Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,176,333	G→A	V146V (GTC→GTT)	U712_10885 ←	putative deacetylase ypjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,176,333	0	G	A	100.0%	193.9 / NA	61	V146V (GTC→GTT)	U712_10885	putative deacetylase ypjG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/27);  total (34/27)

GAGTTGAAACTGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCATTGAGGCTTTGTTTTTTGGCCTCTATTGTATCCGAGATATCAATGACAAAATCAGGCTGATGAAAACCATTTATCATATAGTAGTAGACCTTGTTTACTTTATGCGCGGGAAGGCTTTTTTCGTCTTTATATTTGTGGATTCCTGCGGAAAAGATTGCTTCTTCCACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAA  >  CP006881/2176200‑2176467                                                                                                                                      |                                                                                                                                       gagTAGAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCtt                                                                                                                                   >  7:83523/1‑139 (MQ=255)  agTAGAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  >  8:208004/1‑139 (MQ=255)  agTAGAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  >  6:223614/1‑139 (MQ=255)  agTAGAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  <  4:167335/139‑1 (MQ=255)   gtagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTgc                                                                                                                                 <  6:367604/136‑2 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTgct                                                                                                                                >  4:267576/3‑137 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTgct                                                                                                                                >  3:309880/3‑137 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  >  4:319320/3‑137 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  <  4:318344/135‑1 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  <  3:319320/135‑1 (MQ=255)    tagAAACGGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  >  3:318344/3‑137 (MQ=255)         acggAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAAACTTGCTGACtt                                                                                                                           >  6:68957/4‑139 (MQ=255)            gAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTAt                                                                                                                        >  3:45777/1‑139 (MQ=255)                    tGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCcgg                                                                                                                 >  8:331478/1‑138 (MQ=255)                    tGACGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCATTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCcgg                                                                                                                 <  8:48913/138‑1 (MQ=255)                       cGGGATAAACTGGCTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAgg                                                                                                             <  7:390905/139‑1 (MQ=255)                                    cTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  >  5:423981/1‑105 (MQ=255)                                    cTTTTGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTg                                                                                                                                  <  6:423981/105‑1 (MQ=255)                                       ttGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTta                                                                                              <  4:309880/138‑1 (MQ=255)                                        tGTAGGCGTTGAGGCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTtata                                                                                            >  1:102253/1‑139 (MQ=255)                                                     gCTTTGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTccg                                                                               <  4:405587/139‑2 (MQ=255)                                                        ttGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTcaggcg                                                                            >  1:65417/1‑134 (MQ=255)                                                        ttGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCg                                                                            >  7:259308/1‑139 (MQ=255)                                                         tGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCcggcgg                                                                           >  5:341028/1‑139 (MQ=255)                                                         tGTTTCTTTGCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCg                                                                            >  8:348236/1‑138 (MQ=255)                                                               tttgCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGaa                                                                         >  2:113458/4‑135 (MQ=255)                                                                ttgCCTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGaa                                                                         >  6:395483/3‑134 (MQ=255)                                                                   ccTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGAtcg                                                                  <  5:395483/138‑3 (MQ=255)                                                                   ccTCTATTGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGAtcg                                                                  <  6:341028/138‑3 (MQ=255)                                                                         ttGTATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGcttct                                                             <  3:404523/137‑1 (MQ=255)                                                                            tATCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        <  6:119179/139‑1 (MQ=255)                                                                             aTCCGAGATATCAATAACAAAATCCGGCTGATGAAATCCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        <  2:102253/138‑1 (MQ=255)                                                                              tCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATTGATTACGGCGGAAAAGATCGCTTCTTCAAc                                                        >  2:415874/1‑137 (MQ=255)                                                                              tCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        >  6:45490/1‑137 (MQ=255)                                                                              tCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        >  1:39699/1‑137 (MQ=255)                                                                              tCCGAGATATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        >  3:153767/1‑137 (MQ=255)                                                                                 gagaTATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTg                                                   >  3:69062/1‑139 (MQ=255)                                                                                   gaTATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        <  4:424675/132‑1 (MQ=255)                                                                                   gaTATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCAc                                                        >  3:424675/1‑132 (MQ=255)                                                                                   gaTATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTg                                                   >  2:267691/1‑137 (MQ=255)                                                                                   gaTATCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTg                                                   >  7:361056/1‑137 (MQ=255)                                                                                     tatCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAg                                                <  4:245309/138‑1 (MQ=255)                                                                                     tatCAATAACAAAATCCGGCTGATGAAAACCAATTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAg                                                <  5:372265/138‑1 (MQ=255)                                                                                      atCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGcc                                              >  7:432603/1‑139 (MQ=255)                                                                                      atCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGcc                                              >  7:352891/1‑139 (MQ=255)                                                                                      atCAATAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGcc                                              >  4:149433/1‑139 (MQ=255)                                                                                         aaTAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCa                                           <  8:432603/139‑1 (MQ=255)                                                                                         aaTAACAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCa                                           <  1:113458/139‑1 (MQ=255)                                                                                          ataaCAAAATCCGGCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCAt                                          >  3:252330/4‑139 (MQ=255)                                                                                                   tccggCTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGAt                                 <  3:267576/136‑1 (MQ=255)                                                                                                        cTGATGAAAACCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGa                            >  3:204945/1‑139 (MQ=255)                                                                                                              aaaaCCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCg                       <  5:139430/138‑1 (MQ=255)                                                                                                               aaaCCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGAt                     <  1:415874/139‑1 (MQ=255)                                                                                                               aaaCCATTTATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGAt                     <  5:45490/139‑1 (MQ=255)                                                                                                               aaaCCATTTATCATATAATAGTAAACCTTGCTGACTTTAAGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGAt                     <  1:378231/139‑1 (MQ=255)                                                                                                                     tttATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGct                                                                <  2:330430/90‑1 (MQ=255)                                                                                                                     tttATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGct                                                                >  1:330430/1‑90 (MQ=255)                                                                                                                     tttATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCtttt               >  2:379392/1‑139 (MQ=255)                                                                                                                     tttATCATATAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCtttt               >  7:81203/1‑139 (MQ=255)                                                                                                                           atatAATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATAc          <  3:149433/138‑1 (MQ=255)                                                                                                                            tataATAGTAAACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACgg        <  4:252330/139‑1 (MQ=255)                                                                                                                                 tagtaaACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATa    >  3:300013/1‑138 (MQ=255)                                                                                                                                   gtaaACCTTGCTGACTTTATGCGCCGGAAGGCTTTTTTCGTCTTTATATTTATGGATTCCGGCGGAAAAGATCGCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATaaa  <  4:153767/138‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GAGTTGAAACTGAATCCTTTGACGGGATAAACTGGCTTTTGTAGGCATTGAGGCTTTGTTTTTTGGCCTCTATTGTATCCGAGATATCAATGACAAAATCAGGCTGATGAAAACCATTTATCATATAGTAGTAGACCTTGTTTACTTTATGCGCGGGAAGGCTTTTTTCGTCTTTATATTTGTGGATTCCTGCGGAAAAGATTGCTTCTTCCACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAA  >  CP006881/2176200‑2176467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑