Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,176,570	T→A	E67D (GAA→GAT)	U712_10885 ←	putative deacetylase ypjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,176,570	0	T	A	100.0%	190.2 / NA	62	E67D (GAA→GAT)	U712_10885	putative deacetylase ypjG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (36/26);  total (36/26)

ATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATATCATTAC‑TTTTTTTCCCTGTTTGACAAACTTCGCGAT  >  CP006881/2176435‑2176701                                                                                                                                        |                                                                                                                                     aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                                                                   >  3:58078/1‑139 (MQ=255) aTCCGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCt                                                                                                                                   >  7:95690/1‑139 (MQ=255)    cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcg                                                                                                                                <  1:65726/139‑2 (MQ=255)    cGGATGGCGATCCTTTTTATACGGCATAAAAACCGCGTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTg                                                                                                                                  <  8:352891/137‑1 (MQ=255)     ggATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGcgc                                                                                                                               <  4:418466/139‑3 (MQ=255)      gATGGCGCTCCTTTTTATACGGCATAAAATCCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGcc                                                                                                                              >  3:115614/1‑139 (MQ=255)      gATGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGATGAATTCTTTTATCTGCGcc                                                                                                                              >  5:177356/1‑139 (MQ=255)       aTGGCGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCt                                                                                                                             <  2:39699/139‑1 (MQ=255)           cGATCCTTTTTATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATa                                                                                                                         >  1:166879/1‑139 (MQ=255)                   tttATACGGCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGgc                                                                                                                  <  8:361056/138‑1 (MQ=255)                          ggCATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAg                                                                                                          >  5:137641/1‑139 (MQ=255)                            cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCCgct                                                                                                        >  4:312692/1‑139 (MQ=255)                            cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                                                        >  6:391775/1‑139 (MQ=255)                            cATAAAAACCGCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAgct                                                                                                        >  3:362562/1‑139 (MQ=255)                                aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                                                                    <  1:44077/133‑1 (MQ=255)                                aaaaccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGctt                                                                                                    <  1:381133/133‑1 (MQ=255)                                    ccgCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttc                                                                                                <  4:284019/137‑1 (MQ=255)                                      gCTTTTGGCCGACAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGcttcttctt                                                                                              >  6:57048/1‑139 (MQ=255)                                                 acAGATTCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTg                                                                                   >  3:218723/2‑139 (MQ=255)                                                      ttCTGATGACAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                                                              <  8:95690/139‑1 (MQ=255)                                                      tgatgctgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCTGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgt                                                                              <  4:58078/133‑1 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTTGCTGATCACTCATTATTAGGCCGCGGTCTGGAATCGGTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                                                            <  1:252107/136‑3 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                                                         >  6:85466/1‑139 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                                                         >  7:12557/1‑139 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                                                         <  3:387517/139‑1 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGaccgtaccgt                                                                         <  2:166879/139‑1 (MQ=255)                                                           atgaCAGTGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                                                            <  4:115614/136‑3 (MQ=255)                                                                agtGACAATTGACCGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtaccgttag                                                                      >  4:222143/3‑135 (MQ=255)                                                                           accGAATTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGc                                                         <  7:260912/138‑1 (MQ=255)                                                                                aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg                                                    >  1:241206/1‑137 (MQ=255)                                                                                aTTGCCTGATCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTctg                                                    >  7:136923/1‑137 (MQ=255)                                                                                        aTCACTCATTATTAGGCCTCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTTCGAATAATATGCGGGCTTCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCTCTTCTGTCAAATCg                                            <  7:7045/139‑1 (MQ=255)                                                                                        aTCACTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCg                                            <  4:69062/139‑1 (MQ=255)                                                                                           aCTCATTATTAGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCa                                          <  6:137641/138‑1 (MQ=255)                                                                                              cattattaGGCCGCGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCatat                                       >  5:163490/1‑138 (MQ=255)                                                                                                       gccgcGGTCTGTAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGAGGGCTGCTTCAGCTGCTTCTTCTTTACGCTAACTGACCGTACCGTTAGAAGAGAGGTCCGCTTCTTTCAAATAGCATATCATTAA‑tttt                            >  5:200603/5‑139 (MQ=255)                                                                                                         cgcGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtaccg                                                                          >  8:58264/3‑92 (MQ=255)                                                                                                         cgcGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtaccg                                                                          <  7:58264/90‑1 (MQ=255)                                                                                                         cgcGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑tttttt                          >  3:8776/3‑139 (MQ=255)                                                                                                         cgcGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑tttttt                          >  6:388184/3‑139 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                                                            >  1:208899/1‑86 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGAccgtac                                                                            <  2:208899/88‑3 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCa                                          >  5:88793/1‑122 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCa                                          <  6:88793/122‑1 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑tttttttt                        >  6:130293/1‑138 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑tttttttt                        >  7:205775/1‑138 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑ttttttt                         >  7:371764/1‑138 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCAAATCAATAC‑ttttttt                         >  4:208258/1‑138 (MQ=255)                                                                                                           cGGTCTGGAAGCGTTAGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTAAGCAAACTGCCAGTAACGTTAGAATAGATTTCCGCTTCTGTAAAATCGCATAAAATTAA‑ttttttt                         >  2:29485/1‑138 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTc                                                  <  6:141774/99‑1 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTc                                                  >  5:141774/3‑101 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTGAAATAGCATATCATTAC‑TTTTTTTTACTTTTTTACaaa           >  8:382488/3‑139 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACaaa           >  5:300232/3‑139 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACaaa           <  8:12557/137‑1 (MQ=255)                                                                                                                        ttaGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACaa            >  4:226173/3‑138 (MQ=255)                                                                                                                          aGCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCGTCATTACGCAAACTGACCGGACCGTTAGAAGAGAGTTACGCGTCTGTCAAATCGCATATCAATAA‑TTTTTTTTCCTGTTTGACAAACt         >  4:320740/1‑139 (MQ=255)                                                                                                                           gCTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACAAACtt        <  5:391775/139‑1 (MQ=255)                                                                                                                            cTGAATTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTTCCTGTTTGACAAACtt        <  5:166055/139‑1 (MQ=255)                                                                                                                               aaTTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACa             <  3:409352/130‑1 (MQ=255)                                                                                                                               aaTTCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACa             >  4:409352/1‑130 (MQ=255)                                                                                                                                 ttCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGCCCGTACCGTTAGAAGAGAGTTCCGCTTCTGTGAAATAGCATATAAATAG‑TTTTTTTTCCGGTTTGACAAACTTCGCTAt  >  2:3874/1‑139 (MQ=255)                                                                                                                                 ttCTTTTATCTGCGCCTAATATGCGGGCTGCTTCAGCTGCTTCTTCTTTACGCAAACTGACCGTACCGTTAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTAC‑TTTTTTTTCCTGTTTGACAAACTTCGCTAt  >  4:107212/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     ATCCGGATGGCGATCCTTTTTATACGGCATAAAGATTGCTTTGGGCCGGCAGGTTCTGATGACACTGACAATCGCCCGAATTGCCTGATCACTCATCATCAGACCACGGTCTGGAAGTGTCAGCTGAATTCTTTTTTCTGCACCTAATACACGGGCTGCTTCAGCTGCTTCTTCTTTACGTAAACTGACCGTTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATATCATTAC‑TTTTTTTCCCTGTTTGACAAACTTCGCGAT  >  CP006881/2176435‑2176701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑