/1‑139 (MQ=255)                                                        ctTTCAGAGAGCGGGTTTAAACAAGCCATTCTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGAt                                                              >  7:48485/1‑139 (MQ=255)                                                          ttCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGAt                                                              >  2:284107/1‑137 (MQ=255)                                                                 agCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGtt                                                      >  1:10842/1‑138 (MQ=255)                                                                  gCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGTGAACAACTGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGATCAGAGTTTTGCATTTGGGATCAAACGATCAGGCGGTgt                                                    >  2:428122/1‑138 (MQ=255)                                                                  gCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    >  5:436281/1‑139 (MQ=255)                                                                  gCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    >  2:73477/1‑139 (MQ=255)                                                                  gCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    >  1:335268/1‑139 (MQ=255)                                                                  gCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    >  7:79675/1‑139 (MQ=255)                                                                   cGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGAt                                                   >  5:222858/1‑139 (MQ=255)                                                                       tttAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               <  6:176871/139‑1 (MQ=255)                                                                       tttAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               <  6:222858/139‑1 (MQ=255)                                                                       tttAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATACCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               <  8:122933/139‑1 (MQ=255)                                                                        ttAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAAAATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               <  7:379474/138‑1 (MQ=255)                                                                         tAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGaa                                              <  1:284107/138‑1 (MQ=255)                                                                            aCAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGc                                          <  3:25774/139‑1 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACATGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGTGAGCCGAGTGTTGCCTTTGGGATCAAACGATCATGCGTTGAATGGAATAGCCtg                                       >  4:377741/1‑138 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACgg                                                                                                                >  3:287356/1‑65 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACgg                                                                                                                <  4:287356/65‑1 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAACCGATCAGGCGTGGATTGGAATAGCCtg                                       >  4:376470/1‑138 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtg                                       >  8:1241/1‑138 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtg                                       >  2:279928/1‑138 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtg                                       >  2:384571/1‑138 (MQ=255)                                                                                gCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtg                                       >  2:56849/1‑138 (MQ=255)                                                                                 ccATTCTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     <  8:48485/139‑1 (MQ=255)                                                                                     tttGAATCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGACGAGTGTTGCCTTTGGGATCAAACGATCAGGCCTTGATTGGAATAGCCtggatgga                                 >  7:223060/1‑139 (MQ=255)                                                                                           tCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGcc                                                                                        >  7:50251/1‑78 (MQ=255)                                                                                           tCGTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGcc                                                                                        <  8:50251/78‑1 (MQ=255)                                                                                            cgggAACAACAGGGAACAACGGCGAGTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAg                          <  8:123151/136‑1 (MQ=255)                                                                                             gTGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAg                          <  2:335268/138‑1 (MQ=255)                                                                                              tGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGc                         >  7:317790/1‑138 (MQ=255)                                                                                              tGAACAACAGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCt                        >  8:82381/1‑139 (MQ=255)                                                                                                   aacaGGGAACAACGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAAt                    >  4:123311/1‑138 (MQ=255)                                                                                                          aacaacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCtt             >  8:51560/1‑138 (MQ=255)                                                                                                          aacaacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCtt             >  8:230159/1‑138 (MQ=255)                                                                                                          aacaacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCtt             >  4:423549/1‑138 (MQ=255)                                                                                                          aacaacGGCGATTGGCATGAATATCGCGATTACGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATAAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGAGCAGCAGCTAAATTAATCtt             >  2:278851/1‑138 (MQ=255)                                                                                                            caacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTAt          <  6:436281/139‑1 (MQ=255)                                                                                                            caacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTAt          <  5:52667/139‑1 (MQ=255)                                                                                                             aacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTa                  <  7:212102/130‑1 (MQ=255)                                                                                                             aacGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTa                  >  8:212102/1‑130 (MQ=255)                                                                                                              acGGCCATTGGCATGAATATCGCGATTCCGCACGGCAAGTCGGAGGCGGTCAGGGAGCCGAGGGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGa        <  2:45432/139‑1 (MQ=255)                                                                                                               cGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGaa                                              <  6:265053/100‑1 (MQ=255)                                                                                                               cGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGaa                                              >  5:265053/1‑100 (MQ=255)                                                                                                               cGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     >  7:118026/1‑109 (MQ=255)                                                                                                               cGGCGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     <  8:118026/109‑1 (MQ=255)                                                                                                                  cGATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGAACAAACGATCAGGCGTTGATTGGAATAGCCTTGATGGATCAGAAGCTAAAATATTCTTTATGATTGc    >  6:45853/1‑139 (MQ=255)                                                                                                                   gATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     >  5:332270/1‑105 (MQ=255)                                                                                                                   gATTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     <  6:332270/105‑1 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    <  2:281714/89‑1 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGa                                                    >  1:281714/1‑89 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               <  4:184597/94‑1 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGa                                               >  3:184597/1‑94 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCtgga                                     <  5:201848/104‑1 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGATTGCTg  >  7:138424/1‑139 (MQ=255)                                                                                                                    aTTGGCATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGATTGCTg  >  2:10862/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                       GAGTGATGACATCATAGATGAATTGATTCAGAAATTGTCACGAAGAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGACATGAATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGATTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGATTGCTG  >  CP006881/1237315‑1237568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑