Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,177,642	C→T	E118E (GAG→GAA)	U712_10895 ←	Dihydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,177,642	0	C	T	100.0%	162.8 / NA	52	E118E (GAG→GAA)	U712_10895	Dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

TGCGTCAAGCTTCCGATCATGGTGTAGCTCAATAATCTCAACATCCTCAAAGTAGTTGGCAGCCATTTTCGAAAATTTCATCATCAGCACCGCACCAAGTGCAAAGTTTGGAGCGATGATGGCTCCGATTCCCTTCTCTTCTGTTAATGACGTAAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCAACAACCGGACGAACGCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCAGGCGTTGTTAAATC  >  CP006881/2177507‑2177770                                                                                                                                        |                                                                                                                                 tGCGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttct                                                                                                                               <  3:166552/139‑1 (MQ=255) tGCGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttct                                                                                                                               <  5:321494/139‑1 (MQ=255) tGCGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttct                                                                                                                               <  1:42407/139‑1 (MQ=255)   cGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttcttc                                                                                                                             >  2:8004/1‑139 (MQ=255)   cGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttcttc                                                                                                                             <  1:8004/139‑1 (MQ=255)   cGTCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttctt                                                                                                                              <  3:267530/138‑1 (MQ=255)     tCAAGCTTCTGGTCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTAATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATTGCTCCGAGACCTTTTTCTTCTg                                                                                                                           >  4:267530/1‑139 (MQ=255)             ctggtCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTGTGTTAAAGat                                                                                                                   >  2:28701/5‑138 (MQ=255)              tggtCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGatg                                                                                                                  >  2:372904/4‑137 (MQ=255)                gtCATGATGAAGCTCAATAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGat                                                                                                                   <  5:157955/135‑2 (MQ=255)                   atgatgAAGCTCACTAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGc                                                                                                             <  6:13823/139‑1 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTa                                                                                                                        <  3:54796/116‑1 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTa                                                                                                                        >  4:60764/1‑116 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTa                                                                                                                        <  3:60764/116‑1 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTa                                                                                                                        >  4:54796/1‑116 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAAt                                                                                                   >  1:66193/1‑137 (MQ=255)                               aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAAt                                                                                                   <  2:40509/137‑1 (MQ=255)                                 taatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttct                                                                                                                               <  4:344223/107‑1 (MQ=255)                                 taatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCtttttct                                                                                                                               >  3:344223/1‑107 (MQ=255)                                 taatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGc                                                                                               <  3:286092/139‑1 (MQ=255)                                      tcAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCt                                                                                           >  3:351243/1‑138 (MQ=255)                                        aaCATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCt                                                                                           <  1:372904/136‑1 (MQ=255)                                         aCATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTgag                                                                                        >  1:340462/1‑138 (MQ=255)                                             ccTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAAcc                                                                                   >  5:196194/1‑139 (MQ=255)                                             ccTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATAGCACCGAGCTCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAAcc                                                                                   >  1:252267/1‑139 (MQ=255)                                              cTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCCCCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAATATGTGAGCTCTTTTATATCAGCTTCTGAGAAAcag                                                                                  >  7:277939/1‑137 (MQ=255)                                               tCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGATACCgg                                                                                 >  8:429855/1‑139 (MQ=255)                                                 aaaaTAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgtt                                                                               <  6:171362/139‑1 (MQ=255)                                                 aaaaTAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgtt                                                                               <  2:80995/139‑1 (MQ=255)                                                    atagtTGGCAGCAATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgttgtt                                                                            <  6:347756/138‑1 (MQ=255)                                                       gtTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                          <  7:72192/138‑1 (MQ=255)                                                        ttGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                          <  7:144751/137‑1 (MQ=255)                                                        ttGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                          >  8:144751/1‑137 (MQ=255)                                                         tGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTATGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgttgttacta                                                                        >  8:143352/1‑134 (MQ=255)                                                             aGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTTAGAAACCGGTTGTTCCgacaact                                                                   >  5:312440/1‑138 (MQ=255)                                                                 aTTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                               <  2:290875/139‑1 (MQ=255)                                                                     ttgAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACgga                                                            <  5:9732/136‑3 (MQ=255)                                                                         aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                       <  2:340462/139‑1 (MQ=255)                                                                         aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                       <  2:89560/139‑1 (MQ=255)                                                                         aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                       <  8:349450/139‑1 (MQ=255)                                                                                 tcatcaGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTaa                                               <  6:196194/139‑1 (MQ=255)                                                                                             caccGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgt                                   >  2:162856/1‑139 (MQ=255)                                                                                             caccGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATAAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtg                                    >  3:335915/1‑138 (MQ=255)                                                                                                cgagcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                <  1:162856/134‑1 (MQ=255)                                                                                                 gagcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                >  5:195886/5‑138 (MQ=255)                                                                                                  cgcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGATACCGGTTGTTCGGCCATCTGGACGGCCTCCGTGCTCTAATGCAATTTTtgtatgta                              >  7:259039/4‑139 (MQ=255)                                                                                                  agcggaaaaTTTGGCGCGATGATGGCTCCGATCCCTGTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCGTCTGAGAAACCGGTTGTTCCGACACCTGGACGGAGTCCGTGCTCTAATGCAATTTTtgtatg                                >  2:71260/6‑137 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGCTCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                              >  8:375171/4‑139 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                               <  1:235218/103‑1 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                               >  2:235218/4‑106 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                              >  1:192981/4‑139 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                              >  1:216012/4‑139 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                              >  3:126348/4‑139 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                >  3:125217/4‑137 (MQ=255)                                                                                                  agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTGGTGCCGACAACTGGACGGACTCCGTGCTCTACTGCAATTTTtgtatg                                >  3:245601/4‑137 (MQ=255)                                                                                                          atttGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCg                      <  3:290010/138‑1 (MQ=255)                                                                                                              ggcgCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGAttt                  >  1:199171/4‑139 (MQ=255)                                                                                                               gcgCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGAttt                  <  4:351243/136‑1 (MQ=255)                                                                                                                         ggCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCgttgt        >  5:330634/1‑138 (MQ=255)                                                                                                                               gATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATc  >  8:315128/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                 TGCGTCAAGCTTCCGATCATGGTGTAGCTCAATAATCTCAACATCCTCAAAGTAGTTGGCAGCCATTTTCGAAAATTTCATCATCAGCACCGCACCAAGTGCAAAGTTTGGAGCGATGATGGCTCCGATTCCCTTCTCTTCTGTTAATGACGTAAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCAACAACCGGACGAACGCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCAGGCGTTGTTAAATC  >  CP006881/2177507‑2177770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑