Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,176,537	A→G	G78G (GGT→GGC)	U712_10885 ←	putative deacetylase ypjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,176,537	0	A	G	100.0%	184.6 / NA	57	G78G (GGT→GGC)	U712_10885	putative deacetylase ypjG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/32);  total (25/32)

TTGCTTCTTCCACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATAT  >  CP006881/2176401‑2176665                                                                                                                                         |                                                                                                                                 tcgCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGcc                                                                                                                                <  3:425735/137‑1 (MQ=255)  cgCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGccg                                                                                                                               <  7:123298/138‑2 (MQ=255)   gCTTCTTCCACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGccg                                                                                                                               <  4:204945/138‑2 (MQ=255)           cACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGa                                                                                                                      <  5:389757/139‑1 (MQ=255)           cACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGa                                                                                                                      <  5:240150/139‑1 (MQ=255)                gTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgt                                                                                                                 <  3:217425/139‑1 (MQ=255)                 tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgtt                                                                                                                >  2:44077/1‑138 (MQ=255)                 tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgtt                                                                                                                >  3:284019/1‑138 (MQ=255)                 tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgt                                                                                                                 >  4:198859/1‑138 (MQ=255)                   cAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAg                                                                                                              >  3:438001/1‑139 (MQ=255)                     gCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGATTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  1:379392/139‑1 (MQ=255)                     gCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  7:208004/139‑1 (MQ=255)                       cGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGa                                                                                                          <  4:300013/139‑1 (MQ=255)                       cGCATTGACGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGAGTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGa                                                                                                          <  5:68957/139‑1 (MQ=255)                         cATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAAt                                                                                                        <  4:45777/139‑1 (MQ=255)                           ttGCCGTGATCCGGATGGCGATCCATATTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTATGACGCGGTCTGGAAGCGTTATCTGAATTc                                                                                                      >  8:7045/1‑139 (MQ=255)                                   aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                              >  3:58078/1‑139 (MQ=255)                                   aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                              >  7:95690/1‑139 (MQ=255)                                      cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcg                                                                                           <  1:65726/139‑2 (MQ=255)                                      cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCGTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTg                                                                                             <  8:352891/137‑1 (MQ=255)                                       ggATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcgc                                                                                          <  4:418466/139‑3 (MQ=255)                                        gATGGCGCTCCTTTTTATACGGCATAAAATCCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGcc                                                                                         >  3:115614/1‑139 (MQ=255)                                        gATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGATGAATTCTTTTATCTGCGcc                                                                                         >  5:177356/1‑139 (MQ=255)                                         aTGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCt                                                                                        <  2:39699/139‑1 (MQ=255)                                             cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCt                                                                                                     >  3:128296/1‑122 (MQ=255)                                             cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCt                                                                                                     <  4:128296/122‑1 (MQ=255)                                             cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATa                                                                                    >  1:166879/1‑139 (MQ=255)                                                     tttATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGgc                                                                             <  8:361056/138‑1 (MQ=255)                                                            ggCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAg                                                                     >  5:137641/1‑139 (MQ=255)                                                              cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCCgct                                                                   >  4:312692/1‑139 (MQ=255)                                                              cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                   >  6:391775/1‑139 (MQ=255)                                                              cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                   >  3:362562/1‑139 (MQ=255)                                                                  aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                               <  1:44077/133‑1 (MQ=255)                                                                  aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                               <  1:381133/133‑1 (MQ=255)                                                                      ccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttc                                                           <  4:284019/137‑1 (MQ=255)                                                                        gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGccg                                                                                                                               <  4:376298/69‑2 (MQ=255)                                                                        gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGccg                                                                                                                               >  3:376298/1‑68 (MQ=255)                                                                        gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  2:245467/88‑1 (MQ=255)                                                                        gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            >  1:245467/1‑88 (MQ=255)                                                                        gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttctt                                                         >  6:57048/1‑139 (MQ=255)                                                                                   acAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTg                                              >  3:218723/2‑139 (MQ=255)                                                                                        ttCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                         <  8:95690/139‑1 (MQ=255)                                                                                        tgatgctgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCTGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                         <  4:58078/133‑1 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTTGCTGATCACTCATTATTAGGCCGCGGTCTGGAATCGGTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                       <  1:252107/136‑3 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    >  6:85466/1‑139 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    >  7:12557/1‑139 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    <  3:387517/139‑1 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    <  2:166879/139‑1 (MQ=255)                                                                                             atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                       <  4:115614/136‑3 (MQ=255)                                                                                                  agtGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtaccgttag                                 >  4:222143/3‑135 (MQ=255)                                                                                                             accGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGc                    <  7:260912/138‑1 (MQ=255)                                                                                                                  aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg               >  7:136923/1‑137 (MQ=255)                                                                                                                  aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg               >  1:241206/1‑137 (MQ=255)                                                                                                                          aTCACTCATTATTAGGCCTCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTTCGAATAATATGCGGGCTTCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCTCTTCTGTCAAATCg       <  7:7045/139‑1 (MQ=255)                                                                                                                          aTCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCg       <  4:69062/139‑1 (MQ=255)                                                                                                                             aCTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCa     <  6:137641/138‑1 (MQ=255)                                                                                                                                cattattaGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCatat  >  5:163490/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                 TTGCTTCTTCCACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATAT  >  CP006881/2176401‑2176665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑