Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,176,796 G→A R128R (CGC→CGT)  U712_10890 ← Methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,176,7960GA98.7% 230.0 / ‑4.9 77R128R (CGC→CGT) U712_10890Methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (36/40);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

TATCATTACTTTTTTTCCCTGTTTGACAAACTTCGCGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGC  >  CP006881/2176663‑2176927
                                                                                                                                     |                                                                                                                                   
tatCATTACTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGaa                                                                                                                                >  7:379454/1‑139 (MQ=255)
        cTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGaaaa                                                                                                                        <  4:8776/139‑1 (MQ=255)
        cTTTTTTTTCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGGCACGGAATTCGaaaa                                                                                                                        >  8:123033/1‑139 (MQ=255)
           ttttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAacac                                                                                                                     >  5:5967/1‑139 (MQ=255)
           ttttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAaca                                                                                                                      <  3:107212/138‑1 (MQ=255)
             ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    <  3:27629/138‑1 (MQ=255)
             ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    <  6:200603/138‑1 (MQ=255)
             ttttCCTGTTTGACAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    <  5:233484/138‑1 (MQ=255)
                         aCAAACTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGtgtg                                                                                                       <  6:162397/139‑1 (MQ=255)
                           aaaCTTCGCTATTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCg                                                                                                     <  8:121545/139‑1 (MQ=255)
                                   ctaTTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGaa                                                                                             >  5:413438/3‑139 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    >  2:362947/1‑110 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    <  1:362947/110‑1 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       >  5:334621/1‑139 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       >  1:40576/1‑139 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       >  3:136411/1‑139 (MQ=255)
                                         tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       <  3:208258/139‑1 (MQ=255)
                                           ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       >  7:443010/1‑137 (MQ=255)
                                           ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     <  6:413438/139‑1 (MQ=255)
                                           ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     <  7:132985/139‑1 (MQ=255)
                                             gccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGt                                                                                   <  4:218723/139‑1 (MQ=255)
                                              cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     <  3:292732/136‑1 (MQ=255)
                                              cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     >  4:292732/1‑136 (MQ=255)
                                               ccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAc                                                                                 >  5:35025/1‑139 (MQ=255)
                                                cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    <  3:286174/103‑1 (MQ=255)
                                                cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACAcc                                                                                                                    >  4:286174/1‑103 (MQ=255)
                                                cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                <  8:443010/139‑1 (MQ=255)
                                                   tCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCa                                                                              >  8:215664/1‑138 (MQ=255)
                                                       aTCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                         >  8:293418/1‑139 (MQ=255)
                                                         ctcGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATACGAAAACACCTTCATCAAGTGAGCGCTCAACAATATCCGCAGTACCCATATATGt                                                                       >  4:51649/1‑139 (MQ=255)
                                                            gACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                         <  4:136411/134‑1 (MQ=255)
                                                                 catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                               <  7:185602/139‑1 (MQ=255)
                                                                 catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTAAACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                               <  1:310512/139‑1 (MQ=255)
                                                                  atcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                               <  6:163490/138‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGCACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  1:72617/139‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  1:49689/139‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  4:194320/139‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  6:5967/139‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  2:40576/139‑1 (MQ=255)
                                                                   tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAATCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  5:119170/139‑1 (MQ=255)
                                                                   gcaccgtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                             <  8:379695/133‑1 (MQ=255)
                                                                        gtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                        <  6:35025/138‑1 (MQ=255)
                                                                         tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                        <  8:379454/138‑1 (MQ=255)
                                                                         tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTACATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                       >  5:321515/1‑139 (MQ=255)
                                                                          gggCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaat                                                      <  3:226173/139‑1 (MQ=255)
                                                                                   aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       <  8:257683/97‑1 (MQ=255)
                                                                                   aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                       >  7:257683/1‑97 (MQ=255)
                                                                                   aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                    >  5:29510/1‑100 (MQ=255)
                                                                                   aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                    <  6:29510/100‑1 (MQ=255)
                                                                                        aGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATaaa                                        <  1:3874/139‑2 (MQ=255)
                                                                                         gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     >  2:189513/1‑93 (MQ=255)
                                                                                         gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                     <  1:189513/93‑1 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCgc                                                                                                    >  5:88460/1‑63 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCgc                                                                                                    <  6:88460/64‑2 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGcc                            >  4:123478/1‑136 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCgag                         >  4:76469/1‑139 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                >  7:99299/1‑84 (MQ=255)
                                                                                                       taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                <  8:99299/84‑1 (MQ=255)
                                                                                                           ttCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTTGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACAt                     >  3:161787/1‑139 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                <  4:410205/79‑1 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                >  3:410205/1‑79 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                 <  7:232260/94‑1 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                 >  8:232260/1‑94 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGACTACACATCACATAAACGGATTAATGCCGAGACATc                    >  3:78781/1‑139 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                    >  2:124284/1‑139 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                    >  7:282049/1‑139 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                    >  5:50804/1‑139 (MQ=255)
                                                                                                            tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                    >  7:103499/1‑139 (MQ=255)
                                                                                                               gCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCgg                 >  7:182000/1‑139 (MQ=255)
                                                                                                                   ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                              >  6:394694/1‑74 (MQ=255)
                                                                                                                   ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                              <  5:394694/74‑1 (MQ=255)
                                                                                                                          cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCggat      >  4:12659/1‑137 (MQ=255)
                                                                                                                          cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCt      >  6:150343/1‑139 (MQ=255)
                                                                                                                          cGAAGAAGGTCACGGAAGTCGAAAACACCTACATCAAGTGTGCGCACAAGAATATCCCCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGGTCATGCGGCt      >  1:264053/1‑139 (MQ=255)
                                                                                                                              gaagGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTgcgc  <  3:76469/139‑1 (MQ=255)
                                                                                                                                  gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg           <  2:312642/126‑1 (MQ=255)
                                                                                                                                  gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg           >  1:312642/1‑126 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TATCATTACTTTTTTTCCCTGTTTGACAAACTTCGCGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGC  >  CP006881/2176663‑2176927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑