Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,177,660 A→G L112L (CTT→CTC)  U712_10895 ← Dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,177,6600AG100.0% 165.2 / NA 51L112L (CTT→CTC) U712_10895Dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/21);  total (30/21)

ATGGTGTAGCTCAATAATCTCAACATCCTCAAAGTAGTTGGCAGCCATTTTCGAAAATTTCATCATCAGCACCGCACCAAGTGCAAAGTTTGGAGCGATGATGGCTCCGATTCCCTTCTCTTCTGTTAATGACGTAAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCAACAACCGGACGAACGCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCAGGCGTTGTTAAATCAATTAAGACATCCGGCT  >  CP006881/2177525‑2177787
                                                                                                                                       |                                                                                                                               
atgatgAAGCTCACTAATCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGc                                                                                                                              <  6:13823/139‑1 (MQ=255)
            aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAAt                                                                                                                    >  1:66193/1‑137 (MQ=255)
            aataatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAAt                                                                                                                    <  2:40509/137‑1 (MQ=255)
              taatCTCAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGc                                                                                                                <  3:286092/139‑1 (MQ=255)
                   tcAACATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCt                                                                                                            >  3:351243/1‑138 (MQ=255)
                     aaCATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCt                                                                                                            <  1:372904/136‑1 (MQ=255)
                      aCATCCTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTgag                                                                                                         >  1:340462/1‑138 (MQ=255)
                          ccTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAAcc                                                                                                    >  5:196194/1‑139 (MQ=255)
                          ccTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATAGCACCGAGCTCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAAcc                                                                                                    >  1:252267/1‑139 (MQ=255)
                           cTCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCCCCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAATATGTGAGCTCTTTTATATCAGCTTCTGAGAAAcag                                                                                                   >  7:277939/1‑137 (MQ=255)
                            tCAAAATAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGATACCgg                                                                                                  >  8:429855/1‑139 (MQ=255)
                              aaaaTAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgtt                                                                                                <  6:171362/139‑1 (MQ=255)
                              aaaaTAGTTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgtt                                                                                                <  2:80995/139‑1 (MQ=255)
                                 atagtTGGCAGCAATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgttgtt                                                                                             <  6:347756/138‑1 (MQ=255)
                                    gtTGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                                           <  7:72192/138‑1 (MQ=255)
                                     ttGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                                           <  7:144751/137‑1 (MQ=255)
                                     ttGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTcc                                                                                           >  8:144751/1‑137 (MQ=255)
                                      tGGCAGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTATGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGgttgttacta                                                                                         >  8:143352/1‑134 (MQ=255)
                                          aGCCATTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTTAGAAACCGGTTGTTCCgacaact                                                                                    >  5:312440/1‑138 (MQ=255)
                                              aTTTTTGAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                                                <  2:290875/139‑1 (MQ=255)
                                                  ttgAAAATTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACgga                                                                             <  5:9732/136‑3 (MQ=255)
                                                      aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                                        <  2:89560/139‑1 (MQ=255)
                                                      aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                                        <  2:340462/139‑1 (MQ=255)
                                                      aaaTTTCATCATCAGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCg                                                                        <  8:349450/139‑1 (MQ=255)
                                                              tcatcaGTATCGCACCGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTaa                                                                <  6:196194/139‑1 (MQ=255)
                                                                          caccGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgt                                                    >  2:162856/1‑139 (MQ=255)
                                                                          caccGAGCGCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATAAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtg                                                     >  3:335915/1‑138 (MQ=255)
                                                                             cgagcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                                 <  1:162856/134‑1 (MQ=255)
                                                                              gagcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                                 >  5:195886/5‑138 (MQ=255)
                                                                               cgcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGATACCGGTTGTTCGGCCATCTGGACGGCCTCCGTGCTCTAATGCAATTTTtgtatgta                                               >  7:259039/4‑139 (MQ=255)
                                                                               agcggaaaaTTTGGCGCGATGATGGCTCCGATCCCTGTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCGTCTGAGAAACCGGTTGTTCCGACACCTGGACGGAGTCCGTGCTCTAATGCAATTTTtgtatg                                                 >  2:71260/6‑137 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGCTCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                                               >  8:375171/4‑139 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                                                >  2:235218/4‑106 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGAc                                                                                <  1:235218/103‑1 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                                               >  1:216012/4‑139 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                                               >  3:126348/4‑139 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatgta                                               >  1:192981/4‑139 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTtgtatg                                                 >  3:125217/4‑137 (MQ=255)
                                                                               agcgCAAAATTTGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTGGTGCCGACAACTGGACGGACTCCGTGCTCTACTGCAATTTTtgtatg                                                 >  3:245601/4‑137 (MQ=255)
                                                                                       atttGGCGCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCg                                       <  3:290010/138‑1 (MQ=255)
                                                                                           ggcgCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGAttt                                   >  1:199171/4‑139 (MQ=255)
                                                                                            gcgCGATGATGGCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGAttt                                   <  4:351243/136‑1 (MQ=255)
                                                                                                      ggCTCCGATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCgttgt                         >  5:330634/1‑138 (MQ=255)
                                                                                                            gATCCCTTTTTCTTCTGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATc                   >  8:315128/1‑138 (MQ=255)
                                                                                                                      tcttctGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACa         <  8:277939/138‑1 (MQ=255)
                                                                                                                      tcttctGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACa         <  6:330634/138‑1 (MQ=255)
                                                                                                                        ttctGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACATcc      >  7:190762/1‑139 (MQ=255)
                                                                                                                          ctGTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACATCCgg    >  7:19357/1‑139 (MQ=255)
                                                                                                                            gTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACATCCggtt  >  1:188936/1‑137 (MQ=255)
                                                                                                                            gTTAAAGATGTGAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCGGGCGTTGTTAAATCAATCAAGACATCCggtt  >  2:27647/1‑137 (MQ=255)
                                                                                                                            gTTAAAGATGTGAGCTCTATTAAATCAGCTTATGAGAAACCGGTTGTTCCGACAACTGGACGGACTCCGTGCTCTAATGAAATTTTAGTATCTACTTTTCCGATTACGTGAGTTCTTAAATAAATCAAGACATcctttt  >  8:259935/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                               
ATGGTGTAGCTCAATAATCTCAACATCCTCAAAGTAGTTGGCAGCCATTTTCGAAAATTTCATCATCAGCACCGCACCAAGTGCAAAGTTTGGAGCGATGATGGCTCCGATTCCCTTCTCTTCTGTTAATGACGTAAGCTCTTTTAAATCAGCTTCTGAGAAACCGGTTGTTCCAACAACCGGACGAACGCCGTGCTCTAATGCAATTTTTGTATGTACTTTTCCGATTTCAGGCGTTGTTAAATCAATTAAGACATCCGGCT  >  CP006881/2177525‑2177787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑