Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,176,838 C→T E114E (GAG→GAA)  U712_10890 ← Methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,176,8380CT97.5% 242.5 / ‑3.1 83E114E (GAG→GAA) U712_10890Methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (39/40);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

TGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAGATGACAAGATCGAGTGCATT  >  CP006881/2176704‑2176969
                                                                                                                                      |                                                                                                                                   
tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 >  3:136411/1‑139 (MQ=255)
tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 <  3:208258/139‑1 (MQ=255)
tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 >  1:40576/1‑139 (MQ=255)
tgCCGCCCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 >  5:334621/1‑139 (MQ=255)
  ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 >  7:443010/1‑137 (MQ=255)
  ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               <  7:132985/139‑1 (MQ=255)
  ccgccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               <  6:413438/139‑1 (MQ=255)
    gccCATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGt                                                                                                                             <  4:218723/139‑1 (MQ=255)
     cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               >  4:292732/1‑136 (MQ=255)
     cccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               <  3:292732/136‑1 (MQ=255)
      ccATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAc                                                                                                                           >  5:35025/1‑139 (MQ=255)
       cATTCCGATCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                          <  8:443010/139‑1 (MQ=255)
          tCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCa                                                                                                                        >  8:215664/1‑138 (MQ=255)
              aTCTCGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                                                                   >  8:293418/1‑139 (MQ=255)
                ctcGACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATACGAAAACACCTTCATCAAGTGAGCGCTCAACAATATCCGCAGTACCCATATATGt                                                                                                                 >  4:51649/1‑139 (MQ=255)
                   gACATCATCACTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATAttt                                                                                                                   <  4:136411/134‑1 (MQ=255)
                        catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                         <  7:185602/139‑1 (MQ=255)
                        catcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTAAACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                         <  1:310512/139‑1 (MQ=255)
                         atcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgagcg                                                                                                         <  6:163490/138‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGCACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  1:72617/139‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  4:194320/139‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  6:5967/139‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  2:40576/139‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  1:49689/139‑1 (MQ=255)
                          tcaCTGTGGGCGCCAAAAGCAAGAACGTCAGCATTATACAATCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  5:119170/139‑1 (MQ=255)
                          gcaccgtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  8:379695/133‑1 (MQ=255)
                               gtGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                                                                  <  6:35025/138‑1 (MQ=255)
                                tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatgga                                                                                                  <  8:379454/138‑1 (MQ=255)
                                tGGGCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTACATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                                 >  5:321515/1‑139 (MQ=255)
                                 gggCGCCAAAAGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaat                                                                                                <  3:226173/139‑1 (MQ=255)
                                          aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 >  7:257683/1‑97 (MQ=255)
                                          aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCg                                                                                                                                 <  8:257683/97‑1 (MQ=255)
                                          aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                                                              <  6:29510/100‑1 (MQ=255)
                                          aaGCAAGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAg                                                                                                                              >  5:29510/1‑100 (MQ=255)
                                               aGAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATaaa                                                                                  <  1:3874/139‑2 (MQ=255)
                                                gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               <  1:189513/93‑1 (MQ=255)
                                                gAACGTCAGCATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCa                                                                                                                               >  2:189513/1‑93 (MQ=255)
                                                              taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGcc                                                                      >  4:123478/1‑136 (MQ=255)
                                                              taCATTCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCgag                                                                   >  4:76469/1‑139 (MQ=255)
                                                              taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                          <  8:99299/84‑1 (MQ=255)
                                                              taCATTCGGCTCTTCTCCCCGAAGAAGGACACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                          >  7:99299/1‑84 (MQ=255)
                                                                  ttCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTTGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACAt                                                               >  3:161787/1‑139 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                          <  4:410205/79‑1 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTAcc                                                                                                                          >  3:410205/1‑79 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                                                           <  7:232260/94‑1 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTggcgag                                                                                                           >  8:232260/1‑94 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGACTACACATCACATAAACGGATTAATGCCGAGACATc                                                              >  3:78781/1‑139 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                              >  7:282049/1‑139 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                              >  5:50804/1‑139 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                              >  7:103499/1‑139 (MQ=255)
                                                                   tCGGCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATc                                                              >  2:124284/1‑139 (MQ=255)
                                                                      gCTCTTCTCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCgg                                                           >  7:182000/1‑139 (MQ=255)
                                                                          ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                                                                        <  5:394694/74‑1 (MQ=255)
                                                                          ttctCCCCGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCa                                                                                                                        >  6:394694/1‑74 (MQ=255)
                                                                                 cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCggat                                                >  4:12659/1‑137 (MQ=255)
                                                                                 cGAAGAAGGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCt                                                >  6:150343/1‑139 (MQ=255)
                                                                                 cGAAGAAGGTCACGGAAGTCGAAAACACCTACATCAAGTGTGCGCACAAGAATATCCCCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGGTCATGCGGCt                                                >  1:264053/1‑139 (MQ=255)
                                                                                     gaagGTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTgcgc                                            <  3:76469/139‑1 (MQ=255)
                                                                                         gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg                                                     <  2:312642/126‑1 (MQ=255)
                                                                                         gTCACGGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATg                                                     >  1:312642/1‑126 (MQ=255)
                                                                                             cgGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCg                                    >  3:169847/1‑139 (MQ=255)
                                                                                             cgGAATTCGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCg                                    >  3:163324/1‑139 (MQ=255)
                                                                                                    cGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       >  3:132883/1‑65 (MQ=255)
                                                                                                    cGAAAACACCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCgga                                                                                                       <  4:132883/65‑1 (MQ=255)
                                                                                                          cacCTTCATCAAGTGTGCGCACAATAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGACGAGACATCCGGTTCATGCTGCTGCGAGGACAGCGGGTCTCGCaaaa                        >  8:168424/1‑138 (MQ=255)
                                                                                                          cacCTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCaaaa                        >  2:5165/1‑138 (MQ=255)
                                                                                                             cTTCATCAAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAAt                    >  2:147909/1‑139 (MQ=255)
                                                                                                               tcatcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaa                                                                                                      <  1:327992/55‑1 (MQ=255)
                                                                                                               tcatcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaa                                                                                                      >  2:327992/1‑55 (MQ=255)
                                                                                                                 atcaAGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACa                <  3:123478/139‑1 (MQ=255)
                                                                                                                    aaGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGa             <  1:147909/139‑1 (MQ=255)
                                                                                                                     aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                                 >  7:196197/1‑54 (MQ=255)
                                                                                                                     aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCggaatggaa                                                                                                 <  8:196197/54‑1 (MQ=255)
                                                                                                                     aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGAc                 >  1:130886/1‑134 (MQ=255)
                                                                                                                     aGTGTGCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGAt            <  4:378080/139‑1 (MQ=255)
                                                                                                                      ctgtgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCTCGCAAAAAAATGACAAGATc           <  3:62124/138‑1 (MQ=255)
                                                                                                                         tgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAg        <  7:123033/139‑1 (MQ=255)
                                                                                                                         tgCGCACAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAg        <  5:143864/139‑1 (MQ=255)
                                                                                                                           cgcaCAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAGTg      >  8:415241/4‑139 (MQ=255)
                                                                                                                           cgcaCAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAACATCGAGTg      >  2:95044/4‑139 (MQ=255)
                                                                                                                           cgcaCAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGGCGCGCAAAAAAATGACAAGAACGAggg      >  1:159787/4‑137 (MQ=255)
                                                                                                                              aCAAGAATTTCCGCAGTACCCATATTTGTGGCGAGCGGAATGGAATACACATCACATAAACGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAAATGACAAGATCGAGTGCAt   <  6:321515/139‑1 (MQ=255)
                                                                                                                               cAAGAAGCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTGATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAGATGACAAGATCGAGTGCAtt  <  7:215664/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TGCCGCCCATTCCGATCTCGACATCATCACTATGGGCGCCAAAAGCAAGAACGTCAGTATTATACATTCGGCTCTTCTCCCCGAAGAAGGTCGCGGAATTCGAAAACACCTTCATCAAGTGTGCGGACAAGAATCTCCGCAGTACCCATATTTGTAGCGAGCGGAATGGAATACACGTCACATAACCGGATTAATGCCGAGACATCCGGTTCATGCGGCTGCGCGGTCAGCGGGTCGCGCAAAAAGATGACAAGATCGAGTGCATT  >  CP006881/2176704‑2176969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑