Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,176,540 A→G R77R (CGT→CGC)  U712_10885 ← putative deacetylase ypjG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,176,5400AG98.1% 164.9 / NA 52R77R (CGT→CGC) U712_10885putative deacetylase ypjG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (24/27);  minor base T (0/1);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATAT  >  CP006881/2176411‑2176665
                                                                                                                                 |                                                                                                                             
cACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGa                                                                                                                      <  5:389757/139‑1 (MQ=255)
cACCAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGa                                                                                                                      <  5:240150/139‑1 (MQ=255)
     gTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgt                                                                                                                 <  3:217425/139‑1 (MQ=255)
      tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgtt                                                                                                                >  3:284019/1‑138 (MQ=255)
      tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgtt                                                                                                                >  2:44077/1‑138 (MQ=255)
      tGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAgcgt                                                                                                                 >  4:198859/1‑138 (MQ=255)
        cAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAg                                                                                                              >  3:438001/1‑139 (MQ=255)
          gCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGATTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  1:379392/139‑1 (MQ=255)
          gCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  7:208004/139‑1 (MQ=255)
            cGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGa                                                                                                          <  4:300013/139‑1 (MQ=255)
            cGCATTGACGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGAGTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGa                                                                                                          <  5:68957/139‑1 (MQ=255)
              cATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAAt                                                                                                        <  4:45777/139‑1 (MQ=255)
                ttGCCGTGATCCGGATGGCGATCCATATTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTATGACGCGGTCTGGAAGCGTTATCTGAATTc                                                                                                      >  8:7045/1‑139 (MQ=255)
                        aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                              >  7:95690/1‑139 (MQ=255)
                        aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                              >  3:58078/1‑139 (MQ=255)
                           cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcg                                                                                           <  1:65726/139‑2 (MQ=255)
                           cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCGTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTg                                                                                             <  8:352891/137‑1 (MQ=255)
                            ggATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcgc                                                                                          <  4:418466/139‑3 (MQ=255)
                             gATGGCGCTCCTTTTTATACGGCATAAAATCCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGcc                                                                                         >  3:115614/1‑139 (MQ=255)
                             gATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGATGAATTCTTTTATCTGCGcc                                                                                         >  5:177356/1‑139 (MQ=255)
                              aTGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCt                                                                                        <  2:39699/139‑1 (MQ=255)
                                  cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCt                                                                                                     >  3:128296/1‑122 (MQ=255)
                                  cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCt                                                                                                     <  4:128296/122‑1 (MQ=255)
                                  cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATa                                                                                    >  1:166879/1‑139 (MQ=255)
                                          tttATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGgc                                                                             <  8:361056/138‑1 (MQ=255)
                                                 ggCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAg                                                                     >  5:137641/1‑139 (MQ=255)
                                                   cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCCgct                                                                   >  4:312692/1‑139 (MQ=255)
                                                   cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                   >  3:362562/1‑139 (MQ=255)
                                                   cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                   >  6:391775/1‑139 (MQ=255)
                                                       aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                               <  1:44077/133‑1 (MQ=255)
                                                       aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                               <  1:381133/133‑1 (MQ=255)
                                                           ccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttc                                                           <  4:284019/137‑1 (MQ=255)
                                                             gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            <  2:245467/88‑1 (MQ=255)
                                                             gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCt                                                                                                            >  1:245467/1‑88 (MQ=255)
                                                             gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttctt                                                         >  6:57048/1‑139 (MQ=255)
                                                                        acAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTg                                              >  3:218723/2‑139 (MQ=255)
                                                                             ttCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                         <  8:95690/139‑1 (MQ=255)
                                                                             tgatgctgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCTGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                         <  4:58078/133‑1 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTTGCTGATCACTCATTATTAGGCCGCGGTCTGGAATCGGTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                       <  1:252107/136‑3 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    >  6:85466/1‑139 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    >  7:12557/1‑139 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    <  3:387517/139‑1 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                    <  2:166879/139‑1 (MQ=255)
                                                                                  atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                       <  4:115614/136‑3 (MQ=255)
                                                                                       agtGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtaccgttag                                 >  4:222143/3‑135 (MQ=255)
                                                                                                  accGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGc                    <  7:260912/138‑1 (MQ=255)
                                                                                                       aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg               >  7:136923/1‑137 (MQ=255)
                                                                                                       aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg               >  1:241206/1‑137 (MQ=255)
                                                                                                               aTCACTCATTATTAGGCCTCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTTCGAATAATATGCGGGCTTCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCTCTTCTGTCAAATCg       <  7:7045/139‑1 (MQ=255)
                                                                                                               aTCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCg       <  4:69062/139‑1 (MQ=255)
                                                                                                                  aCTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCa     <  6:137641/138‑1 (MQ=255)
                                                                                                                     cattattaGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCatat  >  5:163490/1‑138 (MQ=255)
                                                                                                                                 |                                                                                                                             
CACTAGTGCAGCCGCATTGCCGTGATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATAT  >  CP006881/2176411‑2176665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑