Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,177,069	T→G	T37T (ACA→ACC)	U712_10890 ←	Methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,177,069	0	T	G	98.6%	224.5 / NA	71	T37T (ACA→ACC)	U712_10890	Methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (28/42);  minor base C (0/1);  total (28/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCGCAAAAAGATGACAAGATCGAGTGCATTGGCCGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCTTGAATTTTCAACCCTGTTGTGCCGGTTGCGTATAGATCATGATGCCTCAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATCATTTATCCCCCTGTCTAATCAATGAT  >  CP006881/2176937‑2177203                                                                                                                                     |                                                                                                                                       gTCGCGCAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCg                                                                                                                                  <  7:114523/139‑1 (MQ=255) gTCGCGCAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCg                                                                                                                                  <  6:278311/139‑1 (MQ=255)   cgcgCAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGt                                                                                                                                <  3:373141/139‑1 (MQ=255)    gcgcAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTcccg                                                                                                                                 >  4:199101/1‑135 (MQ=255)    gcgcAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCgg                                                                                                                                 <  3:199101/137‑1 (MQ=255)    gcgcAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGtt                                                                                                                               <  8:129984/139‑1 (MQ=255)    gcgcAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGtt                                                                                                                               <  5:150343/139‑1 (MQ=255)    gcgcAAAAAAATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGtt                                                                                                                               >  7:53019/1‑139 (MQ=255)        aaaaaCATGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCTGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGt                                                                                                                           <  2:292866/139‑1 (MQ=255)         aaaaaaTGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGta                                                                                                                          <  7:293418/139‑1 (MQ=255)         aaaaaaTGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGta                                                                                                                          <  1:28521/139‑1 (MQ=255)         aaaaaaGGACAAGATCGAGTGCATTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGta                                                                                                                          <  6:70220/139‑1 (MQ=255)                               aTTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAata                                                                                                    <  7:415241/139‑1 (MQ=255)                                   gcagcgATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCtgtggttc                                                                                                                                    <  7:272748/100‑3 (MQ=255)                                   gcagcgATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCtgtggttc                                                                                                                                    >  8:272748/4‑101 (MQ=255)                                        gATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAg                                                                                           >  7:386210/1‑139 (MQ=255)                                         ctgaGTGCACCGATTTGCTGGTATCCCCCTAAAGGGCCGGATTGAAAACGTTCATTTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGGTGCGGATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          <  4:11936/136‑1 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGATTTTTCAACCCTGTGGTTCCGGTTGCGTAAAGATCATGATTGATTAAAATATACCGATAgg                                                                                          >  2:263234/1‑139 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          <  6:50804/139‑1 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          <  7:649/139‑1 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          >  8:75221/1‑139 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          <  3:367510/139‑1 (MQ=255)                                         aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                          <  6:402728/139‑1 (MQ=255)                                                cACCGATTTGCTGGTCTCCCCATAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGt                                                                                   <  8:407441/139‑1 (MQ=255)                                                   cGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAAAATCCCGATAGGCAGTCGTaaa                                                                                <  2:394331/139‑1 (MQ=255)                                                     aTTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                              <  4:206354/139‑1 (MQ=255)                                                     aTTTGCTGGGCTCCCCCTAAAGGGCAGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                              <  3:95218/139‑1 (MQ=255)                                                     aTTTGCGGGTCTCCCCCTAAAGGGCCTGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                              <  8:231797/139‑1 (MQ=255)                                                        tGCTGGTCTCCCCCTAAAGGGCCGGATTGAAACCGTTCAATTTGAAGACCTGTCGCCTCATGAAGTTTCAACCCTGTGGTTCCGGTTGCGTTAACATAAAGATTGTTTAAAATATGCCGTTTGGCAGTCGGAAACTGaa                                                                           >  2:156163/1‑139 (MQ=255)                                                        tGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGGTCCGGTTGCGGACTGATCATGATTCTTTTAAATATCCCCATAGGCAGTCGTAAATTGaa                                                                           >  2:156161/1‑139 (MQ=255)                                                        tGCTGGTCTCCCACTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTAAACCCAGTGGTTCCGGTTGCGTATAGATCATGATTCATTAAAATATACCGATAGGCAGTCTTAAATTGa                                                                            >  8:331293/1‑138 (MQ=255)                                                               ctcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                              >  3:200498/1‑129 (MQ=255)                                                               ctcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                              <  4:200498/129‑1 (MQ=255)                                                               cagccccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATc                                                                    <  3:303696/136‑1 (MQ=255)                                                                tcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTTAAATATCCCGATAGGCAGTCGTAAATTGAACCagatgc                                                                   >  1:394829/1‑137 (MQ=255)                                                                  ccccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGta                                                                                                                          <  6:327149/82‑1 (MQ=255)                                                                  ccccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGta                                                                                                                          >  5:327149/1‑82 (MQ=255)                                                                  acacTAAAGGGCCTGATTTAAAACGTTAAATTTGAAGACGTGTCTCCTCTTTAATTTTCTACAATGTGGTTCCGGTTTATTATAGATTATGTTTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCt                                                                  <  4:224176/135‑1 (MQ=255)                                                                    ccTTAAGGGCCGGATTGAAAACGTTCAATTTGACGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGCTAGGCAGTCGTAAATTGAACCATATCCTGtt                                                               <  4:161787/139‑1 (MQ=255)                                                                    ccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGtt                                                               <  2:51527/139‑1 (MQ=255)                                                                    ccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTAAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTAGTAAATTGAACCATATCCTGtt                                                               <  7:331293/139‑1 (MQ=255)                                                                       aaaGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATACCGATAGGCAGTCGTAAATTGAACCATATCCTGttttt                                                            >  7:208681/1‑139 (MQ=255)                                                                            gCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTg                                                       >  3:146615/1‑139 (MQ=255)                                                                            gCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCAGTGGTTCCGGTTGCGTATAGATCATGATTATTTAAAATATACCGATAGGCAGTCGTAAATTGAACCATAACCTGTATTTTATTg                                                       >  6:309625/1‑139 (MQ=255)                                                                             ccGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGAt                                                                                                                     >  5:151470/1‑76 (MQ=255)                                                                             ccGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGAt                                                                                                                     <  6:151470/76‑1 (MQ=255)                                                                             ccGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATAATGATTCTTTAAAATATACCGATAGGCAGTCGTAAATTTAACCATATCCTGttttttatctg                                                      >  4:303696/1‑134 (MQ=255)                                                                              cGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTTAAATATACCGATAGGCAGTCGTAAATTGAACCATATACTGTTTTTTCTTGt                                                      >  6:375679/1‑138 (MQ=255)                                                                                gATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCAt                                                   >  4:190486/1‑139 (MQ=255)                                                                                gATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCAt                                                   >  3:372200/1‑139 (MQ=255)                                                                                      aaaCGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTGGTTGTCATGCGCGa                                             >  6:320145/1‑139 (MQ=255)                                                                                       aaCGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGAt                                            <  8:138227/139‑1 (MQ=255)                                                                                         cGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                          <  3:203102/139‑1 (MQ=255)                                                                                           ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATATATCATTATTCTTTTAAATATCCCGATAGGCAGGGGTAAATTTAACAATATACAGTTTTTTGTTTTCATGCGCCATaaa                                         >  4:157268/1‑138 (MQ=255)                                                                                           ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                          >  1:244895/1‑137 (MQ=255)                                                                                           ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                          >  5:408083/1‑137 (MQ=255)                                                                                             ccaTTTGAAGACCTGTCGCCTCATGACTTATCAACCCTGTCGTTCCGGTTGCGTAGAGATCCTGAGTCTATAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGCTTTTTCTTGTCATGCGCGATCaaa                                        <  8:229045/135‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGTATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  8:103499/139‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  4:146615/139‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  3:261227/139‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  1:5165/139‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  1:263234/139‑1 (MQ=255)                                                                                                     aGACCTGTCGCCTCATGAAATTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  4:149999/139‑1 (MQ=255)                                                                                                     aGACCGGTCGCCTCATGAAGTTTCAACCCTGTGGTTCCGGGTGCGTATAGATCATGATTCGTTAAAATATCCCGATAGGCAGTCGTAAATTGTACCATATCCTGTGTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  2:159787/139‑1 (MQ=255)                                                                                                          tGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtcattgtt                         >  8:9836/1‑135 (MQ=255)                                                                                                          tGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtcattgtt                         >  1:118785/1‑135 (MQ=255)                                                                                                                ctcaTGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATccc                   <  1:131813/139‑1 (MQ=255)                                                                                                                   atGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATcccccc                >  6:372922/2‑138 (MQ=255)                                                                                                                          ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              <  6:44164/118‑1 (MQ=255)                                                                                                                          ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                              >  5:44164/1‑118 (MQ=255)                                                                                                                          ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTaa          >  4:295663/1‑138 (MQ=255)                                                                                                                                 cTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGat  <  5:43655/139‑1 (MQ=255)                                                                                                                                 cTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCACGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGat  <  4:378586/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTCGCGCAAAAAGATGACAAGATCGAGTGCATTGGCCGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCTTGAATTTTCAACCCTGTTGTGCCGGTTGCGTATAGATCATGATGCCTCAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATCATTTATCCCCCTGTCTAATCAATGAT  >  CP006881/2176937‑2177203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑