Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,176,761 T→C T4A (ACT→GCT)  U712_10885 ← putative deacetylase ypjG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,176,7610TC98.5% 203.0 / ‑4.9 66T4A (ACT→GCT) U712_10885putative deacetylase ypjG
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (26/39);  total (27/39)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

TTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATATCATTACTTTTTTTCCCTGTTTGACAAACTTCGCGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAAC  >  CP006881/2176626‑2176889
                                                                                                                                       |                                                                                                                                
taccgttAGAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGcat                                                                                                                                >  3:194320/3‑138 (MQ=255)
  ccgttAGAAGAGAGTTGCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCAGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGcatta                                                                                                                              >  2:310512/1‑138 (MQ=255)
      tagAAGAGAGTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAt                                                                                                                         >  6:119170/3‑139 (MQ=255)
      tagAAGAGAGTTCCGCTTCAGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAt                                                                                                                         >  4:27629/3‑139 (MQ=255)
          agagagTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCgg                                                                                                                     <  3:198859/139‑1 (MQ=255)
          agagagTGCCGCTGCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCgg                                                                                                                     <  3:303345/139‑1 (MQ=255)
              agTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGctct                                                                                                                 <  1:326653/139‑1 (MQ=255)
              agTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGctct                                                                                                                 >  3:378080/1‑139 (MQ=255)
              agTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGctct                                                                                                                 >  6:233484/1‑139 (MQ=255)
               gTTCCGCTTCTGTCAAATCGCATATCATTTCTTTTTTTTCCTGTTTGACAAACTAAGCTATTGTGCCGCCCATTCCGATCTCGACATCAGCACTGTGGGCGCCAAAAGCAATAACGTCAGCATTATACATTCGGCtctt                                                                                                                >  3:119152/1‑139 (MQ=255)
               gTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCtctt                                                                                                                >  6:143864/1‑139 (MQ=255)
               gTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCtctt                                                                                                                >  5:162397/1‑139 (MQ=255)
               gTTCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCtctt                                                                                                                <  2:10430/139‑1 (MQ=255)
                ttCCGCTTCTGTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCtctt                                                                                                                <  2:229759/138‑1 (MQ=255)
                          gTCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGATATTGTGCCGCCCATGCCGATCTCGCAATCATCACTGTGGGCGCGAAAAGCAAGAACGTCAGCATTATACATTCTGCTGTTCTCCCCgaag                                                                                                      >  8:120932/1‑138 (MQ=255)
                           tCAAATCGCATATCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCgaaga                                                                                                     <  6:300232/138‑1 (MQ=255)
                                     tatCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGaa                                                                                          >  7:379454/1‑139 (MQ=255)
                                             cTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGaaaa                                                                                  <  4:8776/139‑1 (MQ=255)
                                             cTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGGCACGGAATTCGaaaa                                                                                  >  8:123033/1‑139 (MQ=255)
                                                ttttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAacac                                                                               >  5:5967/1‑139 (MQ=255)
                                                ttttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAaca                                                                                <  3:107212/138‑1 (MQ=255)
                                                  ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              <  3:27629/138‑1 (MQ=255)
                                                  ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              <  6:200603/138‑1 (MQ=255)
                                                  ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              <  5:233484/138‑1 (MQ=255)
                                                              aCAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGtgtg                                                                 <  6:162397/139‑1 (MQ=255)
                                                                aaaCTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCg                                                               <  8:121545/139‑1 (MQ=255)
                                                                        ctaTTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGaa                                                       >  5:413438/3‑139 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              <  1:362947/110‑1 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              >  2:362947/1‑110 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 >  5:334621/1‑139 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 >  1:40576/1‑139 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 >  3:136411/1‑139 (MQ=255)
                                                                              tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 <  3:208258/139‑1 (MQ=255)
                                                                                ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 >  7:443010/1‑137 (MQ=255)
                                                                                ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               <  6:413438/139‑1 (MQ=255)
                                                                                ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               <  7:132985/139‑1 (MQ=255)
                                                                                  gccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGt                                             <  4:218723/139‑1 (MQ=255)
                                                                                   cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGcatta                                                                                                                              <  3:234738/57‑1 (MQ=255)
                                                                                   cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGcatta                                                                                                                              >  4:234738/1‑57 (MQ=255)
                                                                                   cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               >  4:292732/1‑136 (MQ=255)
                                                                                   cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               <  3:292732/136‑1 (MQ=255)
                                                                                    ccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAc                                           >  5:35025/1‑139 (MQ=255)
                                                                                     cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              >  4:286174/1‑103 (MQ=255)
                                                                                     cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                              <  3:286174/103‑1 (MQ=255)
                                                                                     cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                          <  8:443010/139‑1 (MQ=255)
                                                                                        tCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCa                                        >  8:215664/1‑138 (MQ=255)
                                                                                            aTCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                   >  8:293418/1‑139 (MQ=255)
                                                                                              ctcGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATACGAAAACACCTTCATCAAGTGAGCGCTCAACAATATCCGCAGTACCCATATATGt                                 >  4:51649/1‑139 (MQ=255)
                                                                                                 gACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                   <  4:136411/134‑1 (MQ=255)
                                                                                                      catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                         <  7:185602/139‑1 (MQ=255)
                                                                                                      catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTAAACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                         <  1:310512/139‑1 (MQ=255)
                                                                                                       atcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                         <  6:163490/138‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGCACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  1:72617/139‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  1:49689/139‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  2:40576/139‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  6:5967/139‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  4:194320/139‑1 (MQ=255)
                                                                                                        tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAATCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  5:119170/139‑1 (MQ=255)
                                                                                                        gcaccgtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                       <  8:379695/133‑1 (MQ=255)
                                                                                                             gtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                  <  6:35025/138‑1 (MQ=255)
                                                                                                              tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                  <  8:379454/138‑1 (MQ=255)
                                                                                                              tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTACATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                 >  5:321515/1‑139 (MQ=255)
                                                                                                               gggCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaat                <  3:226173/139‑1 (MQ=255)
                                                                                                                        aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 >  7:257683/1‑97 (MQ=255)
                                                                                                                        aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                 <  8:257683/97‑1 (MQ=255)
                                                                                                                        aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                              >  5:29510/1‑100 (MQ=255)
                                                                                                                        aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                              <  6:29510/100‑1 (MQ=255)
                                                                                                                             aGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATaaa  <  1:3874/139‑2 (MQ=255)
                                                                                                                              gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               >  2:189513/1‑93 (MQ=255)
                                                                                                                              gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                               <  1:189513/93‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
TTCCGTTTGAAGAGAGTTCCGCTTCAGTCAAATCGCATATCATTACTTTTTTTCCCTGTTTGACAAACTTCGCGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAAC  >  CP006881/2176626‑2176889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑