Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,176,829 G→C V117V (GTC→GTG)  U712_10890 ← Methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,176,8290GC98.7% 257.5 / NA 79V117V (GTC→GTG) U712_10890Methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (39/39);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAGATGACAAGATCGAG  >  CP006881/2176698‑2176963
                                                                                                                                   |                                                                                                                                      
ctaTTGTGCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGaa                                                                                                                                 >  5:413438/3‑139 (MQ=255)
      tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           <  3:208258/139‑1 (MQ=255)
      tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           >  3:136411/1‑139 (MQ=255)
      tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           >  1:40576/1‑139 (MQ=255)
      tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           >  5:334621/1‑139 (MQ=255)
        ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           >  7:443010/1‑137 (MQ=255)
        ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         <  6:413438/139‑1 (MQ=255)
        ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         <  7:132985/139‑1 (MQ=255)
          gccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGt                                                                                                                       <  4:218723/139‑1 (MQ=255)
           cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         <  3:292732/136‑1 (MQ=255)
           cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         >  4:292732/1‑136 (MQ=255)
            ccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAc                                                                                                                     >  5:35025/1‑139 (MQ=255)
             cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                    <  8:443010/139‑1 (MQ=255)
                tCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCa                                                                                                                  >  8:215664/1‑138 (MQ=255)
                    aTCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                                                             >  8:293418/1‑139 (MQ=255)
                      ctcGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATACGAAAACACCTTCATCAAGTGAGCGCTCAACAATATCCGCAGTACCCATATATGt                                                                                                           >  4:51649/1‑139 (MQ=255)
                         gACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                                                             <  4:136411/134‑1 (MQ=255)
                              catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                   <  7:185602/139‑1 (MQ=255)
                              catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTAAACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                   <  1:310512/139‑1 (MQ=255)
                               atcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                   <  6:163490/138‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGCACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  1:72617/139‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  6:5967/139‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  4:194320/139‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  1:49689/139‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  2:40576/139‑1 (MQ=255)
                                tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAATCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  5:119170/139‑1 (MQ=255)
                                gcaccgtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  8:379695/133‑1 (MQ=255)
                                     gtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                                                            <  6:35025/138‑1 (MQ=255)
                                      tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                                                            <  8:379454/138‑1 (MQ=255)
                                      tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTACATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                           >  5:321515/1‑139 (MQ=255)
                                       gggCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaat                                                                                          <  3:226173/139‑1 (MQ=255)
                                                aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           <  8:257683/97‑1 (MQ=255)
                                                aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                           >  7:257683/1‑97 (MQ=255)
                                                aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                                                        >  5:29510/1‑100 (MQ=255)
                                                aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                                                        <  6:29510/100‑1 (MQ=255)
                                                     aGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATaaa                                                                            <  1:3874/139‑2 (MQ=255)
                                                      gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         >  2:189513/1‑93 (MQ=255)
                                                      gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                         <  1:189513/93‑1 (MQ=255)
                                                                    taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGcc                                                                >  4:123478/1‑136 (MQ=255)
                                                                    taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCgag                                                             >  4:76469/1‑139 (MQ=255)
                                                                    taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                    >  7:99299/1‑84 (MQ=255)
                                                                    taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                    <  8:99299/84‑1 (MQ=255)
                                                                        ttCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTTGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACAt                                                         >  3:161787/1‑139 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                    <  4:410205/79‑1 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                    >  3:410205/1‑79 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                                                     >  8:232260/1‑94 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                                                     <  7:232260/94‑1 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGACTACACATCACATAAACGGATTAATGCCGAGACATc                                                        >  3:78781/1‑139 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                        >  7:282049/1‑139 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                        >  5:50804/1‑139 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                        >  2:124284/1‑139 (MQ=255)
                                                                         tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                        >  7:103499/1‑139 (MQ=255)
                                                                            gCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCgg                                                     >  7:182000/1‑139 (MQ=255)
                                                                                ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                                                                  <  5:394694/74‑1 (MQ=255)
                                                                                ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                                                                  >  6:394694/1‑74 (MQ=255)
                                                                                       cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCggat                                          >  4:12659/1‑137 (MQ=255)
                                                                                       cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCt                                          >  6:150343/1‑139 (MQ=255)
                                                                                       cGAAGAAGGTCACGGAAGTCGAAAACACCTACATCAAGTGTGCGCACAAGAATATCCCCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGGTCATGCGGCt                                          >  1:264053/1‑139 (MQ=255)
                                                                                           gaagGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTgcgc                                      <  3:76469/139‑1 (MQ=255)
                                                                                               gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg                                               >  1:312642/1‑126 (MQ=255)
                                                                                               gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg                                               <  2:312642/126‑1 (MQ=255)
                                                                                                   cgGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCg                              >  3:169847/1‑139 (MQ=255)
                                                                                                   cgGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCg                              >  3:163324/1‑139 (MQ=255)
                                                                                                          cGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 >  3:132883/1‑65 (MQ=255)
                                                                                                          cGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                 <  4:132883/65‑1 (MQ=255)
                                                                                                                cacCTTCATCAAGTGTGCGCACAATAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGACGAGACATCCGGTTCATGCTGCTGCGAGGACAGCGGGTCTCGCaaaa                  >  8:168424/1‑138 (MQ=255)
                                                                                                                cacCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCaaaa                  >  2:5165/1‑138 (MQ=255)
                                                                                                                   cTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAAt              >  2:147909/1‑139 (MQ=255)
                                                                                                                     tcatcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaa                                                                                                <  1:327992/55‑1 (MQ=255)
                                                                                                                     tcatcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaa                                                                                                >  2:327992/1‑55 (MQ=255)
                                                                                                                       atcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACa          <  3:123478/139‑1 (MQ=255)
                                                                                                                          aaGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGa       <  1:147909/139‑1 (MQ=255)
                                                                                                                           aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                           <  8:196197/54‑1 (MQ=255)
                                                                                                                           aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                           >  7:196197/1‑54 (MQ=255)
                                                                                                                           aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGAc           >  1:130886/1‑134 (MQ=255)
                                                                                                                           aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGAt      <  4:378080/139‑1 (MQ=255)
                                                                                                                            ctgtgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCTCGCAAAAAAATGACAAGATc     <  3:62124/138‑1 (MQ=255)
                                                                                                                               tgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAg  <  7:123033/139‑1 (MQ=255)
                                                                                                                               tgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAg  <  5:143864/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CGATTGTGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAGATGACAAGATCGAG  >  CP006881/2176698‑2176963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑