Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,178,238 T→A T35T (ACA→ACT)  U712_10900 ← Uncharacterized protein ypjD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,178,2380TA97.1% 201.5 / NA 70T35T (ACA→ACT) U712_10900Uncharacterized protein ypjD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (21/47);  minor base C (2/0);  total (23/47)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGTTGGCTAAACAAACCAATACAAACAGCACATCGCCCATTTCCTCTTCCATGCTTTTATCATCTTCAGACGCTTTTTTTGGTTTTTCTCCATAGCGATGATTCACTTCTCTGGCAAGCTCGCCAAGTTCTTCTGTCAGTCTCGCCATCATGGCAAGCGGGCTGAAATATCCTTCTTTAAATTGGCCGATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTAAACGAAACCTCCTATCATCCT  >  CP006881/2178103‑2178365
                                                                                                                                       |                                                                                                                               
agagTTGGCTAAACAAACCAATACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTTGTTTTTATGCATAAAGGGGAATAACTTATGAGGGCAGCTCGCCCAGTTGTTCAGt                                                                                                                               >  1:87230/1‑138 (MQ=255)
      ggCTAAACAAACCAATACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGcct                                                                                                                         <  3:404834/138‑1 (MQ=255)
       gCTAAACAAACCAATACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCg                                                                                                                       <  3:360199/139‑1 (MQ=255)
       gCTAAACAAACCAATACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCg                                                                                                                       <  4:128116/139‑1 (MQ=255)
                 ccAATACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAAAGGTGATTCAATTCACTGGCAAGCACGCCCACTTCTTCCGTCAGCCTCGCAATCATGGc                                                                                                             >  2:240334/1‑139 (MQ=255)
                    aTACAAATAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAg                                                                                                          <  1:240334/139‑1 (MQ=255)
                        aaaTAGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCggg                                                                                                      <  8:125777/139‑1 (MQ=255)
                          ataGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTTGTTTTTATGCATAACGGTGATTAACTTGTCTTGCAAGCTCGGCCAGTTAGTCCGTAAGCCTCGCCCTACTGGCCCGCGGGCt                                                                                                    >  8:252478/3‑139 (MQ=255)
                          ataGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTtcagtcagc                                                                                                                           <  8:2513/114‑2 (MQ=255)
                          ataGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTtcagtcagc                                                                                                                           >  7:2513/3‑115 (MQ=255)
                           tcccaccTCGCCTATTTCCTCTTCCATGCTTTTATCATCTACAGACGCGTATTCGGGTATTTCTCCCTAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCGTCAGTCAGCCTCGCCATCATGGCCAGCGGGCta                                                                                                   <  4:200293/136‑2 (MQ=255)
                            aGCACATCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCGTTTTTTTGTTTTTATCCATAACGGTGATTGAATTATCTGGCAAGCTCGCCCAGGTGTTCATTCAGCCTCGCCCACATGGCCAGCGGGCta                                                                                                   >  3:43470/1‑137 (MQ=255)
                                  tCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATc                                                                                            <  1:68382/139‑1 (MQ=255)
                                  tCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATc                                                                                            <  5:67528/139‑1 (MQ=255)
                                  tCGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCGCGCCCAGTTCTTCAGTAAGCCGCGCCAACAAGGCCAGCCGGCTaaaaaaac                                                                                            >  2:68382/1‑135 (MQ=255)
                                   cGCCTATTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGGTTTTCTCCATAACGGTGATTAACTTCTCTGGCCAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCCTCAAGGCCAGCGGGCTAAAATATcc                                                                                           >  7:95061/1‑139 (MQ=255)
                                     cctaTTTCCTCTTCCATGCTTTTATCATCTTCAGTCGCGTTTTTTTGTTTTTATCCAAAACGGGGATTCACGTCTAATGCCAGCTCGCCCACTTATTCAGTCAGCCGAGCCCGCATGGCCCGCCGGCGAAAATATcgg                                                                                          >  1:317290/4‑136 (MQ=255)
                                             ctctTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTaaa                                                                                  <  4:199259/138‑1 (MQ=255)
                                             ctctTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTaaa                                                                                  <  6:423668/138‑1 (MQ=255)
                                             ctctTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACATCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTaaa                                                                                  <  2:215606/138‑1 (MQ=255)
                                               ctTCCATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTg                                                                               >  4:148861/1‑139 (MQ=255)
                                                ttCCATGCTTTTATCATCTTCAGTCGCTTTTTTTTGTTTTTCTCCAAAACGGTGATTAAATTATCTGGCAAGCTCGCCCAGTTCTTCAGTCCGCCTCGCCCTCAAGGCCAGCGGGCTAAAATATCCGTCTTTAAATTgt                                                                              >  8:351676/1‑138 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  4:72221/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  6:312815/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  7:316178/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  7:351676/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  5:23321/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  3:71833/138‑1 (MQ=255)
                                                  ccATGCTTTTATCATCTTCAGTCGCGTTTTTTGGTTTTTATACATAAACGTGATTCAATTCTCAGGGAAGCGCGCCCAGGTGTTAAGTCAGGCTCGCCATCAAGGCCCGCCGGCTAAAATATACGTTTTTAAATTgggc                                                                            >  4:121568/1‑137 (MQ=255)
                                                   cATGCTTTTATCATCTTCAGTCGCTTTTTTTTGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTAAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGcc                                                                            >  5:198626/1‑138 (MQ=255)
                                                     tGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTGACTTCTCTGGCAACCTCGCCCCGGTGTTCAGTCCGCCTCGCCAGAATGGCCCGAGGGCTAAAAAATCCTTTTTTTAATTGGCCTAt                                                                         >  6:241380/1‑139 (MQ=255)
                                                     tGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTaaaa                                                                                                <  6:350313/116‑1 (MQ=255)
                                                     tGCTTTTATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTaaaa                                                                                                >  5:350313/1‑116 (MQ=255)
                                                          ttATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     <  8:360887/138‑1 (MQ=255)
                                                          ttATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     <  1:33374/138‑1 (MQ=255)
                                                          ttATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     <  2:335530/138‑1 (MQ=255)
                                                          ttATCATCTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     <  6:185149/138‑1 (MQ=255)
                                                             tcatcTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGt                                                                 <  8:202734/139‑1 (MQ=255)
                                                             tcatcTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGt                                                                 <  5:241380/139‑1 (MQ=255)
                                                             tcatcTTCAGTCGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGt                                                                 <  3:148861/139‑1 (MQ=255)
                                                                    catgcGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACtt                                                           <  4:43470/134‑1 (MQ=255)
                                                                    cAGTCGCTTTTTTTTGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGTCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACtt                                                           <  6:318369/138‑1 (MQ=255)
                                                                    cAGTCGCTTTTTTTGGTGTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACtt                                                           <  7:253321/138‑1 (MQ=255)
                                                                       tcGCTTTTTTTGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTAGCCCAGTTCTTCAGTCAGCCTCGCCATAATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGc                                                       >  8:372289/2‑139 (MQ=255)
                                                                             tttttGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGt                                                   <  8:95061/137‑1 (MQ=255)
                                                                              ttttGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTAt                                                 <  7:108823/138‑1 (MQ=255)
                                                                                 tGGTTTTTCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTc                                              <  4:196156/138‑1 (MQ=255)
                                                                                      tttCTCCTTAAGGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGGCAGCCTCGCCATCATGGCGAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCa                                         <  6:5609/138‑1 (MQ=255)
                                                                                       ttCTCCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCAt                                        <  3:228140/138‑1 (MQ=255)
                                                                                       tgctcCATAACGGTGATTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCAt                                        <  4:291423/136‑1 (MQ=255)
                                                                                                  ggtgatTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTAtcact                            <  1:188970/137‑1 (MQ=255)
                                                                                                    tgatTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             >  7:69749/1‑88 (MQ=255)
                                                                                                    tgatTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGc                                                                             <  8:69749/88‑1 (MQ=255)
                                                                                                    tgatTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTAtcactca                          >  4:157580/1‑139 (MQ=255)
                                                                                                      atTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     <  8:354118/94‑1 (MQ=255)
                                                                                                      atTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTaa                                                                     >  7:354118/1‑94 (MQ=255)
                                                                                                      atTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATcactcact                        >  3:133436/1‑139 (MQ=255)
                                                                                                      atTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATcactcact                        >  1:247761/1‑139 (MQ=255)
                                                                                                      atTCACTTCTCTGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATcactcact                        >  2:116785/1‑139 (MQ=255)
                                                                                                                 tGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTAtca                              >  5:334594/1‑122 (MQ=255)
                                                                                                                 tGGCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTAtca                              <  6:334594/122‑1 (MQ=255)
                                                                                                                   gCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAAcctc            <  2:317290/138‑1 (MQ=255)
                                                                                                                   gCAAGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAAcc              <  6:326262/136‑1 (MQ=255)
                                                                                                                      aGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTat        >  7:352816/1‑139 (MQ=255)
                                                                                                                      aGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTat        <  2:87230/139‑1 (MQ=255)
                                                                                                                      aGCTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTat        <  6:198626/139‑1 (MQ=255)
                                                                                                                        cTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAACTTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTatca      <  6:248323/139‑1 (MQ=255)
                                                                                                                        cTCGCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTatca      <  4:357023/139‑1 (MQ=255)
                                                                                                                           gCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTatcatc    <  3:123891/138‑1 (MQ=255)
                                                                                                                           gCCCAGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTatcat     <  2:247761/137‑1 (MQ=255)
                                                                                                                            cccaGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTATCATCct  >  1:276404/4‑139 (MQ=255)
                                                                                                                            cccaGTTCTTCAGTCAGCCTCGCCATCATGGCCAGCGGGCTAAAATATCCTTCTTTAAATTGGCCTATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTGAACGAAACCTCCTATCATCct  <  7:252478/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                               
AGAGTTGGCTAAACAAACCAATACAAACAGCACATCGCCCATTTCCTCTTCCATGCTTTTATCATCTTCAGACGCTTTTTTTGGTTTTTCTCCATAGCGATGATTCACTTCTCTGGCAAGCTCGCCAAGTTCTTCTGTCAGTCTCGCCATCATGGCAAGCGGGCTGAAATATCCTTCTTTAAATTGGCCGATGTAACGGTCTACTTCAGCCTGTATGTCTTTCATTGTTTTATCACTCACTAAACGAAACCTCCTATCATCCT  >  CP006881/2178103‑2178365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑