| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 1,251,617 | A→G | K15E (AAA→GAA) | U712_06305 → | Uncharacterized protein yjgD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,251,617 | 0 | A | G | 98.4% | 191.2 / ‑4.8 | 63 | K15E (AAA→GAA) | U712_06305 | Uncharacterized protein yjgD |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (29/32); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
AATCGGTGTGCAGGTTCATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAAAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCGGGGTCCTGCCGTTGCTCCGCGGCCTTT > CP006881/1251492‑1251752 | aaTCGGTGGTCAGGTTCATAAGCAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCGAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCg < 6:63/139‑1 (MQ=255) gtgtGCAGGTTCATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGc > 3:302043/1‑139 (MQ=255) gTTCATAAGAAATGGGCTCTGAACGATTATATTTTCCCTGGTGACGCAGTGAAATGGGGGAAGTGCCATAATGGATAAAGCGATTAAACGAATCCAAAAACTCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTg < 3:234432/139‑1 (MQ=255) gTTCATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTg > 6:304296/1‑139 (MQ=255) gTTCATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTg > 7:155037/1‑139 (MQ=255) cATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCgg > 7:6840/1‑139 (MQ=255) cATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCgg > 3:180072/1‑139 (MQ=255) tAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCggg > 1:130768/1‑138 (MQ=255) tAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCggg > 2:166949/1‑138 (MQ=255) tAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAACCGTGATTTGCggg > 6:120662/1‑138 (MQ=255) aaTGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAAtt > 7:208510/1‑138 (MQ=255) aTGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGAGAAAAGGGGGATGGGCCATAATGGCTTAAGCGATTTAACGTATCCATAAAATCGAGGTTACAGAAGAGGATCAGCGAAAGCGTGATATGGGGGTAATTg > 7:156591/1‑138 (MQ=255) ggCGCGGAAGTATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGa < 5:116787/139‑1 (MQ=255) atatTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATACAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGAc < 5:304296/138‑1 (MQ=255) cccTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGaa < 2:183792/116‑1 (MQ=255) cccTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGaa > 1:183792/1‑116 (MQ=255) aCGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATtc > 2:363960/1‑139 (MQ=255) cTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACCGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCg < 5:278008/139‑1 (MQ=255) cTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCg < 1:394660/139‑1 (MQ=255) tgAATAGGGGGTTGTGCCATAATGGCTAAAGCTATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGaaa < 3:337946/139‑1 (MQ=255) gAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGaaa < 5:56310/138‑1 (MQ=255) gAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAAc < 1:363960/139‑1 (MQ=255) aaaaGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAAc < 5:120662/138‑1 (MQ=255) aaaGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACa < 1:166949/138‑1 (MQ=255) gggggCTGGGCCATACTGGCGAACGCGAGTAAACCAATCCAAAAACGCGAGGGAACAGAAGAGGCTCCGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTACTTGCCCACAAAGAAGCGATTCTCGAAACATTGc < 5:10978/139‑1 (MQ=255) gggggATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGAGCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGc < 7:436970/139‑1 (MQ=255) gggggATGGGCCATAATGGCTAAAGCGAATAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGc < 1:160207/139‑1 (MQ=255) ggggATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCa < 4:180072/139‑1 (MQ=255) gATGGGCCATAATGGCTAAAGCGATTAACCGAATCCAAAAAATCGAGGTAACAGATGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCatat > 5:361989/1‑139 (MQ=255) gATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCTATTCTCGAAACATTGCatat > 6:144248/1‑139 (MQ=255) gATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCatat > 8:443772/1‑139 (MQ=255) aTGGGCAATACTGGCTAAAGCTATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGCTCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATg < 1:346685/139‑1 (MQ=255) tGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAAAAGAGGATCAGCGAAAGCGTGATTTGC‑GGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCt > 8:113737/1‑139 (MQ=255) ggCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAATATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTg < 2:130768/139‑1 (MQ=255) ggCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGAGCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTg < 3:59997/139‑1 (MQ=255) ccctAAGGGCTTAAGCGATCACACGAATCCAAAAAATCGCGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTggg < 5:6816/136‑1 (MQ=255) ccATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTggg < 8:155037/139‑1 (MQ=255) caaTAATGGCTAAATCCATAAAACCCATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCTTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTggg < 1:415883/137‑1 (MQ=255) cTAAAGCTATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGa < 5:61416/112‑1 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGaa < 5:290405/77‑1 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGaa > 6:290405/1‑77 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGa > 6:61416/1‑112 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTggg < 1:239819/130‑1 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTggg > 2:239819/1‑130 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGaa > 8:152881/1‑139 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGaa > 3:131023/1‑139 (MQ=255) cTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGaa > 5:411100/1‑139 (MQ=255) cTAAAGCGAATAAACGAATCCAAAAAATCGCGGTAACACAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGACGATCCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGaa < 4:302043/139‑1 (MQ=255) ataaaaCGAAACAAAAAAATCTACGTAACAGAAGAGGAGCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGcgcg < 2:161842/136‑1 (MQ=255) aTTATACGAATCCAAAAAATCGACGAAACAGAAGAGGATGAGCGAAAGCGAGTTTTGCGGGAAATTGAAGATGCTCTAAATCACCACAAAGAAGCGAAACACGAAACATTCCATATGCTGGGCCAAATGAACGAGcgcg < 8:156591/139‑1 (MQ=255) ttAAACGAATCCAACAAATCGAGGTAACAGAAGAGGATCTGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCGAATTGACCACAATGAAGCGATTCTCGAAACATTTCATATGCTGGGCCTTATGAACGAGagcgc > 8:176296/1‑138 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGaaa < 7:445392/105‑1 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGaaa > 8:445392/1‑105 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGcgcg < 8:430972/138‑1 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGcgcg > 7:430972/1‑138 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCgg > 4:189592/1‑139 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCgg > 1:172956/1‑139 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCgg > 1:267182/1‑139 (MQ=255) ttAAACGAATCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAAGTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCgg > 8:36571/1‑139 (MQ=255) aTCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAAc < 7:83086/98‑1 (MQ=255) aTCCAAAAAATCGAGGTAACAGAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAAc > 8:83086/1‑98 (MQ=255) gAGGTAACAGAAGACGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCGGGGTCCTGCCGTTGCTCcg < 8:208510/137‑1 (MQ=255) aCAGAAGAGGATCAGCGAAAGCGTGATTGGCGGGAAATTGAAGATGCTCTAAATGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCGGGGTCCTGCCGTTGCTCCGCGGCCttt < 5:329198/139‑1 (MQ=255) | AATCGGTGTGCAGGTTCATAAGAAATGGGCGCGGAAGGATTATATTTTCCCTGGTGACGCTGTGAAAAGGGGGATGGGCCATAATGGCTAAAGCGATTAAACGAATCCAAAAAATCGAGGTAACAAAAGAGGATCAGCGAAAGCGTGATTTGCGGGAAATTGAAGATGCTCTAATTGACCACAAAGAAGCGATTCTCGAAACATTGCATATGCTGGGCCATATGAACGAGCGCGGGGTCCTGCCGTTGCTCCGCGGCCTTT > CP006881/1251492‑1251752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |