Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,177,097	C→T	R28K (AGG→AAG)	U712_10890 ←	Methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,177,097	0	C	T	97.3%	222.9 / NA	75	R28K (AGG→AAG)	U712_10890	Methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (29/43);  minor base A (2/0);  total (31/44)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGGCCGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCTTGAATTTTCAACCCTGTTGTGCCGGTTGCGTATAGATCATGATGCCTCAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATCATTTATCCCCCTGTCTAATCAATGATATTTTCTAATCCGTACACAAGCTGA  >  CP006881/2176967‑2177228                                                                                                                                   |                                                                                                                                    aTTGGCAGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAata                                                                                                                             <  7:415241/139‑1 (MQ=255)          gATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAg                                                                                                                    >  7:386210/1‑139 (MQ=255)           ctgaGTGCACCGATTTGCTGGTATCCCCCTAAAGGGCCGGATTGAAAACGTTCATTTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGGTGCGGATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   <  4:11936/136‑1 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGATTTTTCAACCCTGTGGTTCCGGTTGCGTAAAGATCATGATTGATTAAAATATACCGATAgg                                                                                                                   >  2:263234/1‑139 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   <  6:402728/139‑1 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   <  6:50804/139‑1 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   <  7:649/139‑1 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   <  3:367510/139‑1 (MQ=255)           aTCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAgg                                                                                                                   >  8:75221/1‑139 (MQ=255)                  cACCGATTTGCTGGTCTCCCCATAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGt                                                                                                            <  8:407441/139‑1 (MQ=255)                     cGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAAAATCCCGATAGGCAGTCGTaaa                                                                                                         <  2:394331/139‑1 (MQ=255)                       aTTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                                                       <  4:206354/139‑1 (MQ=255)                       aTTTGCTGGGCTCCCCCTAAAGGGCAGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                                                       <  3:95218/139‑1 (MQ=255)                       aTTTGCGGGTCTCCCCCTAAAGGGCCTGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                                                       <  8:231797/139‑1 (MQ=255)                          tGCTGGTCTCCCCCTAAAGGGCCGGATTGAAACCGTTCAATTTGAAGACCTGTCGCCTCATGAAGTTTCAACCCTGTGGTTCCGGTTGCGTTAACATAAAGATTGTTTAAAATATGCCGTTTGGCAGTCGGAAACTGaa                                                                                                    >  2:156163/1‑139 (MQ=255)                          tGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGGTCCGGTTGCGGACTGATCATGATTCTTTTAAATATCCCCATAGGCAGTCGTAAATTGaa                                                                                                    >  2:156161/1‑139 (MQ=255)                          tGCTGGTCTCCCACTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTAAACCCAGTGGTTCCGGTTGCGTATAGATCATGATTCATTAAAATATACCGATAGGCAGTCTTAAATTGa                                                                                                     >  8:331293/1‑138 (MQ=255)                                 ctcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                                                       >  3:200498/1‑129 (MQ=255)                                 ctcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAAtt                                                                                                       <  4:200498/129‑1 (MQ=255)                                 cagccccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATc                                                                                             <  3:303696/136‑1 (MQ=255)                                  tcCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTTAAATATCCCGATAGGCAGTCGTAAATTGAACCagatgc                                                                                            >  1:394829/1‑137 (MQ=255)                                    acacTAAAGGGCCTGATTTAAAACGTTAAATTTGAAGACGTGTCTCCTCTTTAATTTTCTACAATGTGGTTCCGGTTTATTATAGATTATGTTTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCt                                                                                           <  4:224176/135‑1 (MQ=255)                                      ccTTAAGGGCCGGATTGAAAACGTTCAATTTGACGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGCTAGGCAGTCGTAAATTGAACCATATCCTGtt                                                                                        <  4:161787/139‑1 (MQ=255)                                      ccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGtt                                                                                        <  2:51527/139‑1 (MQ=255)                                      ccTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTAAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTAGTAAATTGAACCATATCCTGtt                                                                                        <  7:331293/139‑1 (MQ=255)                                         aaaGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATACCGATAGGCAGTCGTAAATTGAACCATATCCTGttttt                                                                                     >  7:208681/1‑139 (MQ=255)                                              gCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTg                                                                                >  3:146615/1‑139 (MQ=255)                                              gCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCAGTGGTTCCGGTTGCGTATAGATCATGATTATTTAAAATATACCGATAGGCAGTCGTAAATTGAACCATAACCTGTATTTTATTg                                                                                >  6:309625/1‑139 (MQ=255)                                               ccGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATAATGATTCTTTAAAATATACCGATAGGCAGTCGTAAATTTAACCATATCCTGttttttatctg                                                                               >  4:303696/1‑134 (MQ=255)                                                cGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTTAAATATACCGATAGGCAGTCGTAAATTGAACCATATACTGTTTTTTCTTGt                                                                               >  6:375679/1‑138 (MQ=255)                                                  gATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCAt                                                                            >  4:190486/1‑139 (MQ=255)                                                  gATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCAt                                                                            >  3:372200/1‑139 (MQ=255)                                                        aaaCGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTGGTTGTCATGCGCGa                                                                      >  6:320145/1‑139 (MQ=255)                                                         aaCGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGAt                                                                     <  8:138227/139‑1 (MQ=255)                                                           cGTTCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                                                   <  3:203102/139‑1 (MQ=255)                                                             ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATATATCATTATTCTTTTAAATATCCCGATAGGCAGGGGTAAATTTAACAATATACAGTTTTTTGTTTTCATGCGCCATaaa                                                                  >  4:157268/1‑138 (MQ=255)                                                             ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                                                   >  5:408083/1‑137 (MQ=255)                                                             ttCAATTTGAAGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCa                                                                   >  1:244895/1‑137 (MQ=255)                                                               ccaTTTGAAGACCTGTCGCCTCATGACTTATCAACCCTGTCGTTCCGGTTGCGTAGAGATCCTGAGTCTATAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGCTTTTTCTTGTCATGCGCGATCaaa                                                                 <  8:229045/135‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGTATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  8:103499/139‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  1:5165/139‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  1:263234/139‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  4:146615/139‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  3:261227/139‑1 (MQ=255)                                                                       aGACCTGTCGCCTCATGAAATTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  4:149999/139‑1 (MQ=255)                                                                       aGACCGGTCGCCTCATGAAGTTTCAACCCTGTGGTTCCGGGTGCGTATAGATCATGATTCGTTAAAATATCCCGATAGGCAGTCGTAAATTGTACCATATCCTGTGTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  2:159787/139‑1 (MQ=255)                                                                            tGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtcattgtt                                                  >  1:118785/1‑135 (MQ=255)                                                                            tGTCGCCTCATGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtcattgtt                                                  >  8:9836/1‑135 (MQ=255)                                                                                  ctcaTGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATccc                                            <  1:131813/139‑1 (MQ=255)                                                                                     atGAATTTTCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATcccccc                                         >  6:372922/2‑138 (MQ=255)                                                                                            ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       >  5:44164/1‑118 (MQ=255)                                                                                            ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTtca                                                       <  6:44164/118‑1 (MQ=255)                                                                                            ttCAACCCTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTaa                                   >  4:295663/1‑138 (MQ=255)                                                                                                   cTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGat                           <  5:43655/139‑1 (MQ=255)                                                                                                   cTGTGGTTCCGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCACGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGat                           <  4:378586/139‑1 (MQ=255)                                                                                                      tggttccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATAtt                        >  5:102693/6‑139 (MQ=255)                                                                                                          tccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAATCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  5:287675/138‑1 (MQ=255)                                                                                                           ccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  3:105516/138‑1 (MQ=255)                                                                                                           ccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  3:12659/138‑1 (MQ=255)                                                                                                           ccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  3:238591/138‑1 (MQ=255)                                                                                                           ccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    >  4:105516/1‑138 (MQ=255)                                                                                                           ccGGTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  7:9836/138‑1 (MQ=255)                                                                                                              gTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTaaac                >  7:433449/1‑137 (MQ=255)                                                                                                              gTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTaaac                <  2:244895/139‑3 (MQ=255)                                                                                                              gTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTaaac                <  1:95044/139‑3 (MQ=255)                                                                                                              gTTGCGTATAGATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTaaac                <  6:408083/139‑3 (MQ=255)                                                                                                                  cGTATAGATCATGATTCTTTAAAATATACCGATAGGCAGTCGTAAATTGAACCAAATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTAACCCCCCCTATAATCAATTATAATTTCTAAAccat             >  5:176808/1‑136 (MQ=255)                                                                                                                        gATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTAAACCATACACAAg      <  3:190486/139‑1 (MQ=255)                                                                                                                        gATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTAAACCATACACAAg      <  8:208681/139‑1 (MQ=255)                                                                                                                        gATCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTAAACCATACACAAg      <  3:157268/139‑1 (MQ=255)                                                                                                                         aTCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    >  8:48393/1‑124 (MQ=255)                                                                                                                         aTCATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  7:48393/124‑1 (MQ=255)                                                                                                                           cATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    <  3:45731/122‑1 (MQ=255)                                                                                                                           cATGATTCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCt                    >  4:45731/1‑122 (MQ=255)                                                                                                                            atgattCTTTAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATTGTTTATCCCCCCGTCTAATCAATGATATTTTCTAAACCATACACAAGCTGa  >  5:95552/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                    ATTGGCCGCGATCAGTGCACCGATTTGCTGGTCTCCCCCTAAAGGGCCGGATTGAAAACGTTCAATTTGAAGACCTGTCGCCTCTTGAATTTTCAACCCTGTTGTGCCGGTTGCGTATAGATCATGATGCCTCAAAATATCCCGATAGGCAGTCGTAAATTGAACCATATCCTGTTTTTTCTTGTCATGCGCGATCAAAGCAATTTTCATCATTTATCCCCCTGTCTAATCAATGATATTTTCTAATCCGTACACAAGCTGA  >  CP006881/2176967‑2177228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑